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Protein

Mitogen-activated protein kinase sty1

Gene

sty1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Involved in osmoregulation and stress response pathways leading to an efficient start of sexual differentiation. Supports translation initiation and facilitates adaptation to environmental stress in part through reducing eIF2-alpha phosphorylation. Links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Phosphorylates atf1 and mkp1. In conjunction with hal4, has a role in the cellular resistance to toxic cations such as Na+, Li+ and Ca2+. Involved in resistance to arsenite, methylglyoxal and hydrogen peroxide. Involved in induction of thermotolerance in mRNA export, as well as in vacuolar fission.16 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by the MAPK kinase wisl, and negatively regulated by pypl and pyp2 tyrosine phosphatases.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei141Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: PomBase
  • MAP kinase activity involved in conjugation with cellular fusion Source: PomBase
  • MAP kinase activity involved in osmosensory signaling pathway Source: PomBase
  • SAP kinase activity Source: PomBase

GO - Biological processi

  • cellular response to cation stress Source: PomBase
  • MAPK cascade in response to starvation Source: PomBase
  • MAPK cascade involved in osmosensory signaling pathway Source: PomBase
  • mitotic G1 cell cycle arrest in response to nitrogen starvation Source: PomBase
  • mRNA export from nucleus in response to heat stress Source: PomBase
  • negative regulation of induction of conjugation with cellular fusion by transcription from RNA polymerase II promoter Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues Source: PomBase
  • positive regulation of protein kinase activity Source: PomBase
  • positive regulation of recombination hotspot binding Source: PomBase
  • positive regulation of transcription factor import into nucleus in response to oxidative stress Source: PomBase
  • positive regulation of transcription from RNA polymerase II promoter Source: PomBase
  • positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: PomBase
  • protein phosphorylation Source: PomBase
  • regulation of cAMP-mediated signaling Source: PomBase
  • regulation of cAMP-mediated signaling by regulation of transcription from RNA polymerase II promoter Source: PomBase
  • regulation of chromatin assembly Source: PomBase
  • regulation of chromatin disassembly Source: PomBase
  • regulation of cytoplasmic translation in response to stress Source: PomBase
  • regulation of histone acetylation Source: PomBase
  • regulation of meiotic cell cycle Source: PomBase
  • regulation of mRNA stability involved in cellular response to UV Source: PomBase
  • regulation of reciprocal meiotic recombination Source: PomBase
  • stress-activated MAPK cascade Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 5613.
ReactomeiR-SPO-168638. NOD1/2 Signaling Pathway.
R-SPO-193648. NRAGE signals death through JNK.
R-SPO-2559580. Oxidative Stress Induced Senescence.
R-SPO-2871796. FCERI mediated MAPK activation.
R-SPO-375170. CDO in myogenesis.
R-SPO-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-SPO-450341. Activation of the AP-1 family of transcription factors.
R-SPO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase sty1 (EC:2.7.11.24)
Short name:
MAP kinase sty1
Alternative name(s):
MAP kinase spc1
Gene namesi
Name:sty1
Synonyms:hog1, phh1, spc1
ORF Names:SPAC24B11.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24B11.06c.
PomBaseiSPAC24B11.06c. sty1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Predominantly cytoplasmic in unstressed cells but rapidly concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation.

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrial inner membrane Source: PomBase
  • mitochondrial intermembrane space Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863421 – 349Mitogen-activated protein kinase sty1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171Phosphothreonine1 Publication1
Modified residuei173Phosphotyrosine1 Publication1
Modified residuei175Phosphoserine1 Publication1
Modified residuei176Phosphothreonine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme (By similarity). Phosphorylated by wis1 in response to osmotic stress, nutrient limitation, hydrogen peroxide and arsenite. Dephosphorylated by pyp1 and pyp2.By similarity9 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09892.
PRIDEiQ09892.

PTM databases

iPTMnetiQ09892.

