ID   UBPY_CAEEL              Reviewed;        1276 AA.
AC   Q09931;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 3.
DT   27-MAR-2024, entry version 141.
DE   RecName: Full=Probable ubiquitin carboxyl-terminal hydrolase K02C4.3;
DE            EC=3.4.19.12;
DE   AltName: Full=Deubiquitinating enzyme;
DE   AltName: Full=Ubiquitin thioesterase;
DE   AltName: Full=Ubiquitin-specific-processing protease;
GN   ORFNames=K02C4.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12;
CC   -!- SIMILARITY: Belongs to the peptidase C19 family. {ECO:0000305}.
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DR   EMBL; Z47811; CAA87786.3; -; Genomic_DNA.
DR   PIR; T23236; T23236.
DR   RefSeq; NP_001309441.1; NM_001322744.1.
DR   AlphaFoldDB; Q09931; -.
DR   BioGRID; 39630; 2.
DR   STRING; 6239.K02C4.3a.1; -.
DR   MEROPS; C19.A34; -.
DR   EPD; Q09931; -.
DR   PaxDb; 6239-K02C4-3; -.
DR   PeptideAtlas; Q09931; -.
DR   EnsemblMetazoa; K02C4.3a.1; K02C4.3a.1; WBGene00010502.
DR   GeneID; 174300; -.
DR   UCSC; K02C4.3; c. elegans.
DR   AGR; WB:WBGene00010502; -.
DR   WormBase; K02C4.3a; CE51326; WBGene00010502; -.
DR   eggNOG; KOG1863; Eukaryota.
DR   GeneTree; ENSGT00940000173177; -.
DR   HOGENOM; CLU_263514_0_0_1; -.
DR   InParanoid; Q09931; -.
DR   OMA; HKYELHA; -.
DR   OrthoDB; 1423057at2759; -.
DR   PhylomeDB; Q09931; -.
DR   Reactome; R-CEL-5689880; Ub-specific processing proteases.
DR   PRO; PR:Q09931; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00010502; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR   ExpressionAtlas; Q09931; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0031647; P:regulation of protein stability; IBA:GO_Central.
DR   CDD; cd02665; Peptidase_C19I; 1.
DR   Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR001394; Peptidase_C19_UCH.
DR   InterPro; IPR018200; USP_CS.
DR   InterPro; IPR028889; USP_dom.
DR   PANTHER; PTHR24006; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR   PANTHER; PTHR24006:SF938; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE K02C4.3-RELATED; 1.
DR   Pfam; PF00443; UCH; 1.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   PROSITE; PS00972; USP_1; 1.
DR   PROSITE; PS00973; USP_2; 1.
DR   PROSITE; PS50235; USP_3; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Thiol protease;
KW   Ubl conjugation pathway.
FT   CHAIN           1..1276
FT                   /note="Probable ubiquitin carboxyl-terminal hydrolase
FT                   K02C4.3"
FT                   /id="PRO_0000080685"
FT   DOMAIN          168..762
FT                   /note="USP"
FT   REGION          375..402
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        375..392
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        177
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10092,
FT                   ECO:0000255|PROSITE-ProRule:PRU10093"
FT   ACT_SITE        707
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10092,
FT                   ECO:0000255|PROSITE-ProRule:PRU10093"
SQ   SEQUENCE   1276 AA;  144893 MW;  EB5293F0045028D4 CRC64;
     MVEENETGTP GTSRTVTFHD GRKLTDAEHF VFFKVKEVIA DKVAEAEILE SIRKRSECKP
     TDEQFISDII NELFYSGEPP KGRKSERFIG PLFDPEKAST ASGPMDCTDV VSYDSTHPNI
     SEISKKEEVE MQSAIQQSLA SSASQNISRP TMLMSNLEDM VRNPNFSTGL YNSGNTCWLN
     CLSQVLYSIP KFRSILYHCA PLSWHEQPIT NVKIENQQHA ELLMLFRGLF AELQFSEMKY
     IEVGPLINMV DKLSKSSKGP STIGTQQDAT EMLTLIFDWL QRAFDAALHA QLNPEFSNVS
     DEENLVISDS TTTAPNSDII GAPPGYNAAN LSLPSSSHVD PKSTLNPMYV NEKEPSSTPT
     SLFGTRSKTI EVNESMDTEA ATSSNLPGNS VENHPNPAAP EVDDNKKAFC DKLKESFNNI
     FSSVCYTESV AEDGTVSVKS NVRNCPQFFQ LQVTYGNLHD ALEAATFDHG LGNTASHVRN
     LYDPLPAVIF FGLSRFSFNS NIESKLHDKF TFPKIIFMDR YLKCNKEQLV QLRSHRELCR
     DSLSEVRAKL SGLRRYPQGN GEVRLEDSFQ TVWQAVSNFR EFVTFYLKVS QKTFFSREDA
     HENTAFVGPL TPSTYQSSSD NCSSKFVKDG GKLFPTFTEG FFPGKAAFIE TLQNMLEALK
     TEERDCLAEE ARLQEVIDQT YEVPELQQHK YELHAIIVHS GEANRGHYWT YKLKKSIDGL
     EEWEKLNDQN ADRVDWPKVE SDSFGTGSRD APSAYMLMYV RSDAEWLVSA DKLTALEAFE
     TIPPDLQEKV LQKRDEFKEK LQRFRENKEF NYQQFSVDSP TVQSTEETPS SFSWYRDELE
     DIDIGDENAN PTKNDYLLNA RLDSYSVPIA PDVETSEMKR MVSQMWNQIT KIAPRKYTDS
     QDLLDSNLRS VMEGESGGIN FINSRLGYDI HELRSDADND VEGVYNAFIN EYLGLVKDLH
     ELQNSKFVVF VGFQLQRIHV PVLRYLLVRA MAVSELGIIS QRANNELSGM SSNSHDKGTA
     MLQIALLLSH FFELGVMSAW GCRSSLENIH VILNDFKKKN SRGSEQIEVT YCAMIGARNA
     RICNGLLREM AYFLESYSIF FVSQKHIEAC TVFSTITMIK LIMQHMASKT LQLIDMEFHL
     SKNERRVRFE DIIREVVSSV CIIHHWSKSY SKEFQNEINL KELMGLLHLK VEFMVSLTSF
     EVADPKYECL QAFKAMVVNT VMDLERASNE LDYDVLDGAV ELRELKKYFK ELQLTDVKVN
     NIITSYDPII ESLVKI
//