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Protein

ATP-dependent DNA helicase hus2/rqh1

Gene

rqh1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the repair of UV-induced DNA damage in G2 via recombination-mediated repair. Also has a role in the repair of infrared-induced double DNA strand breaks. Exhibits an ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi555 – 562ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: PomBase
  • DNA duplex unwinding Source: PomBase
  • double-strand break repair via homologous recombination Source: PomBase
  • intra-S DNA damage checkpoint Source: PomBase
  • maintenance of rDNA Source: PomBase
  • mitotic DNA replication DNA duplex unwinding Source: PomBase
  • mitotic recombination-dependent replication fork processing Source: PomBase
  • postreplication repair Source: PomBase
  • recombinational repair Source: PomBase
  • regulation of mitotic recombination involved in replication fork processing Source: PomBase
  • replication fork processing at rDNA locus Source: PomBase
  • resolution of mitotic recombination intermediates Source: PomBase
  • telomere maintenance Source: PomBase
  • UV-damage excision repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-3108214. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase hus2/rqh1 (EC:3.6.4.12)
Gene namesi
Name:rqh1
Synonyms:hus2, rad12, rec9
ORF Names:SPAC2G11.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC2G11.12.
PomBaseiSPAC2G11.12. rqh1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nuclear chromosome Source: PomBase
  • nucleolus Source: PomBase
  • RecQ helicase-Topo III complex Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi543T → I: Loss of helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002050581 – 1328ATP-dependent DNA helicase hus2/rqh1Add BLAST1328

Proteomic databases

MaxQBiQ09811.
PRIDEiQ09811.

Interactioni

Subunit structurei

Interacts with top3.1 Publication

Protein-protein interaction databases

BioGridi278117. 86 interactors.
DIPiDIP-61056N.

Structurei

3D structure databases

ProteinModelPortaliQ09811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini528 – 707Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST180
Domaini728 – 876Helicase C-terminalPROSITE-ProRule annotationAdd BLAST149
Domaini1115 – 1195HRDCPROSITE-ProRule annotationAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi651 – 654DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi225 – 236Poly-AsnAdd BLAST12
Compositional biasi321 – 332Poly-AsnAdd BLAST12

