Reviewed, UniProtKB/Swiss-Prot Q09WE7 (USP1_SOYBN)

Names and origin

Protein names

Recommended name:
    UDP-sugar pyrophosphorylase 1
    EC=2.7.7.64

Gene names

Name:

USP1

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Ref.1
Catalytic activity	UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.
Cofactor	Magnesium or manganese.
Tissue specificity	Expressed in root tips, young leaves, flowers and developing embryos. Ref.1
Sequence similarities	Belongs to the USP family.
biophysicochemical properties	Kinetic parameters: High activity with galactose-1-phosphate, glucose-1-phosphate or glucuronic acid-1-phosphate, but low or no activity with N-acetylglucosamine-1-phosphate, mannose-1-phosphate or glucose-6-phosphate. KM=0.14 mM for glucuronic acid-1-phosphate KM=0.23 mM for glucose-1-phosphate KM=0.17 mM for UTP Vmax=5.03 nmol/min/mg enzyme for the forward reaction with glucuronic acid-1-phosphate as substrate Vmax=4.99 nmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate pH dependence: Optimum pH is 7.5-9.0.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	UTP-monosaccharide-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	UDP-sugar pyrophosphorylase 1		PRO_0000289980

Sequences

	Sequence		Length	Mass (Da)
	Q09WE7-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 6261863E476584B4	FASTA	600	66,126
	10 20 30 40 50 60 MASSLGDNFN LLSPQQQELV KMLLDNGQEH LFRDWPAPGV DDDHKNAFFD QLTRLDSSYP 70 80 90 100 110 120 GGLEAYITNA KRLLADSKAG RNPFDGFTPS VPTGETLAFG DENYIKFEEA GVLEARKAAF 130 140 150 160 170 180 VLVAGGLGER LGYSGIKLAL PAETTTRTCF VQNYIESILA LQEASSQGES QTQIPLVIMT 190 200 210 220 230 240 SDDTHGRTLE LLESNSYFGM QPTQVTLLKQ EKVACLEDND ARLALEPQNK YKIQTKPHGH 250 260 270 280 290 300 GDVHALLYSS GILKVWYEAG LKWVLFFQDT NGLLFKAIPS ALGVSAAKQY HVNSLAVPRK 310 320 330 340 350 360 AKEAIGGITR LTHSDGRSMV INVEYNQLDP LLRASGYPDG DVNCETGYSP FPGNINQLIL 370 380 390 400 410 420 ELGHYIEELS KTGGAIQEFV NPKYKDASKT SFKSSTRLEC MMQDYPKTLP PSARVGFTVM 430 440 450 460 470 480 ETWLAYAPVK NNAEDAAKVP KGNPYHSATS GEMAIYRANS IILRKAGVQV ADPVVQVFNG 490 500 510 520 530 540 QEVEVWPRIT WKPKWGLTFN RIKSKVSGNC SISLRSTLAI KGPNIFIENL SVDGALIIDA 550 560 570 580 590 600 VDDAEVNVSG SVQNNGWVLE TVDYKDASEP EVLRIRGFKF NKIEQLETKY SEPGKFHLKA

References

[1]

"Biosynthesis of UDP-glucuronic acid in developing soybean embryos: possible role of UDP-sugar pyrophosphorylase." Litterer L.A., Plaisance K.L., Schnurr J.A., Storey K.K., Jung H.-J.G., Gronwald J.W., Somers D.A. Physiol. Plantarum 128:200-211(2006) [Agricola: IND43835961] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Strain: cv. Lambert.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ267699 mRNA. Translation: ABB89732.1.
UniGene	Gma.28201
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.44. 299. 2.7.7.64. 299.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. [Graphical view]
PANTHER	PTHR11952. UDPGP_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

USP1_SOYBN

Accession

Primary (citable) accession number: Q09WE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	October 17, 2006
Last modified:	January 20, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)