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Protein

Beta-galactosidase BgaP

Gene

bgaP

Organism
Planococcus sp. (strain L4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes lactose, o-nitrophenyl-beta-D-galactopyranoside (ONPG), p-nitrophenyl-beta-D-galactopyranoside (PNPG), 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), o-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-mannoside, o-nitrophenyl-beta-D-glucoside, p-nitrophenyl-beta-D-xyloside, p-nitrophenyl-beta-D-cellobioside, p-nitrophenyl-beta-D-arabinoside, p-nitrophenyl-beta-D-lactoside, p-nitrophenyl-beta-D-galacturonide, p-nitrophenyl-beta-D-glucuronide and p-nitrophenyl-alpha-D-galactoside with highest level of activity with ONPG as substrate, intermediate level of activity with PNPG and lower levels of activity with all other chromogenic nitrophenyl analogs. Able to hydrolyze 34% of milk lactose after 60 minutes at 5 degrees Celsius.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

No activity lost during treatment with 100 mM EDTA after 2 hours, and the addition of 1 mM MgCl2, 1 mM CaCl2 or 1 mM MnCl2 has no effect. However, the enzyme activity is inhibited by Zn2+, Cu2+, Ni2+ and Co2+ to different extents. Addition of Na+ or K+ slightly stimulates the enzyme activity at low concentrations and the optimal concentration is 250 mM. A further increase of their concentration of ions above the optimum value results in a decrease in enzyme activity. The enzyme is still active even in the presence of Na+ or K+ at a concentration up to 5 M.1 Publication

Kineticsi

  1. KM=5.4 mM for ONPG (at 5 degrees Celsius and at pH 6.8)1 Publication
  2. KM=3.8 mM for ONPG (at 10 degrees Celsius and at pH 6.8)1 Publication
  3. KM=2.9 mM for ONPG (at 20 degrees Celsius and at pH 6.8)1 Publication
  4. KM=20.4 mM for lactose (at 5 degrees Celsius and at pH 6.8)1 Publication
  5. KM=11.2 mM for lactose (at 10 degrees Celsius and at pH 6.8)1 Publication
  6. KM=10.4 mM for lactose (at 20 degrees Celsius and at pH 6.8)1 Publication

    pH dependencei

    Optimum pH is 6.8. Exhibits above 80% of its maximal activity in the pH range 6.0-8.0 using ONPG as substrate at 20 degrees Celsius.1 Publication

    Temperature dependencei

    Optimum temperature is 20 degrees Celsius. Lowering or raising the temperature from 20 degrees Celsius results in reduction of activity when ONPG is used as substrate. Rather stable at or below 25 degrees Celsius, but loses 58% of activity after 30 minutes at 40 degrees Celsius. Loses all activity in only 10 minutes at 45 degrees Celsius. Exhibits 27% of maximal activity even at 0 degrees Celsius, but enzyme activity decreases with a further increase in temperature until it is undetectable above 50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei112 – 1121SubstrateBy similarity
    Metal bindingi116 – 1161ZincBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Active sitei151 – 1511Proton donorBy similarity
    Metal bindingi156 – 1561ZincBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Metal bindingi161 – 1611ZincBy similarity
    Active sitei308 – 3081NucleophileBy similarity
    Binding sitei316 – 3161SubstrateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaP (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:bgaP1 Publication
    Synonyms:galP1 Publication
    OrganismiPlanococcus sp. (strain L4)
    Taxonomic identifieri377621 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanococcus

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Biotechnological usei

    Could conceivably be developed to fulfill the practical requirements to enable its use for lactose removal in milk and dairy products at low temperature or a reporter enzyme for psychrophilic genetic systems.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 676676Beta-galactosidase BgaPPRO_0000407693Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ09HN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni356 – 3594Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q09HN2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MINDKLPKIW HGGDYNPEQW DSQEIWDEDV RMFKLAGIDV ATLNVFSWAL
    60 70 80 90 100
    NQPNEDTYNF EWLDDKINRL YENGIYTCLA TSTAAHPAWM AKKYPDVLRV
    110 120 130 140 150
    DFYGRKRKFG SRHNSCPNSP TYREYSEKIA DKLAERYKDH PAVLIWHVSN
    160 170 180 190 200
    EYGGYCYCDN CQDAFRVWLS DKYGTLEKLN KAWNTGFWGH TFYEWDEIVA
    210 220 230 240 250
    PNMLSEERED NVSDFQGISL DYRRFQSDSL LDCYKLEYNA IRKHTPNIPI
    260 270 280 290 300
    TTNLMGTYPM LDYFKWAKEM DVVSWDNYPA IDTPFSYTAM THDLMRGLKS
    310 320 330 340 350
    GQPFMLMEQT PSQQNWQPYN SLKRPGVMRL WSYQAIGRGA DTILYFQLRR
    360 370 380 390 400
    SVGACEKYHG AVIEHVGHEH TRVFNEVAQI GKEFNQLGDT LLDARVNARV
    410 420 430 440 450
    AIVFDWENRW ATELSSGPSV SLDYVNEVHK YYDALYKLNV QVDMVGVEED
    460 470 480 490 500
    LSQYDVVIAP VLYMVKEGYA AKVESFVENG GTFITTFFSG IVNETDIVTL
    510 520 530 540 550
    GGYPGELRKV LGIWAEEIDA LHPDETNEIV VNGSRGSLSG SYSCNLLFDL
    560 570 580 590 600
    IHTEGAQAVA EYGSDFYQGM PVLTVNEFGK GKAWYVASSP DAEFLVDFLQ
    610 620 630 640 650
    TVCEEAGVEP LLSVPEGVET TERVKDGQTY LFVLNHNNKV ESIDLKDSQY
    660 670
    QELLSTQQLS GTVELEAKGV FILAKV
    Length:676
    Mass (Da):77,312
    Last modified:October 17, 2006 - v1
    Checksum:i6A2354F4544A752E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ899950 Genomic DNA. Translation: ABI64125.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ899950 Genomic DNA. Translation: ABI64125.1.

    3D structure databases

    ProteinModelPortaliQ09HN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular cloning and characterization of the gene encoding cold-active beta-galactosidase from a psychrotrophic and halotolerant Planococcus sp. L4."
      Hu J.M., Li H., Cao L.X., Wu P.C., Zhang C.T., Sang S.L., Zhang X.Y., Chen M.J., Lu J.Q., Liu Y.H.
      J. Agric. Food Chem. 55:2217-2224(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBUNIT, BIOTECHNOLOGY.
      Strain: L4Imported.

    Entry informationi

    Entry nameiBGAL_PLASL
    AccessioniPrimary (citable) accession number: Q09HN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.