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Protein

tRNA (uracil(54)-C(5))-methyltransferase

Gene

SPAC4G8.07c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + uracil(54) in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil(54) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi182 – 1821Iron-sulfur (4Fe-4S)By similarity
Metal bindingi188 – 1881Iron-sulfur (4Fe-4S)By similarity
Metal bindingi191 – 1911Iron-sulfur (4Fe-4S)By similarity
Metal bindingi276 – 2761Iron-sulfur (4Fe-4S)By similarity
Binding sitei347 – 3471S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei383 – 3831S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei404 – 4041S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei451 – 4511S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei478 – 4781NucleophilePROSITE-ProRule annotation
Active sitei517 – 5171Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil(54)-C(5))-methyltransferase (EC:2.1.1.35)
Gene namesi
ORF Names:SPAC4G8.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G8.07c.
PomBaseiSPAC4G8.07c.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527tRNA (uracil(54)-C(5))-methyltransferasePRO_0000162060Add
BLAST

Proteomic databases

MaxQBiQ09833.
PRIDEiQ09833.

Interactioni

Protein-protein interaction databases

MINTiMINT-4695563.

Structurei

3D structure databases

ProteinModelPortaliQ09833.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 16761TRAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation
Contains 1 TRAM domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000029869.
InParanoidiQ09833.
KOiK15331.
OMAiADFFPQT.
OrthoDBiEOG7RV9QV.
PhylomeDBiQ09833.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR012340. NA-bd_OB-fold.
IPR029063. SAM-dependent_MTases.
IPR002792. TRAM_dom.
IPR025795. tRNA_(uracil-5-)_MeTrfase.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF01938. TRAM. 1 hit.
PF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS50926. TRAM. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPYCLKYLT NGLRSKQMIN FLCIQNILSA RTLKICRFRF LPTPSHPTHK
60 70 80 90 100
MAQETRAISS ISEAANKRAR KLSKRRAKKP VEEGSSGHVL LLEIENLIEK
110 120 130 140 150
PVLANVPFER FQEIEVKISY LSSSGDGIGL VCNDQYAVVV PFTLPGDIVK
160 170 180 190 200
AKLHFLADTY ALADFLEVIS PSEDRDDTLI KCPYFAKCGG CQYQMLSYDK
210 220 230 240 250
QLLQKKRVVE KAFQYYSKLD SSLLPAIKDT VGSPLQYNYR TKITPHFDVP
260 270 280 290 300
KGGTKGPLII GFQEKGRRRV MDIEECPIAT KTINEEYPKI IEDVQSRANT
310 320 330 340 350
FKRGATILMR DSATEDGKHC VITDHKMIVR EQFGDLSFTF PAGAFFQNNN
360 370 380 390 400
SILEKFTSYV REQLLNPFGR KEHQRPKYFV DAYCGSGLFS VACSKGFLSV
410 420 430 440 450
IGVEISADSV RYAEENAKRN NVSNATFIVG QAEKIFSSIE TPPNETAMVI
460 470 480 490 500
DPPRKGCDQS FLNQLLEYSP YRIVYISCNV HTQARDVGFL LSQEKGRSYK
510 520
IDEIRGFDLF PQSHHVESIL TLTKVVS
Length:527
Mass (Da):59,614
Last modified:February 1, 1996 - v1
Checksum:iACCB80D8C6FFB77F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91208.1.
PIRiT38852. S62484.
RefSeqiNP_593067.1. NM_001018465.2.

Genome annotation databases

EnsemblFungiiSPAC4G8.07c.1; SPAC4G8.07c.1:pep; SPAC4G8.07c.
GeneIDi2543535.
KEGGispo:SPAC4G8.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91208.1.
PIRiT38852. S62484.
RefSeqiNP_593067.1. NM_001018465.2.

3D structure databases

ProteinModelPortaliQ09833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4695563.

Proteomic databases

MaxQBiQ09833.
PRIDEiQ09833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4G8.07c.1; SPAC4G8.07c.1:pep; SPAC4G8.07c.
GeneIDi2543535.
KEGGispo:SPAC4G8.07c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4G8.07c.
PomBaseiSPAC4G8.07c.

Phylogenomic databases

HOGENOMiHOG000029869.
InParanoidiQ09833.
KOiK15331.
OMAiADFFPQT.
OrthoDBiEOG7RV9QV.
PhylomeDBiQ09833.

Miscellaneous databases

PROiQ09833.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR012340. NA-bd_OB-fold.
IPR029063. SAM-dependent_MTases.
IPR002792. TRAM_dom.
IPR025795. tRNA_(uracil-5-)_MeTrfase.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF01938. TRAM. 1 hit.
PF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS50926. TRAM. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiYAD7_SCHPO
AccessioniPrimary (citable) accession number: Q09833
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.