Q09M05 (CBPC4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic carboxypeptidase 4
EC=3.4.17.-

Alternative name(s):
ATP/GTP-binding protein-like 1

Gene names

Name:	Agbl1
Synonyms:	Ccp4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Ref.2
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with MYLK. Ref.2
Subcellular location	Cytoplasm › cytosol By similarity.
Tissue specificity	Expressed at low level. Expressed in eye, muscle, pituitary, testis and to a lower extent in brain. Ref.1 Ref.2
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C-terminal protein deglutamylation Inferred from direct assay Ref.2. Source: UniProtKB protein side chain deglutamylation Inferred from direct assay Ref.2. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metallocarboxypeptidase activity Inferred from direct assay Ref.2. Source: UniProtKB tubulin binding Inferred from direct assay Ref.2. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q09M05-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q09M05-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-206: Missing.
     850-857: FCDFHGHS → SHFLLFIL
     858-972: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q09M05-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-206: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q09M05-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAEQEGSGLQMLLHTLQNSSDKASTLSILQVLGDLLSVGTDRRIYYM
     952-972: VCEVYTARSLCSAADHKNRGK → GLQFGTGELE...GTGWRRRSVT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 972	972	Cytosolic carboxypeptidase 4		PRO_0000305000
Regions
	Compositional bias	256 – 283	28	Asp-rich
Sites
	Active site	807	1	Nucleophile By similarity
	Metal binding	757	1	Zinc Probable
	Metal binding	760	1	Zinc Probable
	Metal binding	854	1	Zinc By similarity
Natural variations
	Alternative sequence	1 – 206	206	Missing in isoform 2 and isoform 3.		VSP_028179
	Alternative sequence	1	1	M → MAEQEGSGLQMLLHTLQNSS DKASTLSILQVLGDLLSVGT DRRIYYM in isoform 4.		VSP_040424
	Alternative sequence	850 – 857	8	FCDFHGHS → SHFLLFIL in isoform 2.		VSP_028180
	Alternative sequence	858 – 972	115	Missing in isoform 2.		VSP_028181
	Alternative sequence	952 – 972	21	VCEVY…KNRGK → GLQFGTGELEEMGAMYCLGL LILELKSVNCSHKLLARASS LLNADVLEHYLQRCSSSSSN SSNRTSEVDDEPYCMEEIDY SADSSSDAEQNFTELDRQIQ ECALNKDEEEEEKEEGTGWR RRSVT in isoform 4.		VSP_040425
Experimental info
	Mutagenesis	757	1	H → S: Abolishes deglutamylase activity; when associated with Q-760. Ref.2
	Mutagenesis	760	1	E → Q: Abolishes deglutamylase activity; when associated with S-757. Ref.2
	Sequence conflict	130 – 133	4	SKHL → KSNC in CAZ69802. Ref.2
	Sequence conflict	197	1	S → I in CAZ69802. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: 35CA57FDEC4D12DB	FASTA	972	109,368
