Q09YI0 (TES_SAIBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testin

Gene names

Name:

TES

Organism

Saimiri boliviensis boliviensis (Bolivian squirrel monkey)

Taxonomic identifier

39432 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Platyrrhini › Cebidae › Saimiriinae › Saimiri › Saimiri boliviensis

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor By similarity.
Subunit structure	Interacts via LIM domain 1 with ZYX. Interacts (via LIM domain 3) with ENAH and VASP. Interacts with talin, actin, alpha-actinin, GRIP1 and PXN By similarity. Interacts (via LIM domain 2) with ACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimer interacts with ENAH to form a heterotrimer By similarity.
Subcellular location	Cytoplasm By similarity. Cell junction › focal adhesion By similarity. Note: Detected along actin stress fibers By similarity.
Domain	The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX By similarity.
Sequence similarities	Belongs to the prickle / espinas / testin family. Contains 3 LIM zinc-binding domains. Contains 1 PET domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
Gene Ontology (GO)
Biological_process	negative regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Testin		PRO_0000260337
Regions
	Domain	92 – 199	108	PET
	Domain	234 – 297	64	LIM zinc-binding 1
	Domain	299 – 359	61	LIM zinc-binding 2
	Domain	362 – 421	60	LIM zinc-binding 3

Sequences

	Sequence		Length	Mass (Da)
	Q09YI0 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 06665DCF03C039FB	FASTA	421	47,983
	10 20 30 40 50 60 MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE HDVLLSNEED 70 80 90 100 110 120 RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA KKNVSINTVT YEWAPPVHNQ 130 140 150 160 170 180 ALARQYMQML PKEKQPVAGS EGAQYRKKQL AKQLPAHDQD PSKCHELSPR EVKEMEQFVK 190 200 210 220 230 240 KYKSEALGVG DVKLPCEMDA QGPKKMYIPG GDRSTPPAAG AMEDKSAEHK STQYSCYCCK 250 260 270 280 290 300 LNMKEGDPAI YAERAGYDKL WHPACFVCSV CHELLVDMIY FWKNEKLYCG RHYCDSEKPR 310 320 330 340 350 360 CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK PCYVKNHAVV 370 380 390 400 410 420 CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG QKFMPVEGMV FCSVECKKMM S

References

[1]

"NISC comparative sequencing initiative." Antonellis A., Ayele K., Benjamin B., Blakesley R.W., Boakye A., Bouffard G.G., Brinkley C., Brooks S., Chu G., Coleman H., Engle J., Gestole M., Greene A., Guan X., Gupta J., Haghighi P., Han J., Hansen N. , Ho S.-L., Hu P., Hunter G., Hurle B., Idol J.R., Kwong P., Laric P., Larson S., Lee-Lin S.-Q., Legaspi R., Madden M., Maduro Q.L., Maduro V.B., Margulies E.H., Masiello C., Maskeri B., McDowell J., Mojidi H.A., Mullikin J.C., Oestreicher J.S., Park M., Portnoy M.E., Prasad A., Puri O., Reddix-Dugue N., Schandler K., Schueler M.G., Sison C., Stantripop S., Stephen E., Taye A., Thomas J.W., Thomas P.J., Tsipouri V., Ung L., Vogt J.L., Wetherby K.D., Young A., Green E.D. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000180 Genomic DNA. Translation: ABI75300.1.
RefSeq	XP_003921113.1. XM_003921064.1.
3D structure databases
ProteinModelPortal	Q09YI0.
SMR	Q09YI0. Positions 357-420.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	101040506.
Organism-specific databases
CTD	26136.
Phylogenomic databases
HOVERGEN	HBG001038.
Family and domain databases
Gene3D	2.10.110.10. 3 hits.
InterPro	IPR010442. PET_domain. IPR001781. Znf_LIM. [Graphical view]
Pfam	PF00412. LIM. 2 hits. PF06297. PET. 1 hit. [Graphical view]
SMART	SM00132. LIM. 3 hits. [Graphical view]
PROSITE	PS00478. LIM_DOMAIN_1. 2 hits. PS50023. LIM_DOMAIN_2. 3 hits. PS51303. PET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TES_SAIBB

Accession

Primary (citable) accession number: Q09YI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program