Reviewed, UniProtKB/Swiss-Prot Q09J78 (ABAH2_ORYSI)

Names and origin

Protein names

Recommended name:
    Abscisic acid 8'-hydroxylase 2
      Short name=ABA 8'-hydroxylase 2
    EC=1.14.13.93
Alternative name(s):
    OsABA8ox2
    Cytochrome P450 707A6

Gene names

Name:	CYP707A6
Synonyms:	ABA8OX2
ORF Names:	OsI_028552

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the oxidative degradation of abscisic acid.
Catalytic activity	(+)-abscisate + NADPH + O(2) = 8'-hydroxyabscisate + NADP(+) + H(2)O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone degradation; abscisic acid degradation.
Subcellular location	Membrane; Single-pass membrane proteinPotential.
Tissue specificity	In internodes and expanding leaves. Weak expression in seedlings.
Induction	Not induced by ethylene or abscisic acid treatments, and salt or osmotic stresses.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence EAZ07320.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	(+)-abscisic acid 8'-hydroxylase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Abscisic acid 8'-hydroxylase 2		PRO_0000288645
Regions
	Transmembrane	3 – 23	21	Potential
	Compositional bias	45 – 48	4	Poly-Lys
	Compositional bias	260 – 263	4	Poly-Gly
Sites
	Metal binding	437	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09J78-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: F29DB8BD2C3D327F	FASTA	506	56,327
	10 20 30 40 50 60 MAFLLFFVFV TAAVLCFVVP AFLLLCTSVQ RRRDVGQGGG RDWQKKKKLR LPPGSMGWPY 70 80 90 100 110 120 VGETLQLYSQ DPNVFFASKQ KRYGEIFKTN LLGCPCVMLA SPEAARFVLV SQARLFKPTY 130 140 150 160 170 180 PPSKERMIGP SALFFHQGEY HLRLRRLVQA ALAPDSLRAL VPDVDAAVAA TLAAWSGGHV 190 200 210 220 230 240 ASTFHAMKKL SFDVGVVTIF GGRLGRRHRE ELRTNYSVVE RGYNCFPNRF PGTLYHKAIQ 250 260 270 280 290 300 ARKRLRAILS EIVAERRARG GGGDDLLGGL MRSRDDGTAG AVALLTDDQI ADNVVGVLFA 310 320 330 340 350 360 AQDTTASVLT WILKYLHDSP KLLEAVKAEQ MAIYVANEGG KRPLTWTQTR SMTLTHQVIL 370 380 390 400 410 420 ESLRMASIIS FTFREAVADV EYKGFLIPKG WKVMPLFRNI HHNPDYFQDP QKFDPSRFKV 430 440 450 460 470 480 APRPSTFLPF GSGVHACPGN ELAKLEMLVL VHRLVTAYRW EIVGASDEVE YSPFPVPRGG 490 500 LNAKLWKQEA EEDMYMAMGT ITAAGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of genes encoding ABA 8'-hydroxylase in ethylene-induced stem growth of deepwater rice (Oryza sativa L.)." Yang S.-H., Choi D. Biochem. Biophys. Res. Commun. 350:685-690(2006) [PubMed: 17022939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, LACK OF INDUCTION. Strain: cv. Pin Gaew 56.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ887715 mRNA. Translation: ABI64255.1. CM000133 Genomic DNA. Translation: EAZ07320.1. Sequence problems.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABAH2_ORYSI

Accession

Primary (citable) accession number: Q09J78
Secondary accession number(s): A2YW59

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 17, 2006
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)