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Protein

Probable sulfite reductase [NADPH] flavoprotein component

Gene

SPCC584.01c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate (By similarity).By similarity

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] subunit beta (sir1), Probable sulfite reductase [NADPH] flavoprotein component (SPCC584.01c)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi658 – 66912FADBy similarityAdd
BLAST
Nucleotide bindingi788 – 79811FAD (flavin part)By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Methionine biosynthesis, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable sulfite reductase [NADPH] flavoprotein component (EC:1.8.1.2)
Gene namesi
ORF Names:SPCC584.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC584.01c.
PomBaseiSPCC584.01c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • sulfite reductase complex (NADPH) Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10061006Probable sulfite reductase [NADPH] flavoprotein componentPRO_0000199948Add
BLAST

Proteomic databases

MaxQBiQ09878.

Interactioni

Protein-protein interaction databases

BioGridi275402. 38 interactions.
MINTiMINT-4695952.

Structurei

3D structure databases

ProteinModelPortaliQ09878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini622 – 852231FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000216012.
InParanoidiQ09878.
KOiK00380.
OMAiDKIFSYS.
OrthoDBiEOG092C1RRH.
PhylomeDBiQ09878.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
3.40.920.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR017938. Riboflavin_synthase-like_b-brl.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00371. FPNCR.
SUPFAMiSSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVATDSSDQV AKFDVSHKII EDIVYSLSNR IFSYAEHVPL NRYLTQWNNS
60 70 80 90 100
GRRNGFSNNV ELFNLEARSG ASAFLLGSYT AAISSPGVYS MMTPSSCLSL
110 120 130 140 150
LNPLLSNIIP FYGASKPLVV HVAALAYLEQ TYCADNVSVL DFSYANNFTV
160 170 180 190 200
FASQSNVEAA HLALASTLAA KAAPVIHVYE PDAIVTTTDP SLPLLDSNAV
210 220 230 240 250
VECFNSYQSE ADPVSNASKA LKHVNDYFNT SYAPAEYYGS QTASKVIVTF
260 270 280 290 300
GKSETVAARA LLAANPDVGV LSIRIFPFVA ENIFNVLPTT CKSLVVLSQV
310 320 330 340 350
RSTAVGTSSI YYSFLLATLL STKPSALAIS EHRYSLVESV TLSSLFDALH
360 370 380 390 400
ETLQLKAATP KAVHVDKSIN VWESDVGDSL VLSLVSAYRT DKSRSVAFRP
410 420 430 440 450
LFDNLTLAGV RFTVAQVSTA NAVLTDVVKD VDADITILTT DRLPLHYRVL
460 470 480 490 500
AKAAEHSICL LQSSIAPDEA TKKLPYEFIA DALEKGVKLV LIDPKKFAID
510 520 530 540 550
ASNLPLLVSF IQLVKPGLGV DEALAVLAKQ NNLTDTNLKD AVDSLKQSLS
560 570 580 590 600
FINLDASALK DREPSEKELP STAKETSFAP NAVKTLDEDI TPQSSNWQTV
610 620 630 640 650
AKQIIFPEAY KKKDALRPDV SEKVFTVHVR ANKRLTPAEY NRNIFHIEFD
660 670 680 690 700
LGDSGLTYDI GEALGVYGVN NKTHVHDFIE EYGLDANELI HVPSIQHPGH
710 720 730 740 750
WETRTVFQAL CQNIDIFGKP TKKFHEQLLE FETDEKERAD LQILISPAGA
760 770 780 790 800
PDFKRRAEVD MLTYADVLKE FKHAKLTAAQ IAQIVPVIKR REYSISSSQK
810 820 830 840 850
KHNDSVHLLV VVVGWKDGMG RDRYGQCSHY LSNLKVGEPL CVAVKTSVMK
860 870 880 890 900
LPTSPLKPIV MAGLGTGLAP FRAFLQFKEW QRMQGIESGD ILLYLGSRTQ
910 920 930 940 950
REEYLYGEDW EAYHSANLLT HIGQAFSRDQ PYKIYIQDVM RSTKDMLKKA
960 970 980 990 1000
LMDEGGSFYL CGPTWPLPEI TSVLEEVIQS SYDEPVDARK IIEQWKEERR

FVIEVY
Length:1,006
Mass (Da):111,353
Last modified:May 30, 2000 - v2
Checksum:i2EA2086D69561D33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21818.2.
PIRiT41439.
RefSeqiNP_588222.1. NM_001023212.2.

Genome annotation databases

EnsemblFungiiSPCC584.01c.1; SPCC584.01c.1:pep; SPCC584.01c.
GeneIDi2538821.
KEGGispo:SPCC584.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21818.2.
PIRiT41439.
RefSeqiNP_588222.1. NM_001023212.2.

3D structure databases

ProteinModelPortaliQ09878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275402. 38 interactions.
MINTiMINT-4695952.

Proteomic databases

MaxQBiQ09878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC584.01c.1; SPCC584.01c.1:pep; SPCC584.01c.
GeneIDi2538821.
KEGGispo:SPCC584.01c.

Organism-specific databases

EuPathDBiFungiDB:SPCC584.01c.
PomBaseiSPCC584.01c.

Phylogenomic databases

HOGENOMiHOG000216012.
InParanoidiQ09878.
KOiK00380.
OMAiDKIFSYS.
OrthoDBiEOG092C1RRH.
PhylomeDBiQ09878.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.

Miscellaneous databases

PROiQ09878.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
3.40.920.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR017938. Riboflavin_synthase-like_b-brl.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00371. FPNCR.
SUPFAMiSSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMET10_SCHPO
AccessioniPrimary (citable) accession number: Q09878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.