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HRDC domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ09811.
KOiK10901.
OrthoDBiEOG092C0HS8.
PhylomeDBiQ09811.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00614. recQ_fam. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTKTNLNR HLDWFFRESP QKIENVTSPI KTLDFVKVKV SSSDIVVKDS
60 70 80 90 100
IPHKSKNVFD DFDDGYAIDL TEEHQSSSLN NLKWKDVEGP NILKPIKKIA
110 120 130 140 150
VPASESEEDF DDVDEEMLRA AEMEVFQSCQ PLAVNTADTT VSHSTSSSNV
160 170 180 190 200
PRSLNKIHDP SRFIKDNDVE NRIHVSSASK VASISNTSKP NPIVSENPIS
210 220 230 240 250
ATSVSIEIPI KPKELSNNLP FPRLNNNNTN NNNDNNAIEK RDSASPTPSS
260 270 280 290 300
VSSQISIDFS TWPHQNLLQY LDILRDEKSE ISDRIIEVME RYPFSSRFKE
310 320 330 340 350
WIPKRDILSQ KISSVLEVLS NNNNSNNNNG NNGTVPNAKT FFTPPSSITQ
360 370 380 390 400
QVPFPSTIIP ESTVKENSTR PYVNSHLVAN DKITATPFHS EAVVSPLQSN
410 420 430 440 450
IRNSDIAEFD EFDIDDADFT FNTTDPINDE SGASSDVVVI DDEEDDIENR
460 470 480 490 500
PLNQALKASK AAVSNASLLQ SSSLDRPLLG EMKDKNHKVL MPSLDDPMLS
510 520 530 540 550
YPWSKEVLGC LKHKFHLKGF RKNQLEAING TLSGKDVFIL MPTGGGKSLC
560 570 580 590 600
YQLPAVIEGG ASRGVTLVIS PLLSLMQDQL DHLRKLNIPS LPLSGEQPAD
610 620 630 640 650
ERRQVISFLM AKNVLVKLLY VTPEGLASNG AITRVLKSLY ERKLLARIVI
660 670 680 690 700
DEAHCVSHWG HDFRPDYKQL GLLRDRYQGI PFMALTATAN EIVKKDIINT
710 720 730 740 750
LRMENCLELK SSFNRPNLFY EIKPKKDLYT ELYRFISNGH LHESGIIYCL
760 770 780 790 800
SRTSCEQVAA KLRNDYGLKA WHYHAGLEKV ERQRIQNEWQ SGSYKIIVAT
810 820 830 840 850
IAFGMGVDKG DVRFVIHHSF PKSLEGYYQE TGRAGRDGKP AHCIMFYSYK
860 870 880 890 900
DHVTFQKLIM SGDGDAETKE RQRQMLRQVI QFCENKTDCR RKQVLAYFGE
910 920 930 940 950
NFDKVHCRKG CDICCEEATY IKQDMTEFSL QAIKLLKSIS GKATLLQLMD
960 970 980 990 1000
IFRGSKSAKI VENGWDRLEG AGVGKLLNRG DSERLFHHLV SEGVFVEKVE
1010 1020 1030 1040 1050
ANRRGFVSAY VVPGRQTIIN SVLAGKRRII LDVKESSSKP DTSSRSLSRS
1060 1070 1080 1090 1100
KTLPALREYQ LKSTTASVDC SIGTREVDEI YDSQMPPVKP SLIHSRNKID
1110 1120 1130 1140 1150
LEELSGQKFM SEYEIDVMTR CLKDLKLLRS NLMAIDDSRV SSYFTDSVLL
1160 1170 1180 1190 1200
SMAKKLPRNV KELKEIHGVS NEKAVNLGPK FLQVIQKFID EKEQNLEGTE
1210 1220 1230 1240 1250
LDPSLQSLDT DYPIDTNALS LDHEQGFSDD SDSVYEPSSP IEEGDEEVDG
1260 1270 1280 1290 1300
QRKDILNFMN SQSLTQTGSV PKRKSTSYTR PSKSYRHKRG STSYSRKRKY
1310 1320
STSQKDSRKT SKSANTSFIH PMVKQNYR
Length:1,328
Mass (Da):149,646
Last modified:November 1, 1995 - v1
Checksum:i13A9A5B94E5A9BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09426 Genomic DNA. Translation: CAA70577.1.
CU329670 Genomic DNA. Translation: CAA91177.1.
PIRiS62467.
RefSeqiNP_593092.1. NM_001018490.2.

Genome annotation databases

EnsemblFungiiSPAC2G11.12.1; SPAC2G11.12.1:pep; SPAC2G11.12.
GeneIDi2541620.
KEGGispo:SPAC2G11.12.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09426 Genomic DNA. Translation: CAA70577.1.
CU329670 Genomic DNA. Translation: CAA91177.1.
PIRiS62467.
RefSeqiNP_593092.1. NM_001018490.2.

3D structure databases

ProteinModelPortaliQ09811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278117. 86 interactors.
DIPiDIP-61056N.

Proteomic databases

MaxQBiQ09811.
PRIDEiQ09811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC2G11.12.1; SPAC2G11.12.1:pep; SPAC2G11.12.
GeneIDi2541620.
KEGGispo:SPAC2G11.12.

Organism-specific databases

EuPathDBiFungiDB:SPAC2G11.12.
PomBaseiSPAC2G11.12. rqh1.

Phylogenomic databases

InParanoidiQ09811.
KOiK10901.
OrthoDBiEOG092C0HS8.
PhylomeDBiQ09811.

Enzyme and pathway databases

ReactomeiR-SPO-3108214. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

PROiQ09811.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00614. recQ_fam. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHUS2_SCHPO
AccessioniPrimary (citable) accession number: Q09811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.