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Q09HN2

- BGAL_PLASL

UniProt

Q09HN2 - BGAL_PLASL

Protein

Beta-galactosidase BgaP

Gene

bgaP

Organism
Planococcus sp. (strain L4)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Hydrolyzes lactose, o-nitrophenyl-beta-D-galactopyranoside (ONPG), p-nitrophenyl-beta-D-galactopyranoside (PNPG), 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), o-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-mannoside, o-nitrophenyl-beta-D-glucoside, p-nitrophenyl-beta-D-xyloside, p-nitrophenyl-beta-D-cellobioside, p-nitrophenyl-beta-D-arabinoside, p-nitrophenyl-beta-D-lactoside, p-nitrophenyl-beta-D-galacturonide, p-nitrophenyl-beta-D-glucuronide and p-nitrophenyl-alpha-D-galactoside with highest level of activity with ONPG as substrate, intermediate level of activity with PNPG and lower levels of activity with all other chromogenic nitrophenyl analogs. Able to hydrolyze 34% of milk lactose after 60 minutes at 5 degrees Celsius.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Enzyme regulationi

    No activity lost during treatment with 100 mM EDTA after 2 hours, and the addition of 1 mM MgCl2, 1 mM CaCl2 or 1 mM MnCl2 has no effect. However, the enzyme activity is inhibited by Zn2+, Cu2+, Ni2+ and Co2+ to different extents. Addition of Na+ or K+ slightly stimulates the enzyme activity at low concentrations and the optimal concentration is 250 mM. A further increase of their concentration of ions above the optimum value results in a decrease in enzyme activity. The enzyme is still active even in the presence of Na+ or K+ at a concentration up to 5 M.1 Publication

    Kineticsi

    1. KM=5.4 mM for ONPG (at 5 degrees Celsius and at pH 6.8)1 Publication
    2. KM=3.8 mM for ONPG (at 10 degrees Celsius and at pH 6.8)1 Publication
    3. KM=2.9 mM for ONPG (at 20 degrees Celsius and at pH 6.8)1 Publication
    4. KM=20.4 mM for lactose (at 5 degrees Celsius and at pH 6.8)1 Publication
    5. KM=11.2 mM for lactose (at 10 degrees Celsius and at pH 6.8)1 Publication
    6. KM=10.4 mM for lactose (at 20 degrees Celsius and at pH 6.8)1 Publication

    pH dependencei

    Optimum pH is 6.8. Exhibits above 80% of its maximal activity in the pH range 6.0-8.0 using ONPG as substrate at 20 degrees Celsius.1 Publication

    Temperature dependencei

    Optimum temperature is 20 degrees Celsius. Lowering or raising the temperature from 20 degrees Celsius results in reduction of activity when ONPG is used as substrate. Rather stable at or below 25 degrees Celsius, but loses 58% of activity after 30 minutes at 40 degrees Celsius. Loses all activity in only 10 minutes at 45 degrees Celsius. Exhibits 27% of maximal activity even at 0 degrees Celsius, but enzyme activity decreases with a further increase in temperature until it is undetectable above 50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei112 – 1121SubstrateBy similarity
    Metal bindingi116 – 1161ZincBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Active sitei151 – 1511Proton donorBy similarity
    Metal bindingi156 – 1561ZincBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Metal bindingi161 – 1611ZincBy similarity
    Active sitei308 – 3081NucleophileBy similarity
    Binding sitei316 – 3161SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaP (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:bgaP1 Publication
    Synonyms:galP1 Publication
    OrganismiPlanococcus sp. (strain L4)
    Taxonomic identifieri377621 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanococcus

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    Pathology & Biotechi

    Biotechnological usei

    Could conceivably be developed to fulfill the practical requirements to enable its use for lactose removal in milk and dairy products at low temperature or a reporter enzyme for psychrophilic genetic systems.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 676676Beta-galactosidase BgaPPRO_0000407693Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ09HN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni356 – 3594Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q09HN2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINDKLPKIW HGGDYNPEQW DSQEIWDEDV RMFKLAGIDV ATLNVFSWAL    50
    NQPNEDTYNF EWLDDKINRL YENGIYTCLA TSTAAHPAWM AKKYPDVLRV 100
    DFYGRKRKFG SRHNSCPNSP TYREYSEKIA DKLAERYKDH PAVLIWHVSN 150
    EYGGYCYCDN CQDAFRVWLS DKYGTLEKLN KAWNTGFWGH TFYEWDEIVA 200
    PNMLSEERED NVSDFQGISL DYRRFQSDSL LDCYKLEYNA IRKHTPNIPI 250
    TTNLMGTYPM LDYFKWAKEM DVVSWDNYPA IDTPFSYTAM THDLMRGLKS 300
    GQPFMLMEQT PSQQNWQPYN SLKRPGVMRL WSYQAIGRGA DTILYFQLRR 350
    SVGACEKYHG AVIEHVGHEH TRVFNEVAQI GKEFNQLGDT LLDARVNARV 400
    AIVFDWENRW ATELSSGPSV SLDYVNEVHK YYDALYKLNV QVDMVGVEED 450
    LSQYDVVIAP VLYMVKEGYA AKVESFVENG GTFITTFFSG IVNETDIVTL 500
    GGYPGELRKV LGIWAEEIDA LHPDETNEIV VNGSRGSLSG SYSCNLLFDL 550
    IHTEGAQAVA EYGSDFYQGM PVLTVNEFGK GKAWYVASSP DAEFLVDFLQ 600
    TVCEEAGVEP LLSVPEGVET TERVKDGQTY LFVLNHNNKV ESIDLKDSQY 650
    QELLSTQQLS GTVELEAKGV FILAKV 676
    Length:676
    Mass (Da):77,312
    Last modified:October 17, 2006 - v1
    Checksum:i6A2354F4544A752E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ899950 Genomic DNA. Translation: ABI64125.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ899950 Genomic DNA. Translation: ABI64125.1 .

    3D structure databases

    ProteinModelPortali Q09HN2.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the gene encoding cold-active beta-galactosidase from a psychrotrophic and halotolerant Planococcus sp. L4."
      Hu J.M., Li H., Cao L.X., Wu P.C., Zhang C.T., Sang S.L., Zhang X.Y., Chen M.J., Lu J.Q., Liu Y.H.
      J. Agric. Food Chem. 55:2217-2224(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBUNIT, BIOTECHNOLOGY.
      Strain: L4Imported.

    Entry informationi

    Entry nameiBGAL_PLASL
    AccessioniPrimary (citable) accession number: Q09HN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3