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Protein

Leucine--tRNA ligase

Gene

lars-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in protein synthesis (PubMed:23076791). Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.Curated1 Publication

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei721ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • leucyl-tRNA aminoacylation Source: GO_Central
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • translation Source: UniProtKB
  • valyl-tRNA aminoacylation Source: GO_Central

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:lars-1Imported
Synonyms:lrs-1Imported
ORF Names:R74.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiR74.1 ; CE16317 ; WBGene00003073 ; lars-1

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in an increase in the expression of gpdh-1 independent of hypertonic stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521521 – 1186Leucine--tRNA ligaseAdd BLAST1186

Proteomic databases

EPDiQ09996
PaxDbiQ09996
PeptideAtlasiQ09996
PRIDEiQ09996

Expressioni

Gene expression databases

BgeeiWBGene00003073

Interactioni

Protein-protein interaction databases

BioGridi40775, 2 interactors
IntActiQ09996, 1 interactor
STRINGi6239.R74.1.2

Structurei

3D structure databases

ProteinModelPortaliQ09996
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 60"HIGH" regionAdd BLAST11
Motifi718 – 722"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0437 Eukaryota
COG0495 LUCA
GeneTreeiENSGT00390000012163
HOGENOMiHOG000216621
InParanoidiQ09996
KOiK01869
OMAiHVTGTPI
OrthoDBiEOG091G01VH
PhylomeDBiQ09996

Family and domain databases

InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR004493 Leu-tRNA-synth_Ia_arc/euk
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR11946:SF51 PTHR11946:SF51, 1 hit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 2 hits
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 2 hits
TIGRFAMsiTIGR00395 leuS_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q09996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKINKERKK VAQLQEIEKS IQELWESKKA FEADARDDGK PKYLVTFPFP
60 70 80 90 100
YMNGRLHLGH TFSASKCEFA AGFQRLQGKE VLFPFGFHCT GMPIKACADK
110 120 130 140 150
LKREMQDFGY PPNFPEDVVE EVKEEVSAVD EIIKDKSKGK KSKLVAKTGN
160 170 180 190 200
AKYQWQIMKS LGLCDEEIKE FSDPNHWLYY FPPHCIADLK KMGLKADWRR
210 220 230 240 250
SFITTDVNPY FDSFVRWQFN LLRAAKKIDF GKRYTIYSPK DGQPCMDHDR
260 270 280 290 300
ASGEGVGPQE YTLIKLKVLD PKPQALSHIK EDIYLVAATL RPETMYGQTN
310 320 330 340 350
CYLHPDIQYS VFYATENEKQ VFVATARSAR IMSYQGLTKE NGKVNYVLGL
360 370 380 390 400
EKIAGSKILG APLSAPLAKY ERVYALPMLT IKDDKGTGVV TSVPSDSPDD
410 420 430 440 450
FAALSDLKKK KPLREKYGLT DEMVLPFEPV PIIKIEGLGD LAAVEMCSRL
460 470 480 490 500
KIESQNEKDK LEEAKKEVYL KGFYDGVMLV GKYAGKKTAD VKKVIQDDLT
510 520 530 540 550
AEGLATKYVE PEKKVMSRSG DECVVALCDQ WYLNYGEAEW KAAAKKVLEP
560 570 580 590 600
LRTFNDETRR GLETTVDWLH EYACSRSYGL GTKLPWDTQY LIESLSDSTI
610 620 630 640 650
YNAYYTVAHL LQQGAFDGSV VGPAGIKADQ MTDASWSYVF LGEIYDSKTM
660 670 680 690 700
PVEEEKLKSL RKEFMYWYPI DMRASGKDLI GNHLTYLLFN HAAIWPTDTS
710 720 730 740 750
KWPKGIRANG HLLLNNEKMS KSTGNFMTLE EAIEKFSADG MRLSLADAGD
760 770 780 790 800
GLEDANFVYA MADAAILRLF TMIEWIKEMI EQRDAGLLRK DAKKFADRVF
810 820 830 840 850
ANEMNSLIAA TEQNYEATNF KDALKTGFFE YQAIRDTYRE LCAGIDEPMS
860 870 880 890 900
ESLVFRFIET QMLILSPICP HIAEYIWQLL GKDGLIVNAP WPTVDPVDEK
910 920 930 940 950
LAIGARFITE SLAEFRARLK TYMTPKKKAL KEIPEVPTEA VIYVAKEYPP
960 970 980 990 1000
WQKTILDILE KQAKANNGAL PDNKAISQLI GKEESLKKFA KKAMPFVQMI
1010 1020 1030 1040 1050
KERFEQKGVS ALASSSPVDQ TSILNENIDF IMNALDLDRV TIRHTDEEGI
1060 1070 1080 1090 1100
DANIVETTVP LVPMLNFTPN RPTIKLVARN VQICNAMFDV DVPIVNGDSV
1110 1120 1130 1140 1150
SMVIRKMRRI SKAIKPKFEV SLWRYKNAVW GDRQMISYRN PFEENIQLSD
1160 1170 1180
ADIFNFEADN KISVTSGSEK FDLGRTIVYK ANVPEN
Length:1,186
Mass (Da):134,520
Last modified:May 30, 2000 - v2
Checksum:i7CC479B71AB53979
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36238, Z46787 Genomic DNA Translation: CAA85280.1
PIRiT19334
RefSeqiNP_497837.1, NM_065436.5
UniGeneiCel.34027

Genome annotation databases

EnsemblMetazoaiR74.1.1; R74.1.1; WBGene00003073
R74.1.2; R74.1.2; WBGene00003073
GeneIDi175538
KEGGicel:CELE_R74.1
UCSCiR74.1.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiSYLC_CAEEL
AccessioniPrimary (citable) accession number: Q09996
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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