Interactioni

Subunit structurei

Interacts with cdc37, cmk2, hal4, sin1 and srk1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ptc4O141564EBI-3648525,EBI-7593590
srk1O945473EBI-3648525,EBI-3648527

Protein-protein interaction databases

BioGridi278148. 297 interactors.
IntActiQ09892. 5 interactors.
MINTiMINT-4696085.

Structurei

3D structure databases

ProteinModelPortaliQ09892.
SMRiQ09892.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST280

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 173TXY3
Motifi176 – 178TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiQ09892.
KOiK04441.
OMAiLSHEYLA.
OrthoDBiEOG092C2FL8.
PhylomeDBiQ09892.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFIRTQIF GTCFEITTRY SDLQPIGMGA FGLVCSAKDQ LTGMNVAVKK
60 70 80 90 100
IMKPFSTPVL AKRTYRELKL LKHLRHENII SLSDIFISPF EDIYFVTELL
110 120 130 140 150
GTDLHRLLTS RPLETQFIQY FLYQILRGLK FVHSAGVIHR DLKPSNILIN
160 170 180 190 200
ENCDLKICDF GLARIQDPQM TGYVSTRYYR APEIMLTWQK YNVEVDIWSA
210 220 230 240 250
GCIFAEMIEG KPLFPGRDHV NQFSIITELL GTPPMEVIET ICSKNTLRFV
260 270 280 290 300
QSLPQKEKVP FAEKFKNADP DAIDLLEKML VFDPRKRISA ADALAHNYLA
310 320 330 340
PYHDPTDEPV ADEVFDWSFQ DNDLPVETWK VMMYSEVLSF HNMDNELQS
Length:349
Mass (Da):40,222
Last modified:February 1, 1996 - v1
Checksum:i9A10835FFD381AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89262 Genomic DNA. Translation: CAA61537.1.
U26739 mRNA. Translation: AAA91020.1.
CU329670 Genomic DNA. Translation: CAA91771.1.
PIRiS68675.
RefSeqiNP_592843.1. NM_001018244.2.

Genome annotation databases

EnsemblFungiiSPAC24B11.06c.1; SPAC24B11.06c.1:pep; SPAC24B11.06c.
GeneIDi2541652.
KEGGispo:SPAC24B11.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89262 Genomic DNA. Translation: CAA61537.1.
U26739 mRNA. Translation: AAA91020.1.
CU329670 Genomic DNA. Translation: CAA91771.1.
PIRiS68675.
RefSeqiNP_592843.1. NM_001018244.2.

3D structure databases

ProteinModelPortaliQ09892.
SMRiQ09892.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278148. 297 interactors.
IntActiQ09892. 5 interactors.
MINTiMINT-4696085.

PTM databases

iPTMnetiQ09892.

Proteomic databases

MaxQBiQ09892.
PRIDEiQ09892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC24B11.06c.1; SPAC24B11.06c.1:pep; SPAC24B11.06c.
GeneIDi2541652.
KEGGispo:SPAC24B11.06c.

Organism-specific databases

EuPathDBiFungiDB:SPAC24B11.06c.
PomBaseiSPAC24B11.06c. sty1.

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiQ09892.
KOiK04441.
OMAiLSHEYLA.
OrthoDBiEOG092C2FL8.
PhylomeDBiQ09892.

Enzyme and pathway databases

BRENDAi2.7.11.24. 5613.
ReactomeiR-SPO-168638. NOD1/2 Signaling Pathway.
R-SPO-193648. NRAGE signals death through JNK.
R-SPO-2559580. Oxidative Stress Induced Senescence.
R-SPO-2871796. FCERI mediated MAPK activation.
R-SPO-375170. CDO in myogenesis.
R-SPO-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-SPO-450341. Activation of the AP-1 family of transcription factors.
R-SPO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ09892.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOG1_SCHPO
AccessioniPrimary (citable) accession number: Q09892
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.