ID   USP1_SOYBN              Reviewed;         600 AA.
AC   Q09WE7;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   20-JAN-2009, entry version 14.
DE   RecName: Full=UDP-sugar pyrophosphorylase 1;
DE            EC=2.7.7.64;
GN   Name=USP1;
OS   Glycine max (Soybean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae;
OC   Glycine.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Lambert;
RX   AGRICOLA=IND43835961; DOI=10.1111/j.1399-3054.2006.00728.x;
RA   Litterer L.A., Plaisance K.L., Schnurr J.A., Storey K.K.,
RA   Jung H.-J.G., Gronwald J.W., Somers D.A.;
RT   "Biosynthesis of UDP-glucuronic acid in developing soybean embryos:
RT   possible role of UDP-sugar pyrophosphorylase.";
RL   Physiol. Plantarum 128:200-211(2006).
CC   -!- FUNCTION: May function as the terminal enzyme of the myo-inositol
CC       oxidation (MIO) pathway. May also play a role in the salvage
CC       pathway for synthesis of nucleotide sugars.
CC   -!- CATALYTIC ACTIVITY: UTP + a monosaccharide 1-phosphate =
CC       diphosphate + UDP-monosaccharide.
CC   -!- COFACTOR: Magnesium or manganese.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.14 mM for glucuronic acid-1-phosphate;
CC         KM=0.23 mM for glucose-1-phosphate;
CC         KM=0.17 mM for UTP;
CC         Vmax=5.03 nmol/min/mg enzyme for the forward reaction with
CC         glucuronic acid-1-phosphate as substrate;
CC         Vmax=4.99 nmol/min/mg enzyme for the forward reaction with
CC         glucose-1-phosphate as substrate;
CC         Note=High activity with galactose-1-phosphate, glucose-1-
CC         phosphate or glucuronic acid-1-phosphate, but low or no activity
CC         with N-acetylglucosamine-1-phosphate, mannose-1-phosphate or
CC         glucose-6-phosphate;
CC       pH dependence:
CC         Optimum pH is 7.5-9.0;
CC   -!- TISSUE SPECIFICITY: Expressed in root tips, young leaves, flowers
CC       and developing embryos.
CC   -!- SIMILARITY: Belongs to the USP family.
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DR   EMBL; DQ267699; ABB89732.1; -; mRNA.
DR   UniGene; Gma.28201; -.
DR   BRENDA; 2.7.7.44; 299.
DR   BRENDA; 2.7.7.64; 299.
DR   GO; GO:0051748; F:UTP-monosaccharide-1-phosphate uridylyltran...; IEA:EC.
DR   GO; GO:0008152; P:metabolic process; IEA:InterPro.
DR   InterPro; IPR002618; UDPGP_trans.
DR   PANTHER; PTHR11952; UDPGP_trans; 1.
PE   1: Evidence at protein level;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN         1    600       UDP-sugar pyrophosphorylase 1.
FT                                /FTId=PRO_0000289980.
SQ   SEQUENCE   600 AA;  66126 MW;  6261863E476584B4 CRC64;
     MASSLGDNFN LLSPQQQELV KMLLDNGQEH LFRDWPAPGV DDDHKNAFFD QLTRLDSSYP
     GGLEAYITNA KRLLADSKAG RNPFDGFTPS VPTGETLAFG DENYIKFEEA GVLEARKAAF
     VLVAGGLGER LGYSGIKLAL PAETTTRTCF VQNYIESILA LQEASSQGES QTQIPLVIMT
     SDDTHGRTLE LLESNSYFGM QPTQVTLLKQ EKVACLEDND ARLALEPQNK YKIQTKPHGH
     GDVHALLYSS GILKVWYEAG LKWVLFFQDT NGLLFKAIPS ALGVSAAKQY HVNSLAVPRK
     AKEAIGGITR LTHSDGRSMV INVEYNQLDP LLRASGYPDG DVNCETGYSP FPGNINQLIL
     ELGHYIEELS KTGGAIQEFV NPKYKDASKT SFKSSTRLEC MMQDYPKTLP PSARVGFTVM
     ETWLAYAPVK NNAEDAAKVP KGNPYHSATS GEMAIYRANS IILRKAGVQV ADPVVQVFNG
     QEVEVWPRIT WKPKWGLTFN RIKSKVSGNC SISLRSTLAI KGPNIFIENL SVDGALIIDA
     VDDAEVNVSG SVQNNGWVLE TVDYKDASEP EVLRIRGFKF NKIEQLETKY SEPGKFHLKA
//