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Protein

tRNA wybutosine-synthesizing protein 2

Gene

trm12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 4-demethylwyosine(37) in tRNA(Phe) = S-methyl-5'-thioadenosine + 7-((3S)-3-amino-3-carboxypropyl)-4-demethylwyosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei228S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei235S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 2 (EC:2.5.1.114)
Short name:
tRNA-yW-synthesizing protein 2
Alternative name(s):
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
Gene namesi
Name:trm12
Synonyms:tyw2
ORF Names:SPAC4G8.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G8.06c.
PomBaseiSPAC4G8.06c. trm12.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001164161 – 418tRNA wybutosine-synthesizing protein 2Add BLAST418

Proteomic databases

MaxQBiQ09832.
PRIDEiQ09832.

Interactioni

Protein-protein interaction databases

BioGridi279968. 21 interactors.
MINTiMINT-4695552.

Structurei

3D structure databases

ProteinModelPortaliQ09832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni275 – 276S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2
Regioni302 – 303S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ09832.
KOiK07055.
OMAiHIAINEP.
OrthoDBiEOG092C29GN.
PhylomeDBiQ09832.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR026274. tRNA_wybutosine_synth_prot_2.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
PIRSFiPIRSF038972. Trm12. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISFLVQKSE AKYWKDVVQN KNKFLKNIGI ISGPKLVDTY SNQFEADFLK
60 70 80 90 100
KCVLIPTSYN KEDLPKDILD CPRIYLIEEE VKSVQTDEKL QIKVLRYVSQ
110 120 130 140 150
ILHTTVSEEQ YPVPSRFVIY PPLALLSLNS FGTKEWHLFF QSYANVDVKA
160 170 180 190 200
GLFDIFAREW NVSHIAINEP IPVGDVMRRP LSLKPLYGDF GVLIDGNPSE
210 220 230 240 250
QDFQKAFWVS CRQNGIYQTW APLYTMFSRG NSIEKARVLN FSYIKDEIIA
260 270 280 290 300
DLYAGIGYFT FSYVKAGAST VFCWEINPWS VEALRRAALK NGWSIYVVRN
310 320 330 340 350
GENYEFKVGT HRIVVFLESN VYAAERFSKM NISARHINLG MLPSSEKSWS
360 370 380 390 400
TATSILKRES HSFIHVHENV KDEDIETYSS EVNSCFSKML AKNTVCVTNC
410
VKSFSPRVSH IVYDIETV
Length:418
Mass (Da):47,979
Last modified:February 1, 1996 - v1
Checksum:i82357027871B0B2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91207.1.
PIRiT38851. S62483.
RefSeqiNP_593066.1. NM_001018464.2.

Genome annotation databases

EnsemblFungiiSPAC4G8.06c.1; SPAC4G8.06c.1:pep; SPAC4G8.06c.
GeneIDi2543551.
KEGGispo:SPAC4G8.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91207.1.
PIRiT38851. S62483.
RefSeqiNP_593066.1. NM_001018464.2.

3D structure databases

ProteinModelPortaliQ09832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279968. 21 interactors.
MINTiMINT-4695552.

Proteomic databases

MaxQBiQ09832.
PRIDEiQ09832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4G8.06c.1; SPAC4G8.06c.1:pep; SPAC4G8.06c.
GeneIDi2543551.
KEGGispo:SPAC4G8.06c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4G8.06c.
PomBaseiSPAC4G8.06c. trm12.

Phylogenomic databases

InParanoidiQ09832.
KOiK07055.
OMAiHIAINEP.
OrthoDBiEOG092C29GN.
PhylomeDBiQ09832.

Enzyme and pathway databases

UniPathwayiUPA00375.

Miscellaneous databases

PROiQ09832.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR026274. tRNA_wybutosine_synth_prot_2.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
PIRSFiPIRSF038972. Trm12. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYW2_SCHPO
AccessioniPrimary (citable) accession number: Q09832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.