SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q09840

- AMY2_SCHPO

UniProt

Q09840 - AMY2_SCHPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Alpha-amylase 2

Gene
aah2, SPAC23D3.14c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091Substrate By similarity
Metal bindingi147 – 1471Calcium 1 By similarity
Metal bindingi202 – 2021Calcium 1 By similarity
Binding sitei231 – 2311Substrate By similarity
Active sitei233 – 2331Nucleophile By similarity
Metal bindingi233 – 2331Calcium 2 By similarity
Metal bindingi237 – 2371Calcium 1; via carbonyl oxygen By similarity
Active sitei257 – 2571Proton donor By similarity
Metal bindingi257 – 2571Calcium 2 By similarity
Binding sitei261 – 2611Substrate; via amide nitrogen By similarity
Binding sitei324 – 3241Substrate By similarity
Sitei325 – 3251Transition state stabilizer By similarity
Binding sitei372 – 3721Substrate By similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. cellular polysaccharide catabolic process Source: PomBase
  2. extracellular polysaccharide metabolic process Source: PomBase
  3. fungal-type cell wall biogenesis Source: PomBase
  4. regulation of cell shape Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 2 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:aah2
ORF Names:SPAC23D3.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC23D3.14c.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor Reviewed prediction 2 Publications

GO - Cellular componenti

  1. anchored component of external side of plasma membrane Source: PomBase
  2. endoplasmic reticulum Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 551527Alpha-amylase 2PRO_0000001355Add
BLAST
Propeptidei552 – 58130Removed in mature form Reviewed predictionPRO_0000255452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 64 By similarity
Disulfide bondi176 ↔ 191 By similarity
Disulfide bondi267 ↔ 311 By similarity
Glycosylationi291 – 2911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi332 – 3321N-linked (GlcNAc...) Reviewed prediction
Lipidationi551 – 5511GPI-anchor amidated serine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Protein-protein interaction databases

MINTiMINT-4695620.
STRINGi4896.SPAC23D3.14c-1.

Structurei

3D structure databases

ProteinModelPortaliQ09840.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2372Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000165530.
KOiK01176.
OMAiCWIDYSN.
OrthoDBiEOG7RBZJ4.
PhylomeDBiQ09840.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013777. A-amylase_fun.
IPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
PIRSFiPIRSF001024. Alph-amyl_fung. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09840-1 [UniParc]FASTAAdd to Basket

« Hide

MNYRRNICLR IGWMLLFAFI PAYAGHSAEE WKRRSIYQII TDRFSLEEGA    50
TERIPCDPVR FMYCGGTWNG IRNHLDYIQG MGFDAIWISP IFENVEGNDI 100
DGSSYHGYWT TNLYELNHHF GTKEEFMELI QELHKRDIWI LLDVAINSMA 150
INGPLEQMSF EKVIPFNDAS FFHPHCWVDY ESNDIESVQN CWLGDENLLL 200
ADVDTENEVV LSVLEKWIKN VVQEYDIDGI RFDAIKHAPI EFWLRMSKAA 250
DIFTIGEYFT GSPAEACDYQ NSGLDSFLNF PLYWPITWAF NNTGLQCEAL 300
AIAINQINEE CNDINVLGTF IGNHDLPRIS HNNTDQARIM NAITFVMMWD 350
GIPIIYYGTE QNFNSYHDPF NREALWLSNF DMENVYYKLI GILNRFRKSV 400
QRQEENYVNT RSTILSVKIH HIVVQKLNVI TVLNNYGIHN EERLSIVFKP 450
LGASPKDTFF DIINNQKYVV NTDGTLKVVI TNGFPIVLYP TSKIETSLPQ 500
FTATLLPEIT FVPSITVTTH YVLPTLLAPL GYDIREHPGG QQFWNTLTAK 550
SEAKTIRSFT KLKLFILLIA VPFALPMIIL I 581
Length:581
Mass (Da):67,005
Last modified:February 1, 1996 - v1
Checksum:iFE9DE99D323E1890
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA91249.1.
PIRiT38289. S62505.
RefSeqiNP_594551.1. NM_001019980.1.

Genome annotation databases

EnsemblFungiiSPAC23D3.14c.1; SPAC23D3.14c.1:pep; SPAC23D3.14c.
GeneIDi2541501.
KEGGispo:SPAC23D3.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA91249.1 .
PIRi T38289. S62505.
RefSeqi NP_594551.1. NM_001019980.1.

3D structure databases

ProteinModelPortali Q09840.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4695620.
STRINGi 4896.SPAC23D3.14c-1.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC23D3.14c.1 ; SPAC23D3.14c.1:pep ; SPAC23D3.14c .
GeneIDi 2541501.
KEGGi spo:SPAC23D3.14c.

Organism-specific databases

PomBasei SPAC23D3.14c.

Phylogenomic databases

eggNOGi COG0366.
HOGENOMi HOG000165530.
KOi K01176.
OMAi CWIDYSN.
OrthoDBi EOG7RBZJ4.
PhylomeDBi Q09840.

Miscellaneous databases

NextBioi 20802600.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR013777. A-amylase_fun.
IPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view ]
PIRSFi PIRSF001024. Alph-amyl_fung. 1 hit.
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Genome-wide identification of fungal GPI proteins."
    De Groot P.W., Hellingwerf K.J., Klis F.M.
    Yeast 20:781-796(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PREDICTION OF GPI-ANCHOR.
  3. "An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe."
    Morita T., Tanaka N., Hosomi A., Giga-Hama Y., Takegawa K.
    Biosci. Biotechnol. Biochem. 70:1454-1463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAMY2_SCHPO
AccessioniPrimary (citable) accession number: Q09840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi