Q09879 (UBP5_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase 5
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5

Gene names

Name:	ubp5
ORF Names:	SPCC188.08c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	1108 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 MATH domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from mutant phenotype PubMed 20838651. Source: PomBase protein deubiquitination Inferred from direct assay PubMed 20838651. Source: PomBase ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 20838651. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase transport vesicle Inferred from direct assay PubMed 20838651. Source: PomBase
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay PubMed 20838651. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1108	1108	Probable ubiquitin carboxyl-terminal hydrolase 5		PRO_0000080606
Regions
	Domain	55 – 187	133	MATH
Sites
	Active site	222	1	Nucleophile By similarity
	Active site	464	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09879 [UniParc]. Last modified February 21, 2001. Version 3. Checksum: 5C5A57058E3839C7	FASTA	1,108	128,668
	10 20 30 40 50 60 MVTGETLVDS QKSLINNDTL LNEKLKEDFE ENVSIDVKIH EELRRALPDY EESGFQRFTW 70 80 90 100 110 120 HIKSWHELDR RAVSPQFAVG SRQFKITYFP QGTLQSAGFT SIFLEYIPSE EEKLSNKYGC 130 140 150 160 170 180 CCQFAFVISN PRKPSLSVAN SAHCRFSPEI VDWGFTQFAE LKKLLCRQAP DVPPIVEDGA 190 200 210 220 230 240 LLLTAYVRIL KDPTGVLWHS FNDYDSKIAT GYVGLKNQGA TCYMNSLLQS LYIIHAFRRI 250 260 270 280 290 300 VYQIPTDSPQ GKDSIAYALQ RCFYNLQFMN EPVSTTELTK SFGWDSLDSF MQHDVQEFNR 310 320 330 340 350 360 VLQDNLERSM RDTKVENALT NLFVGKMKSY IACVNVNFES ARSEDYWDIQ LNVKGMKNLE 370 380 390 400 410 420 DSFRSYIQVE TLEGDNCYFA DTYGFQEAKK GVIFESFPPI LHLQLKRFEY DFERDMMIKI 430 440 450 460 470 480 NDRYEFPLEF DAKAFLSPEA DQSQNCEYVL YGVLVHSGDL HNGHYYALLK TEKDGPWYKY 490 500 510 520 530 540 DDTRVTRATL REVLEENYGG DYIMHPPFRS PVKLKRFMSA YMLLYLRKDK LDELMNPVSA 550 560 570 580 590 600 DEIPEHLKEA LNPSIQLAEL RRKERLESHL YTKVQLITPE FYSEHHEFDI ADFGNAYKEE 610 620 630 640 650 660 TIPQFRIKKE AKFSEFIPIV AEKLGYPQEC MRFWYVVKRH NCTVRVESPV NELNSTMEEV 670 680 690 700 710 720 KNVWNSQGEI LRLYLEITPE NELSSSLTHQ NTGEWNAFIF VKYFDRKSQE ISGCGTLHVN 730 740 750 760 770 780 KSDEIRSICP LLCERANLPK NTPLNIYEEI KPGMVDFLRL EKTFTQSELS TGDIICFEPC 790 800 810 820 830 840 RPSALEDDIV NSGFDSALKL YDFLSNKVLV LFRPRFIDQD SIIEFEMLLD RRIKYDDLCI 850 860 870 880 890 900 ELGQKLGIGA DHIRLTTCNP LTYSAGMVVP NDSNITLYEI LYSSEEEMPS NVIFYETMDV 910 920 930 940 950 960 SLSDLDRKRL VRLRWLVDGL ANIELVEAYI NKSGDINDLF GAVCERFPDS DLRKKKVRVY 970 980 990 1000 1010 1020 EVFESRYHRD LSLRTLIRTI NPAATLVGEV VPLDQLQLYP EEKIVQVHHF HKDIARIHGI 1030 1040 1050 1060 1070 1080 PFSFVIKPQE KFIDTKLRLA ARTQYPESIF SVIKFCVVDF DNNRVVYLND EDITYDVVEK 1090 1100 LNGTLALDRA KKDSKKPNIL DRAIQMKN

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAB41228.1.
PIR	T41188.
RefSeq	NP_588211.1. NM_001023201.2.
3D structure databases
ProteinModelPortal	Q09879.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPCC188.08c-1.
Protein family/group databases
MEROPS	C19.A59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC188.08c.1; SPCC188.08c.1:pep; SPCC188.08c.
GeneID	2539385.
KEGG	spo:SPCC188.08c.
Organism-specific databases
PomBase	SPCC188.08c.
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000160240.
KO	K11838.
OMA	EDIFLFF.
OrthoDB	EOG4HDX2M.
Family and domain databases
InterPro	IPR002083. MATH. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR008974. TRAF-like. [Graphical view]
Pfam	PF00917. MATH. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00061. MATH. 1 hit. [Graphical view]
SUPFAM	SSF49599. Traf_like. 1 hit.
PROSITE	PS50144. MATH. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20800549.

Entry information

Entry name

UBP5_SCHPO

Accession

Primary (citable) accession number: Q09879
Secondary accession number(s): Q10433, Q9Y7P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 21, 2001
Last modified:	May 1, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program