	10 20 30 40 50 60 MISKGGSEAL LQTLVDTARS SSPDWDILLP LFRLLAKVGL RDKKFGQKAL ELEALDVTLI 70 80 90 100 110 120 LARKNLSHSQ NLLHCLWVLR VFASSVTTGA MLGINGAMEL LFKVLSPYTR KHTRTIRAAT 130 140 150 160 170 180 EVLAALLKSS KHLRRAVNRG YVNSLLRLHQ DWHSRDVTNT YVTIRHGLLL CLRHIVALRS 190 200 210 220 230 240 GREAFLAAQG METLFSSAQT CLENKNMELV ISAVIQILRQ CYPASRLPLV TASSAYTFPA 250 260 270 280 290 300 PGSTSSELPL NLTEEDFDDD GDEEMDKDSD VEAVKEDDDL ETDLSKLSSK PGLDLPEEEL 310 320 330 340 350 360 AQYDAMCPEL SCSFEELEPK CGDDLNNKDT LHANHHHIPS VASLRQHCFN REHSSWRQER 370 380 390 400 410 420 EDTVHSSILH MVKTGKSGVP SSSKQRSATN VNQSLQQNGL EIDSSGHDTS DIQAPLEQAA 430 440 450 460 470 480 WDMEAISCPR ITASFPNSTK PEESIGAAEK LLHTHAKHIP FHDPHLYIAN AMRTRSAVGF 490 500 510 520 530 540 KTMAFPDLWG HCPPPAAQPM LDRKLGVQRI KILEDIRRLL HPSDVINKVV FSLDEPRPLQ 550 560 570 580 590 600 GSISNCLMFH SKFESGNLRK AIQVREFEYD LLVNADVNSS QHQQWFYFKV SGMRAAVPYH 610 620 630 640 650 660 FNIINCEKPN SQFNYGMQPT LYSVKEALLG RPAWIRTGSD ICYYKNHYRQ NAATMDGALG 670 680 690 700 710 720 KRYYTLTFAV TFPHNEDACY LAYHYPYTYS TLMTHLEILE RSIDHREIYF RHDVLCQTLG 730 740 750 760 770 780 GNPCPLVTIT AFPESNSTEH LEQFRCRPYQ VITARVHPGE SNASWVMKGT LEFLVSSDPV 790 800 810 820 830 840 AKLLRENFVF KIIPMLNPDG VINGNHRCSL RGEDLNRQWL SPQAHLQPTI YHAKGLLHYL 850 860 870 880 890 900 SSTGRGPVVF CDFHGHSQKK NVFLYGCSMK ETLWQAGCTV GESALLEDVS YRTLPKILDK 910 920 930 940 950 960 LAPAFTMNSC SFLVEKSRAS TARVVVWREM GVSRSYTMES SYCGCNQGPY QVCEVYTARS 970 LCSAADHKNR GK
	Isoform 2 [UniParc]. Checksum: DC8FA5375A1C1E3E	FASTA	651	73,608
	10 20 30 40 50 60 MELVISAVIQ ILRQCYPASR LPLVTASSAY TFPAPGSTSS ELPLNLTEED FDDDGDEEMD 70 80 90 100 110 120 KDSDVEAVKE DDDLETDLSK LSSKPGLDLP EEELAQYDAM CPELSCSFEE LEPKCGDDLN 130 140 150 160 170 180 NKDTLHANHH HIPSVASLRQ HCFNREHSSW RQEREDTVHS SILHMVKTGK SGVPSSSKQR 190 200 210 220 230 240 SATNVNQSLQ QNGLEIDSSG HDTSDIQAPL EQAAWDMEAI SCPRITASFP NSTKPEESIG 250 260 270 280 290 300 AAEKLLHTHA KHIPFHDPHL YIANAMRTRS AVGFKTMAFP DLWGHCPPPA AQPMLDRKLG 310 320 330 340 350 360 VQRIKILEDI RRLLHPSDVI NKVVFSLDEP RPLQGSISNC LMFHSKFESG NLRKAIQVRE 370 380 390 400 410 420 FEYDLLVNAD VNSSQHQQWF YFKVSGMRAA VPYHFNIINC EKPNSQFNYG MQPTLYSVKE 430 440 450 460 470 480 ALLGRPAWIR TGSDICYYKN HYRQNAATMD GALGKRYYTL TFAVTFPHNE DACYLAYHYP 490 500 510 520 530 540 YTYSTLMTHL EILERSIDHR EIYFRHDVLC QTLGGNPCPL VTITAFPESN STEHLEQFRC 550 560 570 580 590 600 RPYQVITARV HPGESNASWV MKGTLEFLVS SDPVAKLLRE NFVFKIIPML NPDGVINGNH 610 620 630 640 650 RCSLRGEDLN RQWLSPQAHL QPTIYHAKGL LHYLSSTGRG PVVSHFLLFI L
	Isoform 3 [UniParc]. Checksum: E8656F1875720724	FASTA	766	86,375
