Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Dicer

Gene

dcr1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for G1 arrest and mating in response to nitrogen starvation. Ago1 regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. Required, indirectly, for regulated hyperphosphorylation of cdc2.
Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Digests double-stranded RNA (dsRNA) producing 21 to 23 bp dsRNAs, so-called interfering RNAs (siRNA). Required for both post-transcriptional and transcriptional gene silencing. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3 'Lys-10' methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat.

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1123 – 11231Magnesium or manganeseBy similarity
Sitei1215 – 12151Important for activityBy similarity
Metal bindingi1219 – 12191Magnesium or manganeseBy similarity
Metal bindingi1222 – 12221Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 398ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: PomBase
  • double-stranded DNA binding Source: PomBase
  • double-stranded RNA binding Source: PomBase
  • euchromatin binding Source: PomBase
  • helicase activity Source: UniProtKB-KW
  • ribonuclease activity Source: PomBase
  • ribonuclease III activity Source: GO_Central
  • zinc ion binding Source: PomBase

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular protein localization Source: PomBase
  • chromatin silencing at centromere outer repeat region Source: PomBase
  • chromatin silencing by small RNA Source: PomBase
  • chromosome segregation Source: UniProtKB-KW
  • co-transcriptional gene silencing by RNA interference machinery Source: PomBase
  • establishment of chromatin silencing at silent mating-type cassette Source: PomBase
  • heterochromatin organization involved in chromatin silencing Source: PomBase
  • negative regulation of gene expression Source: CACAO
  • positive regulation of termination of RNA polymerase II transcription Source: PomBase
  • production of siRNA involved in RNA interference Source: PomBase
  • regulation of chromatin silencing at centromere Source: PomBase
  • regulation of histone H3-K9 methylation Source: PomBase
  • RNA processing Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

Cell cycle, Chromosome partition, RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Dicer
Alternative name(s):
Cell cycle control protein dcr1
RNA interference pathway protein dcr1
Including the following 2 domains:
Endoribonuclease dcr1 (EC:3.1.26.-)
ATP-dependent helicase dcr1 (EC:3.6.4.-)
Gene namesi
Name:dcr1
ORF Names:SPCC188.13c, SPCC584.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC188.13c.
PomBaseiSPCC188.13c. dcr1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric outer repeat region Source: GOC
  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear euchromatin Source: PomBase
  • nuclear pericentric heterochromatin Source: PomBase
  • nuclear periphery Source: PomBase
  • nucleus Source: PomBase
  • RISC complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13741374Protein DicerPRO_0000102194Add
BLAST

PTM databases

SwissPalmiQ09884.

Interactioni

Protein-protein interaction databases

BioGridi275506. 184 interactions.
MINTiMINT-4696006.

