Q09909 (CWF19_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor cwf19

Alternative name(s):
Complexed with cdc5 protein 19

Gene names

Name:	cwf19
ORF Names:	SPAC30D11.09

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome. Involved in mRNA splicing.
Subunit structure	Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the CWF19 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA cis splicing, via spliceosome Traceable author statement. Source: GeneDB_Spombe
Cellular component	spliceosomal complex Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	Pre-mRNA-splicing factor cwf19		PRO_0000079614

Sequences

	Sequence		Length	Mass (Da)
	Q09909 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 95F79E570802DA1B	FASTA	639	74,420
	10 20 30 40 50 60 MEKEKLTNRT STHKFNETDR ETNHSRRHRH HHSRHRESKH GRHDRSERPS SREAGDRSRH 70 80 90 100 110 120 ERFSKEPLEV KKLPVENEYF DSKFASASSM PSSKDNPQNN DFTNSLQGLD FGTFGSRIQR 130 140 150 160 170 180 EPIDRKSNKP GEIFVGATLQ NESSTSTEHK DIEQLETEIN ESGINYKIGD NGSRWRMMKL 190 200 210 220 230 240 KRVFDMAQDQ RRPVEEVALE RYGSLKEFDF ALEERDELEL RKRTRKVQKE VPTGELYEKR 250 260 270 280 290 300 CADEFKTRKD SGNGATYYKV IPDPSVNKDT PTESDLNRLK ADLLRAQLKK DPNYESLERE 310 320 330 340 350 360 YQKACDDFEN SLHPSENSNS QATIPSKKRN FDDPTIDEMV QEEKLLNKQR KYGQNYEYAK 370 380 390 400 410 420 QIAKDKTYSN NLDYLDENAG KLSNRMKRGD VSLAQISSGG DLKKINHVLD TCPLCLNYET 430 440 450 460 470 480 QPLAPVISLS HRAYVSLPTQ PELAKYHCLI VPTGHRINTL SCDEDEWDEI RNFMKCIALM 490 500 510 520 530 540 FDSMNLGVIF YENAPSPQRY MHTAIECIPV SKRILSLAPA YFREALSTSD EEWSQHRKII 550 560 570 580 590 600 DTLEGSKKYG KWAFRKMMVK ELGYFHVWFS IDGGYGHVVE DEKAWGRHDQ VPRQVFASML 610 620 630 NLPPEVIRRK GSWTGKKDPR EDMFRSRFEK FDWTKGLID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed: 11884590] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWF COMPLEX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA91895.1.
PIR	S62567. T38590.
RefSeq	NP_593208.1. NM_001018604.1.
3D structure databases
ProteinModelPortal	Q09909.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q09909. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC30D11.09.1; SPAC30D11.09.1:pep; SPAC30D11.09.
GeneID	2543189.
GenomeReviews	Gene locus cwf19 in contig CU329670_GR.
KEGG	spo:SPAC30D11.09.
NMPDR	fig|4896.1.peg.3178.
Organism-specific databases
GeneDB_Spombe	SPAC30D11.09.
Phylogenomic databases
eggNOG	fuNOG06477.
GeneTree	EFGT00070000008757.
OrthoDB	EOG4F21BW.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000902-MONOMER.
Gene expression databases
ArrayExpress	Q09909.
Family and domain databases
InterPro	IPR006768. Cwf19-like_C_dom-1. IPR006767. Cwf19-like_C_dom-2. IPR011146. His_triad-like_motif. [Graphical view]
Pfam	PF04677. CwfJ_C_1. 1 hit. PF04676. CwfJ_C_2. 1 hit. [Graphical view]
SUPFAM	SSF54197. His_triad-like_motif. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CWF19_SCHPO

Accession

Primary (citable) accession number: Q09909

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program