Reviewed, UniProtKB/Swiss-Prot Q09828 (SYLM_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative leucyl-tRNA synthetase, mitochondrial
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

ORF Names:

SPAC4G8.09

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW leucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 21	21	Mitochondrion By similarity
	Chain	22 – 874	853	Putative leucyl-tRNA synthetase, mitochondrial		PRO_0000035811
Regions
	Motif	67 – 77	11	"HIGH" region
	Motif	628 – 632	5	"KMSKS" region
Sites
	Binding site	631	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09828-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 0F9550E7E720AFC6	FASTA	874	100,886
	10 20 30 40 50 60 MLKSVGTNGR KVPKIASLCN FLKFNKNIHS NPDFLAIAEN WKSYWKSHYP FVKNDKGKKK 70 80 90 100 110 120 YILSMFPYPS GLLHIGHVRV YTISDILSRY YRMKGYKVIH PMGWDAFGLP AENAAIENGI 130 140 150 160 170 180 SASSWTYENI KKMKEQTYHM NIYFDWDREI STCNPDYYKW SQYLFLQMFH KGLAYQAEAT 190 200 210 220 230 240 VNWDPVDCTV LANEQVDSHG RSWRSGAIVE KKNLRQWFLK ISDYSDQLLD DLETLPKWPD 250 260 270 280 290 300 KVKKMQRNWI GRTTGFEISF PLLNDKETLT VFTTKPETII DVSFIALSKN HKLVLLESQK 310 320 330 340 350 360 DPSLAEHLRN ESLLDKGYQL PCFAKNPVTG KALPIFYAPY VLDCYGTGAV MGAPIHDRRD 370 380 390 400 410 420 FEFAKRNNII FSKESCIGSY FTNEGKELLN HHDSSNLMDI KQKMLQQKIV SYLEEKKLAK 430 440 450 460 470 480 RVKNYRLKDW LISRQRFWGT PIPMVHCETC GAVPVPESEL PVKLPDLDKI YEKGTSPLSN 490 500 510 520 530 540 LETWMKTTCP KCHGPATRET DTMDTFVDSS WYYFRFLDSK NSELPVGLAS ASSLMPVDIY 550 560 570 580 590 600 IGGVEHSILH LLYSRFFSKF MKDIGLWNGD KYLNEPFTQL ITQGMVHGLT YTTMSDERIL 610 620 630 640 650 660 NPKEVQKIGD EYFLISNPKE KVQLSYQKMS KSKHNGVDPI RTIQKYGSDI IRAYIIFSAP 670 680 690 700 710 720 VEGVLLWNEN AIMGTKRWLT KIWNCVHQLL EREKKMSDAM RQTKLTIVDD HNSRKLESQY 730 740 750 760 770 780 NEFISQCSAH YENVFSLNLV ISDAMKLTNN IADALKNNKV NIGTIKASLE VLVKCIAPII 790 800 810 820 830 840 PCFSSECWLL LGHNSSVYSN WPISKNKKEN MEEPVTIPVQ INGKVRFKIE MPKLNDENEI 850 860 870 LNFVLETNDG KRWLSNKKVL KSFVKQKIIS LVTD

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA91210.1.
PIR	S62486. T38854.
RefSeq	NP_593069.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2543476.
KEGG	spo:SPAC4G8.09.
NMPDR	fig|4896.1.peg.3039.
Organism-specific databases
GeneDB_Spombe	SPAC4G8.09.
Phylogenomic databases
OMA	Q09828. MMFASPP.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000784-MON.
BRENDA	6.1.1.4. 653.
Gene expression databases
ArrayExpress	Q09828.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 2 hits. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYLM_SCHPO

Accession

Primary (citable) accession number: Q09828

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)