Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q09925

- LCB2_SCHPO

UniProt

Q09925 - LCB2_SCHPO

Protein

Serine palmitoyltransferase 2

Gene

lcb2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine.By similarity

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: PomBase

    GO - Biological processi

    1. sphingolipid biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_188653. Sphingolipid de novo biosynthesis.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 2 (EC:2.3.1.50)
    Short name:
    SPT 2
    Alternative name(s):
    Long chain base biosynthesis protein 2
    Gene namesi
    Name:lcb2
    ORF Names:SPAC21E11.08, SPAC2C4.02
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC21E11.08.

    Subcellular locationi

    Cytoplasm By similarity. Endoplasmic reticulum 1 Publication. Membrane Curated; Single-pass membrane protein Curated

    GO - Cellular componenti

    1. endoplasmic reticulum Source: PomBase
    2. integral component of membrane Source: UniProtKB-KW
    3. serine C-palmitoyltransferase complex Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 603603Serine palmitoyltransferase 2PRO_0000163861Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei398 – 3981N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiQ09925.
    PRIDEiQ09925.

    Interactioni

    Subunit structurei

    Lcb1 and lcb2 encode essential subunits of the enzyme and form a heterodimer.By similarity

    Protein-protein interaction databases

    BioGridi280491. 5 interactions.
    MINTiMINT-4696465.
    STRINGi4896.SPAC21E11.08-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09925.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei90 – 10718HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0156.
    HOGENOMiHOG000206826.
    KOiK00654.
    OMAiPMYVAVM.
    OrthoDBiEOG7XPZG0.
    PhylomeDBiQ09925.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q09925-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQADFVSPT SIDVSEKKEV EFHKKVDHVE NPPLSTESAK LEAEEVAAEK    50
    LNSEHLLENE FAPITDPTHR RVSKNPDGAE LFQFEDEPSY YYVVATYLTY 100
    LVLIIIGHVR DFFGKRFHKD DYKYLKDNDG YAPLYNHFDN FYVRRLQHRI 150
    NDCFSRPTMG VPGRVIRLMN RYSTDSNSTF KLTGDTSLAL NVSSYNYLGF 200
    AQSHGPCATK VEEAMQKYGL STCSSNAICG TYGLHKEVEE LTANFVGKPA 250
    ALVFSQGFST NATVFSTLMC PGSLIISDEL NHTSIRFGAR LSGANIRVYK 300
    HNDMTDLERV LREVISQGQP RTHRPYSKIL VVIEGLYSME GNFCDLPKVV 350
    ELKNRYKFYL FIDEAHSIGA IGPRGGGICD YFGISTDHVD ILMGTFTKSF 400
    GAAGGYISAT PNIINKLRVT NPGYVYAESM SPAVLAQIKS SFLEIMDNSP 450
    TSAGLERIER LAFNSRYIRL GLKRLGFIIF GNDDSPVVPL LLYNPGKINA 500
    FSHEMLKRGI AVVVVGYPAC PLLTSRVRFC FSASHNKADM DYFLRACDEV 550
    GEKLQLKFST GAAGEDVGKT NVEKMKKNQG WFKPPRWKIE DVLKHGVHDA 600
    LTQ 603
    Length:603
    Mass (Da):67,519
    Last modified:February 1, 1996 - v1
    Checksum:i251A2383CC9B8472
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15645 Genomic DNA. Translation: AAC49534.1.
    CU329670 Genomic DNA. Translation: CAA91967.2.
    PIRiJC5183.
    RefSeqiXP_001713103.1. XM_001713051.2.

    Genome annotation databases

    EnsemblFungiiSPAC21E11.08.1; SPAC21E11.08.1:pep; SPAC21E11.08.
    GeneIDi3361415.
    KEGGispo:SPAC21E11.08.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15645 Genomic DNA. Translation: AAC49534.1 .
    CU329670 Genomic DNA. Translation: CAA91967.2 .
    PIRi JC5183.
    RefSeqi XP_001713103.1. XM_001713051.2.

    3D structure databases

    ProteinModelPortali Q09925.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 280491. 5 interactions.
    MINTi MINT-4696465.
    STRINGi 4896.SPAC21E11.08-1.

    Proteomic databases

    MaxQBi Q09925.
    PRIDEi Q09925.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC21E11.08.1 ; SPAC21E11.08.1:pep ; SPAC21E11.08 .
    GeneIDi 3361415.
    KEGGi spo:SPAC21E11.08.

    Organism-specific databases

    PomBasei SPAC21E11.08.

    Phylogenomic databases

    eggNOGi COG0156.
    HOGENOMi HOG000206826.
    KOi K00654.
    OMAi PMYVAVM.
    OrthoDBi EOG7XPZG0.
    PhylomeDBi Q09925.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    Reactomei REACT_188653. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    NextBioi 20811466.
    PROi Q09925.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sphingolipid synthesis: identification and characterization of mammalian cDNAs encoding the Lcb2 subunit of serine palmitoyltransferase."
      Nagiec M.M., Lester R.L., Dickson R.C.
      Gene 177:237-241(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLCB2_SCHPO
    AccessioniPrimary (citable) accession number: Q09925
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3