Structurei

Secondary structure

1
1374
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1262 – 12709Combined sources
Turni1271 – 12744Combined sources
Beta strandi1278 – 12869Combined sources
Beta strandi1294 – 12974Combined sources
Beta strandi1303 – 13119Combined sources
Beta strandi1314 – 132310Combined sources
Helixi1324 – 134118Combined sources
Helixi1344 – 13474Combined sources
Turni1351 – 13533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L6MNMR-A1259-1358[»]
ProteinModelPortaliQ09884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 206188Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini340 – 517178Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini537 – 62892Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini916 – 1038123RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1083 – 1233151RNase III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi145 – 1484DECH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiQ09884.
KOiK11592.
OrthoDBiEOG092C0CUQ.
PhylomeDBiQ09884.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 2 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDISSFLLPQ LLRKYQQDVY NIASKQNTLL VMRTGAGKTL LAVKLIKQKL
60 70 80 90 100
EEQILIQESN LEHKKISVFL VNKVPLVFQQ AEYIRSQLPA KVGMFYGELS
110 120 130 140 150
IEMSEQLLTN IILKYNVIVI TADLFYLFLA RGFLSINDLN LIIFDECHHA
160 170 180 190 200
IGNDAYARIM NDFYHRAKAV LSKKHFTLPR IFGMTASPFT GKKGNLYHRL
210 220 230 240 250
YQWEQLFDSK AHVVSENELA DYFCLPEESY VMYSNKLVVP PSDSIIKKCE
260 270 280 290 300
ETLQGCKLIS RAVKTALAET IDMGLWFGEQ VWLYLVDFVE TKRLKKKALG
310 320 330 340 350
KQLSDDEELA IDRLKIFVED WKNNKYSDNG PRIPVFDSTD VTDKVFKLLE
360 370 380 390 400
LLKATYRKSD SVRTVIFVER KATAFTLSLF MKTLNLPNIR AHSFIGHGPS
410 420 430 440 450
DQGEFSMTFR RQKDTLHKFK TGKYNVLIAT AVAEEGIDVP SCNLVIRFNI
460 470 480 490 500
CRTVTQYVQS RGRARAMASK FLIFLNTEEL LIHERILHEE KNLKFALSEL
510 520 530 540 550
SNSNIFDSLV CEERERVTDD IVYEVGETGA LLTGLYAVSL LYNFCNTLSR
560 570 580 590 600
DVYTRYYPTF TAQPCLSGWY CFEVELPKAC KVPAAQGSPA KSIRKAKQNA
610 620 630 640 650
AFIMCLDLIR MGLIDKHLKP LDFRRKIADL ETLEEDELKD EGYIETYERY
660 670 680 690 700
VPKSWMKVPE DITRCFVSLL YTDANEGDNH IFHPLVFVQA HSFPKIDSFI
710 720 730 740 750
LNSTVGPRVK IVLETIEDSF KIDSHLLELL KKSTRYLLQF GLSTSLEQQI
760 770 780 790 800
PTPYWLAPLN LSCTDYRFLE NLIDVDTIQN FFKLPEPVQN VTDLQSDTVL
810 820 830 840 850
LVNPQSIYEQ YAFEGFVNSE FMIPAKKKDK APSALCKKLP LRLNYSLWGN
860 870 880 890 900
RAKSIPKSQQ VRSFYINDLY ILPVSRHLKN SALLIPSILY HIENLLVASS
910 920 930 940 950
FIEHFRLDCK IDTACQALTS AESQLNFDYD RLEFYGDCFL KLGASITVFL
960 970 980 990 1000
KFPDTQEYQL HFNRKKIISN CNLYKVAIDC ELPKYALSTP LEIRHWCPYG
1010 1020 1030 1040 1050
FQKSTSDKCR YAVLQKLSVK RIADMVEASI GACLLDSGLD SALKICKSLS
1060 1070 1080 1090 1100
VGLLDISNWD EWNNYFDLNT YADSLRNVQF PYSSYIEETI GYSFKNKKLL
1110 1120 1130 1140 1150
HLAFIHPSMM SQQGIYENYQ QLEFLGDAVL DYIIVQYLYK KYPNATSGEL
1160 1170 1180 1190 1200
TDYKSFYVCN KSLSYIGFVL NLHKYIQHES AAMCDAIFEY QELIEAFRET
1210 1220 1230 1240 1250
ASENPWFWFE IDSPKFISDT LEAMICAIFL DSGFSLQSLQ FVLPLFLNSL
1260 1270 1280 1290 1300
GDATHTKAKG DIEHKVYQLL KDQGCEDFGT KCVIEEVKSS HKTLLNTELH
1310 1320 1330 1340 1350
LTKYYGFSFF RHGNIVAYGK SRKVANAKYI MKQRLLKLLE DKSNLLLYSC
1360 1370
NCKFSKKKPS DEQIKGDGKV KSLT
Length:1,374
Mass (Da):158,040
Last modified:February 1, 1996 - v1
Checksum:i89AE9EF8DE7966C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAB41233.1.
PIRiT39130. S62524.
RefSeqiNP_588215.2. NM_001023205.2.

Genome annotation databases

EnsemblFungiiSPCC188.13c.1; SPCC188.13c.1:pep; SPCC188.13c.
GeneIDi2538930.
KEGGispo:SPCC188.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAB41233.1.
PIRiT39130. S62524.
RefSeqiNP_588215.2. NM_001023205.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L6MNMR-A1259-1358[»]
ProteinModelPortaliQ09884.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275506. 184 interactions.
MINTiMINT-4696006.

PTM databases

SwissPalmiQ09884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC188.13c.1; SPCC188.13c.1:pep; SPCC188.13c.
GeneIDi2538930.
KEGGispo:SPCC188.13c.

Organism-specific databases

EuPathDBiFungiDB:SPCC188.13c.
PomBaseiSPCC188.13c. dcr1.

Phylogenomic databases

InParanoidiQ09884.
KOiK11592.
OrthoDBiEOG092C0CUQ.
PhylomeDBiQ09884.

Miscellaneous databases

PROiQ09884.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 2 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCR1_SCHPO
AccessioniPrimary (citable) accession number: Q09884
Secondary accession number(s): Q9UUN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.