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Protein

ATP-dependent RNA helicase has1

Gene

has1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 140ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase has1 (EC:3.6.4.13)
Gene namesi
Name:has1
ORF Names:SPAC1F7.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1F7.02c
PomBaseiSPAC1F7.02c has1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550871 – 578ATP-dependent RNA helicase has1Add BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60Phosphoserine1 Publication1
Modified residuei62Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09916
PaxDbiQ09916
PRIDEiQ09916

PTM databases

iPTMnetiQ09916

Interactioni

Subunit structurei

Associates in the nucleolus with the 60S and pre-60S ribosomal subunits.By similarity

Protein-protein interaction databases

BioGridi278164, 7 interactors
STRINGi4896.SPAC1F7.02c.1

Structurei

3D structure databases

ProteinModelPortaliQ09916
SMRiQ09916
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 296Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST177
Domaini310 – 480Helicase C-terminalPROSITE-ProRule annotationAdd BLAST171

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi89 – 117Q motifAdd BLAST29
Motifi243 – 246DEAD box4
Motifi322 – 338Bipartite nuclear localization signalBy similarityAdd BLAST17

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000268799
InParanoidiQ09916
KOiK13179
OMAiEMRQIVK
OrthoDBiEOG092C1VLP
PhylomeDBiQ09916

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR025313 DUF4217
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF13959 DUF4217, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM01178 DUF4217, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q09916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSELKRKK HQSGNEEVKE KRQKPLKNDK KIAEELPQDE DDYEQEEENE
60 70 80 90 100
DADQNTSVES ESEELDNENE DERVQKSVNL NASSTSDIEK FSDLQLSENI
110 120 130 140 150
QKAIKEMGFE TMTEIQKRSI PPLLAGRDVL GAAKTGSGKT LAFLIPTIEM
160 170 180 190 200
LYALKFKPRN GTGVIIISPT RELALQIFGV AKELLKYHHQ TFGIVIGGAN
210 220 230 240 250
RRAEADKLVK GVNLLVATPG RLLDHLQNTK GFVFRNLRSL VIDEADRILE
260 270 280 290 300
IGFEDEMRQI MKILPSENRQ TLLFSATQTT KVEDLARISL KPGPLYVNVD
310 320 330 340 350
SGKPTSTVEG LEQGYVVVDS DKRFLLLFSF LKRNLKKKVI VFMSSCASVK
360 370 380 390 400
YMAELLNYID LPVLDLHGKQ KQQRRTNTFF EFCNAEKGIL LCTNVAARGL
410 420 430 440 450
DIPAVDWIVQ YDPPDDPRDY IHRVGRTARG TKGTGKSLMF LAPSELGFLR
460 470 480 490 500
YLKTAKVSLN EFEFPANKVA NVQSQLEKLV SKNYYLQQSA KDGYRSYLQA
510 520 530 540 550
YASYSLKSIF DINKLDLAKV AKSFGFAHPP NVNITIGASG RTDKKERRAG
560 570
YNKKNHVDVY SKQRSSAISQ DKERGWSR
Length:578
Mass (Da):65,261
Last modified:February 1, 1996 - v1
Checksum:i37E8FDB4081901E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA91949.1
PIRiS62574
RefSeqiNP_594488.1, NM_001019917.2

Genome annotation databases

EnsemblFungiiSPAC1F7.02c.1; SPAC1F7.02c.1:pep; SPAC1F7.02c
GeneIDi2541668
KEGGispo:SPAC1F7.02c

Similar proteinsi

Entry informationi

Entry nameiHAS1_SCHPO
AccessioniPrimary (citable) accession number: Q09916
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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