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Protein

tRNA (uracil(54)-C(5))-methyltransferase

Gene

SPAC4G8.07c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + uracil(54) in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil(54) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi182Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi188Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi191Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi276Iron-sulfur (4Fe-4S)By similarity1
Binding sitei347S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei383S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei404S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei451S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei478NucleophilePROSITE-ProRule annotation1
Active sitei517Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil(54)-C(5))-methyltransferase (EC:2.1.1.35)
Gene namesi
ORF Names:SPAC4G8.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G8.07c.
PomBaseiSPAC4G8.07c.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001620601 – 527tRNA (uracil(54)-C(5))-methyltransferaseAdd BLAST527

Proteomic databases

MaxQBiQ09833.
PRIDEiQ09833.

Interactioni

Protein-protein interaction databases

MINTiMINT-4695563.

Structurei

3D structure databases

ProteinModelPortaliQ09833.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 167TRAMPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation
Contains 1 TRAM domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000029869.
InParanoidiQ09833.
KOiK15331.
OMAiVLDIEEC.
OrthoDBiEOG092C156C.
PhylomeDBiQ09833.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR012340. NA-bd_OB-fold.
IPR029063. SAM-dependent_MTases.
IPR002792. TRAM_dom.
IPR025795. tRNA_(uracil-5-)_MeTrfase.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF01938. TRAM. 1 hit.
PF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS50926. TRAM. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPYCLKYLT NGLRSKQMIN FLCIQNILSA RTLKICRFRF LPTPSHPTHK
60 70 80 90 100
MAQETRAISS ISEAANKRAR KLSKRRAKKP VEEGSSGHVL LLEIENLIEK
110 120 130 140 150
PVLANVPFER FQEIEVKISY LSSSGDGIGL VCNDQYAVVV PFTLPGDIVK
160 170 180 190 200
AKLHFLADTY ALADFLEVIS PSEDRDDTLI KCPYFAKCGG CQYQMLSYDK
210 220 230 240 250
QLLQKKRVVE KAFQYYSKLD SSLLPAIKDT VGSPLQYNYR TKITPHFDVP
260 270 280 290 300
KGGTKGPLII GFQEKGRRRV MDIEECPIAT KTINEEYPKI IEDVQSRANT
310 320 330 340 350
FKRGATILMR DSATEDGKHC VITDHKMIVR EQFGDLSFTF PAGAFFQNNN
360 370 380 390 400
SILEKFTSYV REQLLNPFGR KEHQRPKYFV DAYCGSGLFS VACSKGFLSV
410 420 430 440 450
IGVEISADSV RYAEENAKRN NVSNATFIVG QAEKIFSSIE TPPNETAMVI
460 470 480 490 500
DPPRKGCDQS FLNQLLEYSP YRIVYISCNV HTQARDVGFL LSQEKGRSYK
510 520
IDEIRGFDLF PQSHHVESIL TLTKVVS
Length:527
Mass (Da):59,614
Last modified:February 1, 1996 - v1
Checksum:iACCB80D8C6FFB77F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91208.1.
PIRiT38852. S62484.
RefSeqiNP_593067.1. NM_001018465.2.

Genome annotation databases

EnsemblFungiiSPAC4G8.07c.1; SPAC4G8.07c.1:pep; SPAC4G8.07c.
GeneIDi2543535.
KEGGispo:SPAC4G8.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91208.1.
PIRiT38852. S62484.
RefSeqiNP_593067.1. NM_001018465.2.

3D structure databases

ProteinModelPortaliQ09833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4695563.

Proteomic databases

MaxQBiQ09833.
PRIDEiQ09833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4G8.07c.1; SPAC4G8.07c.1:pep; SPAC4G8.07c.
GeneIDi2543535.
KEGGispo:SPAC4G8.07c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4G8.07c.
PomBaseiSPAC4G8.07c.

Phylogenomic databases

HOGENOMiHOG000029869.
InParanoidiQ09833.
KOiK15331.
OMAiVLDIEEC.
OrthoDBiEOG092C156C.
PhylomeDBiQ09833.

Miscellaneous databases

PROiQ09833.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR012340. NA-bd_OB-fold.
IPR029063. SAM-dependent_MTases.
IPR002792. TRAM_dom.
IPR025795. tRNA_(uracil-5-)_MeTrfase.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF01938. TRAM. 1 hit.
PF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS50926. TRAM. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYAD7_SCHPO
AccessioniPrimary (citable) accession number: Q09833
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 5, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.