Q09996 (SYLC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	lrs-1
ORF Names:	R74.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1186 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW leucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1186	1186	Leucine--tRNA ligase		PRO_0000152152
Regions
	Motif	50 – 60	11	"HIGH" region
	Motif	718 – 722	5	"KMSKS" region
Sites
	Binding site	721	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09996 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 7CC479B71AB53979	FASTA	1,186	134,520
	10 20 30 40 50 60 MSKINKERKK VAQLQEIEKS IQELWESKKA FEADARDDGK PKYLVTFPFP YMNGRLHLGH 70 80 90 100 110 120 TFSASKCEFA AGFQRLQGKE VLFPFGFHCT GMPIKACADK LKREMQDFGY PPNFPEDVVE 130 140 150 160 170 180 EVKEEVSAVD EIIKDKSKGK KSKLVAKTGN AKYQWQIMKS LGLCDEEIKE FSDPNHWLYY 190 200 210 220 230 240 FPPHCIADLK KMGLKADWRR SFITTDVNPY FDSFVRWQFN LLRAAKKIDF GKRYTIYSPK 250 260 270 280 290 300 DGQPCMDHDR ASGEGVGPQE YTLIKLKVLD PKPQALSHIK EDIYLVAATL RPETMYGQTN 310 320 330 340 350 360 CYLHPDIQYS VFYATENEKQ VFVATARSAR IMSYQGLTKE NGKVNYVLGL EKIAGSKILG 370 380 390 400 410 420 APLSAPLAKY ERVYALPMLT IKDDKGTGVV TSVPSDSPDD FAALSDLKKK KPLREKYGLT 430 440 450 460 470 480 DEMVLPFEPV PIIKIEGLGD LAAVEMCSRL KIESQNEKDK LEEAKKEVYL KGFYDGVMLV 490 500 510 520 530 540 GKYAGKKTAD VKKVIQDDLT AEGLATKYVE PEKKVMSRSG DECVVALCDQ WYLNYGEAEW 550 560 570 580 590 600 KAAAKKVLEP LRTFNDETRR GLETTVDWLH EYACSRSYGL GTKLPWDTQY LIESLSDSTI 610 620 630 640 650 660 YNAYYTVAHL LQQGAFDGSV VGPAGIKADQ MTDASWSYVF LGEIYDSKTM PVEEEKLKSL 670 680 690 700 710 720 RKEFMYWYPI DMRASGKDLI GNHLTYLLFN HAAIWPTDTS KWPKGIRANG HLLLNNEKMS 730 740 750 760 770 780 KSTGNFMTLE EAIEKFSADG MRLSLADAGD GLEDANFVYA MADAAILRLF TMIEWIKEMI 790 800 810 820 830 840 EQRDAGLLRK DAKKFADRVF ANEMNSLIAA TEQNYEATNF KDALKTGFFE YQAIRDTYRE 850 860 870 880 890 900 LCAGIDEPMS ESLVFRFIET QMLILSPICP HIAEYIWQLL GKDGLIVNAP WPTVDPVDEK 910 920 930 940 950 960 LAIGARFITE SLAEFRARLK TYMTPKKKAL KEIPEVPTEA VIYVAKEYPP WQKTILDILE 970 980 990 1000 1010 1020 KQAKANNGAL PDNKAISQLI GKEESLKKFA KKAMPFVQMI KERFEQKGVS ALASSSPVDQ 1030 1040 1050 1060 1070 1080 TSILNENIDF IMNALDLDRV TIRHTDEEGI DANIVETTVP LVPMLNFTPN RPTIKLVARN 1090 1100 1110 1120 1130 1140 VQICNAMFDV DVPIVNGDSV SMVIRKMRRI SKAIKPKFEV SLWRYKNAVW GDRQMISYRN 1150 1160 1170 1180 PFEENIQLSD ADIFNFEADN KISVTSGSEK FDLGRTIVYK ANVPEN

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36238, Z46787 Genomic DNA. Translation: CAA85280.1. Z46787, Z36238 Genomic DNA. Translation: CAA86751.1.
PIR	T19334.
RefSeq	NP_497837.1. NM_065436.4.
UniGene	Cel.34027.
3D structure databases
ProteinModelPortal	Q09996.
SMR	Q09996. Positions 49-83, 228-511.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q09996. 5 interactions.
STRING	Q09996.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R74.1.1; R74.1.1; R74.1. R74.1.2; R74.1.2; R74.1.
GeneID	175538.
KEGG	cel:R74.1.
UCSC	R74.1.1. c. elegans.
Organism-specific databases
CTD	175538.
WormBase	R74.1; CE16317; WBGene00003073; lars-1.
Phylogenomic databases
eggNOG	meNOG05140.
GeneTree	EMGT00050000002558.
HOGENOM	HBG445334.
InParanoid	Q09996.
OMA	YVAKEYP.
PhylomeDB	Q09996.
Gene expression databases
ArrayExpress	Q09996.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR004493. Leu-tRNA-synth_Ia_arc/euk. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 3 hits.
KO	K01869.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 2 hits. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00395. LeuS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	888576.

Entry information

Entry name

SYLC_CAEEL

Accession

Primary (citable) accession number: Q09996

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project