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Protein

Mitogen-activated protein kinase sty1

Gene

sty1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Involved in osmoregulation and stress response pathways leading to an efficient start of sexual differentiation. Supports translation initiation and facilitates adaptation to environmental stress in part through reducing eIF2-alpha phosphorylation. Links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Phosphorylates atf1 and mkp1. In conjunction with hal4, has a role in the cellular resistance to toxic cations such as Na+, Li+ and Ca2+. Involved in resistance to arsenite, methylglyoxal and hydrogen peroxide. Involved in induction of thermotolerance in mRNA export, as well as in vacuolar fission.16 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by the MAPK kinase wisl, and negatively regulated by pypl and pyp2 tyrosine phosphatases.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei141Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: PomBase
  • SAP kinase activity Source: PomBase

GO - Biological processi

  • activation of reciprocal meiotic recombination Source: PomBase
  • cellular response to heat Source: PomBase
  • cellular response to nutrient Source: PomBase
  • cellular response to osmotic stress Source: PomBase
  • cellular response to oxidative stress Source: PomBase
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of induction of conjugation with cellular fusion Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of mitotic G1 cell cycle arrest in response to nitrogen starvation Source: PomBase
  • positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues Source: PomBase
  • positive regulation of protein import into nucleus Source: PomBase
  • positive regulation of recombination hotspot binding Source: PomBase
  • positive regulation of transcription by RNA polymerase II Source: PomBase
  • positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: PomBase
  • protein stabilization Source: PomBase
  • regulation of aerobic respiration Source: PomBase
  • regulation of cytoplasmic translational initiation in response to stress Source: PomBase
  • regulation of meiotic cell cycle Source: PomBase
  • regulation of reciprocal meiotic recombination Source: PomBase
  • stress-activated MAPK cascade Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Kinase, Serine/threonine-protein kinase, Transferase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 5613
ReactomeiR-SPO-168638 NOD1/2 Signaling Pathway
R-SPO-193648 NRAGE signals death through JNK
R-SPO-2559580 Oxidative Stress Induced Senescence
R-SPO-2871796 FCERI mediated MAPK activation
R-SPO-375170 CDO in myogenesis
R-SPO-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
R-SPO-450341 Activation of the AP-1 family of transcription factors
R-SPO-6798695 Neutrophil degranulation
R-SPO-9007892 Interleukin-38 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase sty1 (EC:2.7.11.24)
Short name:
MAP kinase sty1
Alternative name(s):
MAP kinase spc1
Gene namesi
Name:sty1
Synonyms:hog1, phh1, spc1
ORF Names:SPAC24B11.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24B11.06c
PomBaseiSPAC24B11.06c sty1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863421 – 349Mitogen-activated protein kinase sty1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171Phosphothreonine1 Publication1
Modified residuei173Phosphotyrosine1 Publication1
Modified residuei175Phosphoserine1 Publication1
Modified residuei176Phosphothreonine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme (By similarity). Phosphorylated by wis1 in response to osmotic stress, nutrient limitation, hydrogen peroxide and arsenite. Dephosphorylated by pyp1 and pyp2.By similarity9 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09892
PaxDbiQ09892
PRIDEiQ09892

PTM databases

iPTMnetiQ09892

Interactioni

Subunit structurei

Interacts with cdc37, cmk2, hal4, sin1 and srk1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi278148, 297 interactors
IntActiQ09892, 5 interactors
MINTiQ09892
STRINGi4896.SPAC24B11.06c.1

Structurei

3D structure databases

ProteinModelPortaliQ09892
SMRiQ09892
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST280

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 173TXY3
Motifi176 – 178TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233024
InParanoidiQ09892
KOiK04441
OMAiDVIDTIC
OrthoDBiEOG092C2FL8
PhylomeDBiQ09892

Family and domain databases

CDDicd07856 STKc_Sty1_Hog1, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR038783 MAPK_Sty1/Hog1
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24055:SF247 PTHR24055:SF247, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q09892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFIRTQIF GTCFEITTRY SDLQPIGMGA FGLVCSAKDQ LTGMNVAVKK
60 70 80 90 100
IMKPFSTPVL AKRTYRELKL LKHLRHENII SLSDIFISPF EDIYFVTELL
110 120 130 140 150
GTDLHRLLTS RPLETQFIQY FLYQILRGLK FVHSAGVIHR DLKPSNILIN
160 170 180 190 200
ENCDLKICDF GLARIQDPQM TGYVSTRYYR APEIMLTWQK YNVEVDIWSA
210 220 230 240 250
GCIFAEMIEG KPLFPGRDHV NQFSIITELL GTPPMEVIET ICSKNTLRFV
260 270 280 290 300
QSLPQKEKVP FAEKFKNADP DAIDLLEKML VFDPRKRISA ADALAHNYLA
310 320 330 340
PYHDPTDEPV ADEVFDWSFQ DNDLPVETWK VMMYSEVLSF HNMDNELQS
Length:349
Mass (Da):40,222
Last modified:February 1, 1996 - v1
Checksum:i9A10835FFD381AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89262 Genomic DNA Translation: CAA61537.1
U26739 mRNA Translation: AAA91020.1
CU329670 Genomic DNA Translation: CAA91771.1
PIRiS68675
RefSeqiNP_592843.1, NM_001018244.2

Genome annotation databases

EnsemblFungiiSPAC24B11.06c.1; SPAC24B11.06c.1:pep; SPAC24B11.06c
GeneIDi2541652
KEGGispo:SPAC24B11.06c

Similar proteinsi

Entry informationi

Entry nameiHOG1_SCHPO
AccessioniPrimary (citable) accession number: Q09892
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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