	10 20 30 40 50 60 MELVISAVIQ ILRQCYPASR LPLVTASSAY TFPAPGSTSS ELPLNLTEED FDDDGDEEMD 70 80 90 100 110 120 KDSDVEAVKE DDDLETDLSK LSSKPGLDLP EEELAQYDAM CPELSCSFEE LEPKCGDDLN 130 140 150 160 170 180 NKDTLHANHH HIPSVASLRQ HCFNREHSSW RQEREDTVHS SILHMVKTGK SGVPSSSKQR 190 200 210 220 230 240 SATNVNQSLQ QNGLEIDSSG HDTSDIQAPL EQAAWDMEAI SCPRITASFP NSTKPEESIG 250 260 270 280 290 300 AAEKLLHTHA KHIPFHDPHL YIANAMRTRS AVGFKTMAFP DLWGHCPPPA AQPMLDRKLG 310 320 330 340 350 360 VQRIKILEDI RRLLHPSDVI NKVVFSLDEP RPLQGSISNC LMFHSKFESG NLRKAIQVRE 370 380 390 400 410 420 FEYDLLVNAD VNSSQHQQWF YFKVSGMRAA VPYHFNIINC EKPNSQFNYG MQPTLYSVKE 430 440 450 460 470 480 ALLGRPAWIR TGSDICYYKN HYRQNAATMD GALGKRYYTL TFAVTFPHNE DACYLAYHYP 490 500 510 520 530 540 YTYSTLMTHL EILERSIDHR EIYFRHDVLC QTLGGNPCPL VTITAFPESN STEHLEQFRC 550 560 570 580 590 600 RPYQVITARV HPGESNASWV MKGTLEFLVS SDPVAKLLRE NFVFKIIPML NPDGVINGNH 610 620 630 640 650 660 RCSLRGEDLN RQWLSPQAHL QPTIYHAKGL LHYLSSTGRG PVVFCDFHGH SQKKNVFLYG 670 680 690 700 710 720 CSMKETLWQA GCTVGESALL EDVSYRTLPK ILDKLAPAFT MNSCSFLVEK SRASTARVVV 730 740 750 760 WREMGVSRSY TMESSYCGCN QGPYQVCEVY TARSLCSAAD HKNRGK
	Isoform 4 [UniParc]. Checksum: F9B818FB39F03B37	FASTA	1,122	126,149
	10 20 30 40 50 60 MAEQEGSGLQ MLLHTLQNSS DKASTLSILQ VLGDLLSVGT DRRIYYMISK GGSEALLQTL 70 80 90 100 110 120 VDTARSSSPD WDILLPLFRL LAKVGLRDKK FGQKALELEA LDVTLILARK NLSHSQNLLH 130 140 150 160 170 180 CLWVLRVFAS SVTTGAMLGI NGAMELLFKV LSPYTRKHTR TIRAATEVLA ALLKSSKHLR 190 200 210 220 230 240 RAVNRGYVNS LLRLHQDWHS RDVTNTYVTI RHGLLLCLRH IVALRSGREA FLAAQGMETL 250 260 270 280 290 300 FSSAQTCLEN KNMELVISAV IQILRQCYPA SRLPLVTASS AYTFPAPGST SSELPLNLTE 310 320 330 340 350 360 EDFDDDGDEE MDKDSDVEAV KEDDDLETDL SKLSSKPGLD LPEEELAQYD AMCPELSCSF 370 380 390 400 410 420 EELEPKCGDD LNNKDTLHAN HHHIPSVASL RQHCFNREHS SWRQEREDTV HSSILHMVKT 430 440 450 460 470 480 GKSGVPSSSK QRSATNVNQS LQQNGLEIDS SGHDTSDIQA PLEQAAWDME AISCPRITAS 490 500 510 520 530 540 FPNSTKPEES IGAAEKLLHT HAKHIPFHDP HLYIANAMRT RSAVGFKTMA FPDLWGHCPP 550 560 570 580 590 600 PAAQPMLDRK LGVQRIKILE DIRRLLHPSD VINKVVFSLD EPRPLQGSIS NCLMFHSKFE 610 620 630 640 650 660 SGNLRKAIQV REFEYDLLVN ADVNSSQHQQ WFYFKVSGMR AAVPYHFNII NCEKPNSQFN 670 680 690 700 710 720 YGMQPTLYSV KEALLGRPAW IRTGSDICYY KNHYRQNAAT MDGALGKRYY TLTFAVTFPH 730 740 750 760 770 780 NEDACYLAYH YPYTYSTLMT HLEILERSID HREIYFRHDV LCQTLGGNPC PLVTITAFPE 790 800 810 820 830 840 SNSTEHLEQF RCRPYQVITA RVHPGESNAS WVMKGTLEFL VSSDPVAKLL RENFVFKIIP 850 860 870 880 890 900 MLNPDGVING NHRCSLRGED LNRQWLSPQA HLQPTIYHAK GLLHYLSSTG RGPVVFCDFH 910 920 930 940 950 960 GHSQKKNVFL YGCSMKETLW QAGCTVGESA LLEDVSYRTL PKILDKLAPA FTMNSCSFLV 970 980 990 1000 1010 1020 EKSRASTARV VVWREMGVSR SYTMESSYCG CNQGPYQGLQ FGTGELEEMG AMYCLGLLIL 1030 1040 1050 1060 1070 1080 ELKSVNCSHK LLARASSLLN ADVLEHYLQR CSSSSSNSSN RTSEVDDEPY CMEEIDYSAD 1090 1100 1110 1120 SSSDAEQNFT ELDRQIQECA LNKDEEEEEK EEGTGWRRRS VT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel subfamily of mouse cytosolic carboxypeptidases." Kalinina E., Biswas R., Berezniuk I., Hermoso A., Aviles F.X., Fricker L.D. FASEB J. 21:836-850(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Strain: C57BL/KsJ.
