Reviewed, UniProtKB/Swiss-Prot Q09860 (SYCC_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable cysteinyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

ORF Names:

SPAC29E6.06c, SPAC30.10c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	Probable cysteinyl-tRNA synthetase, cytoplasmic		PRO_0000159552
Regions
	Motif	48 – 58	11	"HIGH" region
	Motif	408 – 412	5	"KMSKS" region
Sites
	Metal binding	46	1	Zinc By similarity
	Metal binding	350	1	Zinc By similarity
	Metal binding	375	1	Zinc By similarity
	Metal binding	379	1	Zinc By similarity
	Binding site	411	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09860-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 985E0301CC105358	FASTA	754	85,555
	10 20 30 40 50 60 MSTRNPAQSH WGVPKGQRTE LYVYNTLTHS KVPFVSNGSN LTWYCCGPTV YDASHMGHAR 70 80 90 100 110 120 NYVTTDILRR ILQSYFGYNI TFVQNVTDID DKIILRARQQ YLFEEYKKQQ GTNKSHSEAR 130 140 150 160 170 180 EKVTEAWFAY AKKNLPEPPS SMSEWPQWLA SHDIPTLAIN NPKLPMHVDA LKSALDALQV 190 200 210 220 230 240 SEASEIISLD GFWPKVQDVL VPLLDAELGS TVTDPAIFRK LAAYWEDDFN KDMANLNVLP 250 260 270 280 290 300 PTAVTRVSEY VPEIVDFVQR IIDRGYAYPV TDGSVYFDTE AFEKGGHFYA KLEPWNKGNR 310 320 330 340 350 360 ELIAEGEGSL AALTGKKRPG DFALWKASKP GEPSWDSPWS KGRPGWHIEC SVMASALLGS 370 380 390 400 410 420 NIDIHSGGID LAFPHHDNEL AQSEAYFDCP QWVNYFFHAG HLHIEGQKMS KSLKNFITIK 430 440 450 460 470 480 EILKKFTPRQ LRLAFLLQQW NTQLDFKETL LAYVLNIEQA LENFFRTVRA LMNETEGVSA 490 500 510 520 530 540 NGGHVPEKFD KLEIELLEKF QQTQQNTHLA LCDSFNTPLV MQHIDNLVTQ ANIYIREVGQ 550 560 570 580 590 600 QPCSRLLGQI ASWITSMLQI FGLDENGHPN AVGWSSSKGS SSENTDAMPY IRAVSSFRDR 610 620 630 640 650 660 VRELCIAKAS SQEILKACDI FRDYDMAALG VSFNDRPQGS ALVKLVDAEE LIAAREQKLE 670 680 690 700 710 720 EERAKQAKKA QAKAEQEKKQ VERVMKGKTS PSEMFKMFKE YLSFDEAGLP TKMRGEDGSE 730 740 750 IDVPKSRKKK LQKEYAQQEK LHKEYLSYIE SNKS

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z66525 Genomic DNA. Translation: CAA91428.1. CU329670 Genomic DNA. Translation: CAB66469.1.
PIR	S62512. T38507.
RefSeq	NP_594564.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	2542721.
KEGG	spo:SPAC29E6.06c.
NMPDR	fig|4896.1.peg.4534.
Organism-specific databases
GeneDB_Spombe	SPAC29E6.06c.
Phylogenomic databases
OMA	Q09860. IERIARW.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002660-MON.
BRENDA	6.1.1.16. 653.
Gene expression databases
ArrayExpress	Q09860.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_SCHPO

Accession

Primary (citable) accession number: Q09860

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)