Reviewed, UniProtKB/Swiss-Prot Q09850 (ENG2_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative endo-1,3(4)-beta-glucanase 2
      Short name=Endo-1,4-beta-glucanase 2
      Short name=Endo-1,3-beta-glucanase 2
    EC=3.2.1.6
Alternative name(s):
    Laminarinase-2

Gene names

Name:	eng2
ORF Names:	SPAC23D3.10c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 81 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred by curator. Source: GeneDB_SPombe
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endo-1,3(4)-beta-glucanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Putative endo-1,3(4)-beta-glucanase 2		PRO_0000215818
Regions
	Transmembrane	137 – 157	21	Potential
	Transmembrane	307 – 327	21	Potential
	Transmembrane	460 – 480	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q09850-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7AA4A9BED26C37B6	FASTA	706	77,948
	10 20 30 40 50 60 MDVLVPIYTG PINPVFPSRA HPSPPLGLTS NLFPIQTNKF YGNLYIGTRH NPSWSHPYSV 70 80 90 100 110 120 TWLNGSSYYG LAISHIDDSQ RVFGPDPESV PCQYYFNPAG LYSIIISARE FASGNLLSLD 130 140 150 160 170 180 QSRHFSIQAT LSATTSGSGT IILPIVAGMG FVSGYYTNLT PVFNSSILFS SITKINFSRG 190 200 210 220 230 240 YKYRIQLTDG KIWFLYAFPT SSSSTFNLTL ASNSQLTTST KFTGLIQICK VPNESVNNSY 250 260 270 280 290 300 PDTIYDASAG VYTTSISLSA QVSGTTGEYW FRFATAGYTN LNPLMFALPH HMQSFGSDTQ 310 320 330 340 350 360 AYKTGLGLAS TTMGIMFAYA TKTWHLIEKN LPTQVGFLPI PWNGGSNTYS PTALAAIRAA 370 380 390 400 410 420 CATDINFDVV NASNLDSMYT SGKIVAMYAQ VCLVASRILG DSTLTNTGLT KLKQAMARFT 430 440 450 460 470 480 TNTQMYPLVY DTTYKGIIST AGYSSPLADY GNTYYNDHHF HWGYHIYACA VIGLLDPSWL 490 500 510 520 530 540 VNDNIRYVNA LLRDSANPSE SDTYFAMFRN FDWFVGHSWA TGIFESGDGK DEESTSEDFN 550 560 570 580 590 600 FLYATKLWGM VRNDTVLINR ANLMLAVLKN SLNTYIYMTP TTSVQPSQIL GNYVTGITFM 610 620 630 640 650 660 NKVDYATYFS AEEYCKQGIH MIPTTPISGY LRSPSYVQQD WNAKIAPIIN TFSNNWTGIL 670 680 690 700 WSNYAIYDPK TAYNTFAAST FTADKIDNGA SKTWYLAMAA GMGGSP

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA91245.1.
PIR	S62501.
RefSeq	NP_594547.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH81. Glycoside Hydrolase Family 81.
Genome annotation databases
GeneID	2541480.
KEGG	spo:SPAC23D3.10c.
NMPDR	fig|4896.1.peg.4517.
Organism-specific databases
GeneDB_Spombe	SPAC23D3.10c.
Phylogenomic databases
OMA	Q09850. RTWSLAY.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002644-MON.
BRENDA	3.2.1.6. 653.
Gene expression databases
ArrayExpress	Q09850.
Family and domain databases
InterPro	IPR005200. Glyco_hydro_81. [Graphical view]
Pfam	PF03639. Glyco_hydro_81. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENG2_SCHPO

Accession

Primary (citable) accession number: Q09850

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)