ID   ABAH2_ORYSI             Reviewed;         506 AA.
AC   Q09J78; A2YW59;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   14-APR-2009, entry version 18.
DE   RecName: Full=Abscisic acid 8'-hydroxylase 2;
DE            Short=ABA 8'-hydroxylase 2;
DE            EC=1.14.13.93;
DE   AltName: Full=OsABA8ox2;
DE   AltName: Full=Cytochrome P450 707A6;
GN   Name=CYP707A6; Synonyms=ABA8OX2; ORFNames=OsI_028552;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND LACK OF
RP   INDUCTION.
RC   STRAIN=cv. Pin Gaew 56;
RX   PubMed=17022939; DOI=10.1016/j.bbrc.2006.09.098;
RA   Yang S.-H., Choi D.;
RT   "Characterization of genes encoding ABA 8'-hydroxylase in ethylene-
RT   induced stem growth of deepwater rice (Oryza sativa L.).";
RL   Biochem. Biophys. Res. Commun. 350:685-690(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Involved in the oxidative degradation of abscisic acid.
CC   -!- CATALYTIC ACTIVITY: (+)-abscisate + NADPH + O(2) = 8'-
CC       hydroxyabscisate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Plant hormone degradation; abscisic acid degradation.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- TISSUE SPECIFICITY: In internodes and expanding leaves. Weak
CC       expression in seedlings.
CC   -!- INDUCTION: Not induced by ethylene or abscisic acid treatments,
CC       and salt or osmotic stresses.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; DQ887715; ABI64255.1; -; mRNA.
DR   EMBL; CM000133; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010295; F:(+)-abscisic acid 8'-hydroxylase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP;
KW   Oxidoreductase; Transmembrane.
FT   CHAIN         1    506       Abscisic acid 8'-hydroxylase 2.
FT                                /FTId=PRO_0000288645.
FT   TRANSMEM      3     23       Potential.
FT   COMPBIAS     45     48       Poly-Lys.
FT   COMPBIAS    260    263       Poly-Gly.
FT   METAL       437    437       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   506 AA;  56327 MW;  F29DB8BD2C3D327F CRC64;
     MAFLLFFVFV TAAVLCFVVP AFLLLCTSVQ RRRDVGQGGG RDWQKKKKLR LPPGSMGWPY
     VGETLQLYSQ DPNVFFASKQ KRYGEIFKTN LLGCPCVMLA SPEAARFVLV SQARLFKPTY
     PPSKERMIGP SALFFHQGEY HLRLRRLVQA ALAPDSLRAL VPDVDAAVAA TLAAWSGGHV
     ASTFHAMKKL SFDVGVVTIF GGRLGRRHRE ELRTNYSVVE RGYNCFPNRF PGTLYHKAIQ
     ARKRLRAILS EIVAERRARG GGGDDLLGGL MRSRDDGTAG AVALLTDDQI ADNVVGVLFA
     AQDTTASVLT WILKYLHDSP KLLEAVKAEQ MAIYVANEGG KRPLTWTQTR SMTLTHQVIL
     ESLRMASIIS FTFREAVADV EYKGFLIPKG WKVMPLFRNI HHNPDYFQDP QKFDPSRFKV
     APRPSTFLPF GSGVHACPGN ELAKLEMLVL VHRLVTAYRW EIVGASDEVE YSPFPVPRGG
     LNAKLWKQEA EEDMYMAMGT ITAAGA
//