Q09969 (BNIP3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NIP3 homolog
Short name=CeBNIP3

Alternative name(s):
Daf-16/FOXO controlled germline tumor affecting

Gene names

Name:	dct-1
ORF Names:	C14F5.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Initiates apoptosis in a BH3-independent mechanism possibly by recruiting ced-3 to mitochondria and other cytoplasmic membranes. Has a role in lifespan and tumor growth. Ref.2 Ref.4 Ref.5
Subunit structure	Homodimer; via transmembrane domain. Interacts with ced-3 and ced-9. Ref.1 Ref.2
Subcellular location	Mitochondrion membrane; Single-pass membrane protein Potential Ref.2.
Post-translational modification	Ubiquitinated and degraded by the proteasome. Ref.2
Sequence similarities	Belongs to the NIP3 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from mutant phenotype Ref.2. Source: UniProtKB determination of adult lifespan Inferred from mutant phenotype Ref.4. Source: UniProtKB induction of apoptosis Inferred from direct assay Ref.1. Source: WormBase regulation of cell cycle Inferred from genetic interaction Ref.5. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from direct assay Ref.2. Source: UniProtKB
Molecular function	protease binding Inferred from physical interaction Ref.2Ref.1. Source: UniProtKB protein homodimerization activity Inferred from direct assay Ref.2. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q09969-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q09969-2)

The sequence of this isoform differs from the canonical sequence as follows:
     55-57: Missing.

Note: No experimental confirmation available.

Isoform c (identifier: Q09969-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSSFLEFAKPKM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	NIP3 homolog		PRO_0000064963
Regions
	Transmembrane	178 – 198	21	Helical; Potential
	Region	178 – 198	21	Required for initiation of apoptosis
Natural variations
	Alternative sequence	1	1	M → MSSFLEFAKPKM in isoform c.		VSP_037300
	Alternative sequence	55 – 57	3	Missing in isoform b.		VSP_037301

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D6555E5479025D2C	FASTA	210	23,577
	10 20 30 40 50 60 MLDIKRKINF ASGEKTDESV QPQQQTEQSS AQQTTPSAKA VSNPFITPLT ESTPGMSESW 70 80 90 100 110 120 VELAPSRTSL CSSVDINMVI IDEKDKDSRL SPVSIAQSPH VEFESLEQVK YKLVREMLPP 130 140 150 160 170 180 GKNTDWIWDW SSRPENTPPK TVRMVQYGSN LTTPPNSPEP ELYQYLPCES DSLFNVRVVF 190 200 210 GFLVTNIFSF VVGAAVGFAV CRKLIKHHRQ
	Isoform b [UniParc]. Checksum: B0E29D984AB50639	FASTA	207	23,301
	10 20 30 40 50 60 MLDIKRKINF ASGEKTDESV QPQQQTEQSS AQQTTPSAKA VSNPFITPLT ESTPESWVEL 70 80 90 100 110 120 APSRTSLCSS VDINMVIIDE KDKDSRLSPV SIAQSPHVEF ESLEQVKYKL VREMLPPGKN 130 140 150 160 170 180 TDWIWDWSSR PENTPPKTVR MVQYGSNLTT PPNSPEPELY QYLPCESDSL FNVRVVFGFL 190 200 VTNIFSFVVG AAVGFAVCRK LIKHHRQ
	Isoform c [UniParc]. Checksum: A915F512EA06ADFE	FASTA	221	24,843
	10 20 30 40 50 60 MSSFLEFAKP KMLDIKRKIN FASGEKTDES VQPQQQTEQS SAQQTTPSAK AVSNPFITPL 70 80 90 100 110 120 TESTPGMSES WVELAPSRTS LCSSVDINMV IIDEKDKDSR LSPVSIAQSP HVEFESLEQV 130 140 150 160 170 180 KYKLVREMLP PGKNTDWIWD WSSRPENTPP KTVRMVQYGS NLTTPPNSPE PELYQYLPCE 190 200 210 220 SDSLFNVRVV FGFLVTNIFS FVVGAAVGFA VCRKLIKHHR Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of apoptosis by a Caenorhabditis elegans BNIP3 homolog." Yasuda M., D'Sa-Eipper C., Gong X.L., Chinnadurai G. Oncogene 17:2525-2530(1998) [PubMed: 9824163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), INTERACTION WITH CED-3 AND CED-9.
[2]	"The C. elegans orthologue ceBNIP3 interacts with CED-9 and CED-3 but kills through a BH3- and caspase-independent mechanism." Cizeau J., Ray R., Chen G., Gietz R.D., Greenberg A.H. Oncogene 19:5453-5463(2000) [PubMed: 11114722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), FUNCTION, HOMODIMER, SUBCELLULAR LOCATION, INTERACTION WITH CED-3 AND CED-9, UBIQUITINATION, PROTEASOMAL DEGRADATION.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[4]	"Identification of direct DAF-16 targets controlling longevity, metabolism and diapause by chromatin immunoprecipitation." Oh S.W., Mukhopadhyay A., Dixit B.L., Raha T., Green M.R., Tissenbaum H.A. Nat. Genet. 38:251-257(2006) [PubMed: 16380712] [Abstract] Cited for: FUNCTION.
[5]	"DAF-16/FOXO targets genes that regulate tumor growth in Caenorhabditis elegans." Pinkston-Gosse J., Kenyon C. Nat. Genet. 39:1403-1409(2007) [PubMed: 17934462] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF080583 mRNA. Translation: AAC31574.1. AF133832 mRNA. Translation: AAD32265.1. FO080456 Genomic DNA. Translation: CCD63843.1. FO080456 Genomic DNA. Translation: CCD63844.1.
PIR	T43338.
RefSeq	NP_509339.3. NM_076938.3. NP_509340.1. NM_076939.2.
UniGene	Cel.11376.
3D structure databases
ProteinModelPortal	Q09969.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26299N.
MINT	MINT-1079367.
Proteomic databases
PRIDE	Q09969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C14F5.1a; C14F5.1a; C14F5.1.
GeneID	181053.
KEGG	cel:C14F5.1.
Organism-specific databases
CTD	181053.
WormBase	C14F5.1a; CE01780; WBGene00015776; dct-1. C14F5.1b; CE29172; WBGene00015776; dct-1.
Phylogenomic databases
GeneTree	EMGT00050000017355.
HOGENOM	HBG388627.
InParanoid	Q09969.
OMA	MSESWVE.
PhylomeDB	Q09969.
Gene expression databases
ArrayExpress	Q09969.
Family and domain databases
InterPro	IPR010548. BNIP3. [Graphical view]
KO	K15464.
Pfam	PF06553. BNIP3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	912192.

Entry information

Entry name

BNIP3_CAEEL

Accession

Primary (citable) accession number: Q09969
Secondary accession number(s): Q95QW5, Q9UB15

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project