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Protein

ATP-dependent helicase upf1

Gene

upf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Required for rapid turnover of mRNAs containing a premature translational termination codon.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei397 – 3971ATPBy similarity
Binding sitei593 – 5931ATPBy similarity
Binding sitei630 – 6301ATPBy similarity
Binding sitei761 – 7611ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 193152UPF1-typeAdd
BLAST
Nucleotide bindingi417 – 4215ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Nonsense-mediated mRNA decay

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase upf1 (EC:3.6.4.-)
Alternative name(s):
Nonsense-mediated mRNA decay protein upf1
Regulator of nonsense transcripts 1 homolog
Up-frameshift suppressor 1
Gene namesi
Name:upf1
ORF Names:SPAC16C9.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC16C9.06c.
PomBaseiSPAC16C9.06c. upf1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 925925ATP-dependent helicase upf1PRO_0000080715Add
BLAST

Proteomic databases

MaxQBiQ09820.
PRIDEiQ09820.

Interactioni

Protein-protein interaction databases

BioGridi278793. 62 interactions.
MINTiMINT-4695411.

Structurei

3D structure databases

ProteinModelPortaliQ09820.
SMRiQ09820. Positions 44-193, 216-836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated
Contains 1 UPF1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 193152UPF1-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000205990.
InParanoidiQ09820.
KOiK14326.
OMAiHDSIGYI.
OrthoDBiEOG70PC5T.
PhylomeDBiQ09820.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR018999. RNA-helicase_UPF1_UPF2-interct.
[Graphical view]
PfamiPF09416. UPF1_Zn_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGLQPNND ISSLVSSKNM TSENGLEHQF EELLVEKQYS EEHCAYCHIK
60 70 80 90 100
NPNSILKCLH CNKWFCNVRG KSGASHIISH LVRARHKQVA LHSHSSLSDT
110 120 130 140 150
VLECYNCGTR NVFLLGFIPA KAKTVVVLLC RQPCARASIA KDMNWDLTQW
160 170 180 190 200
QPIISDRQFL PWLITPPSEE EQKLAIPITS QQMVRLEELW RKDPNANLED
210 220 230 240 250
LDKPIEDDSL PSVELRYKDA HAYQAVLSPL IQAEADYDKR LKESQTQKDV
260 270 280 290 300
VVRWDQAINK RYTAWFLLPK LESGEIRLAI GDEMKLTYEG ELRAPWSSTG
310 320 330 340 350
YVIKIPNNVS DEVGLELKRS DKVPIECTHN FSVDYVWKST SFDRMQTALR
360 370 380 390 400
LFATDGSRLS SFLYHKLLGH DIPPSFLKPK LPSDLSVPNL PKLNASQSEA
410 420 430 440 450
VRAVLSKPLS LIQGPPGTGK TVTSASVVYH LATMQSRKRK SHSPVLVCAP
460 470 480 490 500
SNVAVDQLAE KIHRTGLRVV RVAAKSREDI ESSVSFLSLH EQIKNYKFNP
510 520 530 540 550
ELQRLLKLRS ENNELSIQDE KKLRILVAAA EKELLRAAHV ICCTCVGAGD
560 570 580 590 600
RRISKYKFRS VLIDEATQAS EPECMIPLVL GAKQVVLVGD HQQLGPVVMN
610 620 630 640 650
KKVALASLSQ SLFERLIILG NSPFRLVVQY RMHPCLSEFP SNTFYEGTLQ
660 670 680 690 700
NGVTTSERIA RHVDFPWIQP DSPLMFYANF GQEELSASGT SFLNRTEAST
710 720 730 740 750
CEKIVTTFLR SNVLPEQIGI VTPYDGQRSY IVQYMQNNGS MQKDLYKAVE
760 770 780 790 800
VASVDAFQGR EKDFIILSCV RSSEHQGIGF VNDPRRLNVA LTRAKYGVIV
810 820 830 840 850
LGNPKVLAKH ALWYHFVLHC KEKGYLVEGT LNSLQKFSLT LTPPQKPQKF
860 870 880 890 900
KRDLNVQRSL SPIQNAGSAM LPSFSNLPNL YSSSYLEEWN VFAQYKRRES
910 920
NATDFEDFRS QVGDDESKFD EPTRF
Length:925
Mass (Da):104,529
Last modified:August 14, 2001 - v2
Checksum:i4A5D63C82826E864
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91194.2.
PIRiS62476.
RefSeqiNP_593080.1. NM_001018477.2.

Genome annotation databases

EnsemblFungiiSPAC16C9.06c.1; SPAC16C9.06c.1:pep; SPAC16C9.06c.
GeneIDi2542327.
KEGGispo:SPAC16C9.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA91194.2.
PIRiS62476.
RefSeqiNP_593080.1. NM_001018477.2.

3D structure databases

ProteinModelPortaliQ09820.
SMRiQ09820. Positions 44-193, 216-836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278793. 62 interactions.
MINTiMINT-4695411.

Proteomic databases

MaxQBiQ09820.
PRIDEiQ09820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC16C9.06c.1; SPAC16C9.06c.1:pep; SPAC16C9.06c.
GeneIDi2542327.
KEGGispo:SPAC16C9.06c.

Organism-specific databases

EuPathDBiFungiDB:SPAC16C9.06c.
PomBaseiSPAC16C9.06c. upf1.

Phylogenomic databases

HOGENOMiHOG000205990.
InParanoidiQ09820.
KOiK14326.
OMAiHDSIGYI.
OrthoDBiEOG70PC5T.
PhylomeDBiQ09820.

Enzyme and pathway databases

ReactomeiR-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi20803388.
PROiQ09820.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR018999. RNA-helicase_UPF1_UPF2-interct.
[Graphical view]
PfamiPF09416. UPF1_Zn_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRENT1_SCHPO
AccessioniPrimary (citable) accession number: Q09820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 14, 2001
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.