Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-amylase 2

Gene

aah2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109SubstrateBy similarity1
Metal bindingi147Calcium 1By similarity1
Metal bindingi202Calcium 1By similarity1
Binding sitei231SubstrateBy similarity1
Active sitei233NucleophileBy similarity1
Metal bindingi233Calcium 2By similarity1
Metal bindingi237Calcium 1; via carbonyl oxygenBy similarity1
Active sitei257Proton donorBy similarity1
Metal bindingi257Calcium 2By similarity1
Binding sitei261Substrate; via amide nitrogenBy similarity1
Binding sitei325SubstrateBy similarity1
Sitei325Transition state stabilizerBy similarity1
Binding sitei372SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 2 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:aah2
ORF Names:SPAC23D3.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23D3.14c.
PomBaseiSPAC23D3.14c. aah2.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: PomBase
  • endoplasmic reticulum Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000135525 – 551Alpha-amylase 2Add BLAST527
PropeptideiPRO_0000255452552 – 581Removed in mature formSequence analysisAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 64By similarity
Disulfide bondi176 ↔ 191By similarity
Disulfide bondi267 ↔ 311By similarity
Glycosylationi291N-linked (GlcNAc...)Sequence analysis1
Glycosylationi332N-linked (GlcNAc...)Sequence analysis1
Lipidationi551GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiQ09840.

Interactioni

Protein-protein interaction databases

MINTiMINT-4695620.

Structurei

3D structure databases

ProteinModelPortaliQ09840.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 237Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiQ09840.
KOiK01176.
OrthoDBiEOG092C1HLH.
PhylomeDBiQ09840.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYRRNICLR IGWMLLFAFI PAYAGHSAEE WKRRSIYQII TDRFSLEEGA
60 70 80 90 100
TERIPCDPVR FMYCGGTWNG IRNHLDYIQG MGFDAIWISP IFENVEGNDI
110 120 130 140 150
DGSSYHGYWT TNLYELNHHF GTKEEFMELI QELHKRDIWI LLDVAINSMA
160 170 180 190 200
INGPLEQMSF EKVIPFNDAS FFHPHCWVDY ESNDIESVQN CWLGDENLLL
210 220 230 240 250
ADVDTENEVV LSVLEKWIKN VVQEYDIDGI RFDAIKHAPI EFWLRMSKAA
260 270 280 290 300
DIFTIGEYFT GSPAEACDYQ NSGLDSFLNF PLYWPITWAF NNTGLQCEAL
310 320 330 340 350
AIAINQINEE CNDINVLGTF IGNHDLPRIS HNNTDQARIM NAITFVMMWD
360 370 380 390 400
GIPIIYYGTE QNFNSYHDPF NREALWLSNF DMENVYYKLI GILNRFRKSV
410 420 430 440 450
QRQEENYVNT RSTILSVKIH HIVVQKLNVI TVLNNYGIHN EERLSIVFKP
460 470 480 490 500
LGASPKDTFF DIINNQKYVV NTDGTLKVVI TNGFPIVLYP TSKIETSLPQ
510 520 530 540 550
FTATLLPEIT FVPSITVTTH YVLPTLLAPL GYDIREHPGG QQFWNTLTAK
560 570 580
SEAKTIRSFT KLKLFILLIA VPFALPMIIL I
Length:581
Mass (Da):67,005
Last modified:February 1, 1996 - v1
Checksum:iFE9DE99D323E1890
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91249.1.
PIRiT38289. S62505.
RefSeqiNP_594551.1. NM_001019980.1.

Genome annotation databases

EnsemblFungiiSPAC23D3.14c.1; SPAC23D3.14c.1:pep; SPAC23D3.14c.
GeneIDi2541501.
KEGGispo:SPAC23D3.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91249.1.
PIRiT38289. S62505.
RefSeqiNP_594551.1. NM_001019980.1.

3D structure databases

ProteinModelPortaliQ09840.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4695620.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiQ09840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23D3.14c.1; SPAC23D3.14c.1:pep; SPAC23D3.14c.
GeneIDi2541501.
KEGGispo:SPAC23D3.14c.

Organism-specific databases

EuPathDBiFungiDB:SPAC23D3.14c.
PomBaseiSPAC23D3.14c. aah2.

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiQ09840.
KOiK01176.
OrthoDBiEOG092C1HLH.
PhylomeDBiQ09840.

Miscellaneous databases

PROiQ09840.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY2_SCHPO
AccessioniPrimary (citable) accession number: Q09840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 5, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.