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Protein

Suppressor protein of bem1/bed5 double mutants

Gene

vps4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Involved in multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1768ATPSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: PomBase
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. late endosome to vacuole transport Source: PomBase
  2. protein retention in Golgi apparatus Source: PomBase
  3. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: PomBase
  4. vacuole organization Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Suppressor protein of bem1/bed5 double mutants (EC:3.6.4.6)
Gene namesi
Name:vps4
ORF Names:SPAC2G11.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC2G11.06.

Subcellular locationi

Endosome membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. endosome membrane Source: UniProtKB-SubCell
  4. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Suppressor protein of bem1/bed5 double mutantsPRO_0000084769Add
BLAST

Proteomic databases

MaxQBiQ09803.

Interactioni

Protein-protein interaction databases

BioGridi278533. 1 interaction.
MINTiMINT-4695267.
STRINGi4896.SPAC2G11.06-1.

Structurei

3D structure databases

ProteinModelPortaliQ09803.
SMRiQ09803. Positions 1-78, 118-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8078MITAdd
BLAST

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated
Contains 1 MIT domain.Curated

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000225146.
InParanoidiQ09803.
KOiK12196.
OMAiFRLYQHA.
OrthoDBiEOG72JWRT.
PhylomeDBiQ09803.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNPDCLSKA ISLVKTAIDN DNAEQYPDAY KYYQSALDYF MMALKYEKNE
60 70 80 90 100
KSKEIIRSKV IEYLDRAEKL KVYLQEKNNQ ISSKSRVSNG NVEGSNSPTA
110 120 130 140 150
NEALDSDAKK LRSALTSAIL VEKPNVRWDD IAGLENAKEA LKETVLLPIK
160 170 180 190 200
LPQLFSHGRK PWSGILLYGP PGTGKSYLAK AVATEAGSTF FSISSSDLVS
210 220 230 240 250
KWMGESERLV RQLFEMAREQ KPSIIFIDEI DSLCGSRSEG ESESSRRIKT
260 270 280 290 300
EFLVQMNGVG KDESGVLVLG ATNIPWTLDS AIRRRFEKRI YIPLPNAHAR
310 320 330 340 350
ARMFELNVGK IPSELTSQDF KELAKMTDGY SGSDISIVVR DAIMEPVRRI
360 370 380 390 400
HTATHFKEVY DNKSNRTLVT PCSPGDPDAF ESSWLEVNPE DIMEPKLTVR
410 420 430
DFYSAVRKVK PTLNAGDIEK HTQFTKDFGA EG
Length:432
Mass (Da):48,402
Last modified:November 1, 1995 - v1
Checksum:i65E6948AB9360D1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33456 Genomic DNA. Translation: AAA35347.1.
CU329670 Genomic DNA. Translation: CAA91171.1.
PIRiT38572. S62461.
RefSeqiNP_593086.1. NM_001018484.2.

Genome annotation databases

EnsemblFungiiSPAC2G11.06.1; SPAC2G11.06.1:pep; SPAC2G11.06.
GeneIDi2542054.
KEGGispo:SPAC2G11.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33456 Genomic DNA. Translation: AAA35347.1.
CU329670 Genomic DNA. Translation: CAA91171.1.
PIRiT38572. S62461.
RefSeqiNP_593086.1. NM_001018484.2.

3D structure databases

ProteinModelPortaliQ09803.
SMRiQ09803. Positions 1-78, 118-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278533. 1 interaction.
MINTiMINT-4695267.
STRINGi4896.SPAC2G11.06-1.

Proteomic databases

MaxQBiQ09803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC2G11.06.1; SPAC2G11.06.1:pep; SPAC2G11.06.
GeneIDi2542054.
KEGGispo:SPAC2G11.06.

Organism-specific databases

PomBaseiSPAC2G11.06.

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000225146.
InParanoidiQ09803.
KOiK12196.
OMAiFRLYQHA.
OrthoDBiEOG72JWRT.
PhylomeDBiQ09803.

Miscellaneous databases

NextBioi20803129.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Valencik M.L., Pringle J.R.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiVPS4_SCHPO
AccessioniPrimary (citable) accession number: Q09803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.