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Protein

Leucine--tRNA ligase

Gene

lrs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei721 – 7211ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: GO_Central

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:lrs-1
ORF Names:R74.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiR74.1; CE16317; WBGene00003073; lars-1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11861186Leucine--tRNA ligasePRO_0000152152Add
BLAST

Proteomic databases

PaxDbiQ09996.

Interactioni

Protein-protein interaction databases

BioGridi40775. 2 interactions.
IntActiQ09996. 1 interaction.
STRINGi6239.R74.1.1.

Structurei

3D structure databases

ProteinModelPortaliQ09996.
SMRiQ09996. Positions 14-944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi718 – 7225"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000012163.
HOGENOMiHOG000216621.
InParanoidiQ09996.
KOiK01869.
OMAiNIAVRTT.
PhylomeDBiQ09996.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09996-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKINKERKK VAQLQEIEKS IQELWESKKA FEADARDDGK PKYLVTFPFP
60 70 80 90 100
YMNGRLHLGH TFSASKCEFA AGFQRLQGKE VLFPFGFHCT GMPIKACADK
110 120 130 140 150
LKREMQDFGY PPNFPEDVVE EVKEEVSAVD EIIKDKSKGK KSKLVAKTGN
160 170 180 190 200
AKYQWQIMKS LGLCDEEIKE FSDPNHWLYY FPPHCIADLK KMGLKADWRR
210 220 230 240 250
SFITTDVNPY FDSFVRWQFN LLRAAKKIDF GKRYTIYSPK DGQPCMDHDR
260 270 280 290 300
ASGEGVGPQE YTLIKLKVLD PKPQALSHIK EDIYLVAATL RPETMYGQTN
310 320 330 340 350
CYLHPDIQYS VFYATENEKQ VFVATARSAR IMSYQGLTKE NGKVNYVLGL
360 370 380 390 400
EKIAGSKILG APLSAPLAKY ERVYALPMLT IKDDKGTGVV TSVPSDSPDD
410 420 430 440 450
FAALSDLKKK KPLREKYGLT DEMVLPFEPV PIIKIEGLGD LAAVEMCSRL
460 470 480 490 500
KIESQNEKDK LEEAKKEVYL KGFYDGVMLV GKYAGKKTAD VKKVIQDDLT
510 520 530 540 550
AEGLATKYVE PEKKVMSRSG DECVVALCDQ WYLNYGEAEW KAAAKKVLEP
560 570 580 590 600
LRTFNDETRR GLETTVDWLH EYACSRSYGL GTKLPWDTQY LIESLSDSTI
610 620 630 640 650
YNAYYTVAHL LQQGAFDGSV VGPAGIKADQ MTDASWSYVF LGEIYDSKTM
660 670 680 690 700
PVEEEKLKSL RKEFMYWYPI DMRASGKDLI GNHLTYLLFN HAAIWPTDTS
710 720 730 740 750
KWPKGIRANG HLLLNNEKMS KSTGNFMTLE EAIEKFSADG MRLSLADAGD
760 770 780 790 800
GLEDANFVYA MADAAILRLF TMIEWIKEMI EQRDAGLLRK DAKKFADRVF
810 820 830 840 850
ANEMNSLIAA TEQNYEATNF KDALKTGFFE YQAIRDTYRE LCAGIDEPMS
860 870 880 890 900
ESLVFRFIET QMLILSPICP HIAEYIWQLL GKDGLIVNAP WPTVDPVDEK
910 920 930 940 950
LAIGARFITE SLAEFRARLK TYMTPKKKAL KEIPEVPTEA VIYVAKEYPP
960 970 980 990 1000
WQKTILDILE KQAKANNGAL PDNKAISQLI GKEESLKKFA KKAMPFVQMI
1010 1020 1030 1040 1050
KERFEQKGVS ALASSSPVDQ TSILNENIDF IMNALDLDRV TIRHTDEEGI
1060 1070 1080 1090 1100
DANIVETTVP LVPMLNFTPN RPTIKLVARN VQICNAMFDV DVPIVNGDSV
1110 1120 1130 1140 1150
SMVIRKMRRI SKAIKPKFEV SLWRYKNAVW GDRQMISYRN PFEENIQLSD
1160 1170 1180
ADIFNFEADN KISVTSGSEK FDLGRTIVYK ANVPEN
Length:1,186
Mass (Da):134,520
Last modified:May 30, 2000 - v2
Checksum:i7CC479B71AB53979
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36238, Z46787 Genomic DNA. Translation: CAA85280.1.
PIRiT19334.
RefSeqiNP_497837.1. NM_065436.5.
UniGeneiCel.34027.

Genome annotation databases

EnsemblMetazoaiR74.1.1; R74.1.1; WBGene00003073.
R74.1.2; R74.1.2; WBGene00003073.
GeneIDi175538.
KEGGicel:CELE_R74.1.
UCSCiR74.1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36238, Z46787 Genomic DNA. Translation: CAA85280.1.
PIRiT19334.
RefSeqiNP_497837.1. NM_065436.5.
UniGeneiCel.34027.

3D structure databases

ProteinModelPortaliQ09996.
SMRiQ09996. Positions 14-944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40775. 2 interactions.
IntActiQ09996. 1 interaction.
STRINGi6239.R74.1.1.

Proteomic databases

PaxDbiQ09996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR74.1.1; R74.1.1; WBGene00003073.
R74.1.2; R74.1.2; WBGene00003073.
GeneIDi175538.
KEGGicel:CELE_R74.1.
UCSCiR74.1.1. c. elegans.

Organism-specific databases

CTDi175538.
WormBaseiR74.1; CE16317; WBGene00003073; lars-1.

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000012163.
HOGENOMiHOG000216621.
InParanoidiQ09996.
KOiK01869.
OMAiNIAVRTT.
PhylomeDBiQ09996.

Miscellaneous databases

NextBioi888576.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiSYLC_CAEEL
AccessioniPrimary (citable) accession number: Q09996
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: February 4, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.