[2]	"A family of protein-deglutamylating enzymes associated with neurodegeneration." Rogowski K., van Dijk J., Magiera M.M., Bosc C., Deloulme J.C., Bosson A., Peris L., Gold N.D., Lacroix B., Grau M.B., Bec N., Larroque C., Desagher S., Holzer M., Andrieux A., Moutin M.J., Janke C. Cell 143:564-578(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH MYLK, MUTAGENESIS OF HIS-757 AND GLU-760.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Lung.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/KsJ.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ867033 mRNA. Translation: ABI51952.1. FN429927 mRNA. Translation: CAZ69802.1. AK052433 mRNA. Translation: BAC34986.1. AC115830 Genomic DNA. No translation available. AC165265 Genomic DNA. No translation available. AC121812 Genomic DNA. No translation available. AC123792 Genomic DNA. No translation available. AC121971 Genomic DNA. No translation available.
IPI	IPI00226518. IPI00856787. IPI00857194. IPI00973603.
RefSeq	NP_001186153.1. NM_001199224.1.
UniGene	Mm.322829.
3D structure databases
ProteinModelPortal	Q09M05.
SMR	Q09M05. Positions 97-199, 547-869.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000103066.
Protein family/group databases
MEROPS	M14.030.
PTM databases
PhosphoSite	Q09M05.
Proteomic databases
PaxDb	Q09M05.
PRIDE	Q09M05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026854; ENSMUSP00000026854; ENSMUSG00000025754. ENSMUST00000107442; ENSMUSP00000103066; ENSMUSG00000025754.
GeneID	244071.
KEGG	mmu:244071.
UCSC	uc009hxb.1. mouse. uc009hxc.1. mouse. uc012fnc.1. mouse.
Organism-specific databases
CTD	123624.
MGI	MGI:3646469. Agbl1.
Phylogenomic databases
eggNOG	COG2866.
GeneTree	ENSGT00550000074405.
HOGENOM	HOG000293310.
HOVERGEN	HBG107587.
InParanoid	Q09M05.
OrthoDB	EOG4JDH61.
Gene expression databases
ArrayExpress	Q09M05.
Bgee	Q09M05.
CleanEx	MM_AGBL1.
Genevestigator	Q09M05.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000834. Peptidase_M14. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS00132. CARBOXYPEPT_ZN_1. False negative. PS00133. CARBOXYPEPT_ZN_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	386109.
SOURCE	Search...

Entry information

Entry name

CBPC4_MOUSE

Accession

Primary (citable) accession number: Q09M05
Secondary accession number(s): Q8C768

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	May 1, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program