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Protein

PAN2-PAN3 deadenylation complex catalytic subunit pan2

Gene

pan2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein pab1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by xrn1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 2 metal cations per subunit in the catalytic exonuclease domain.UniRule annotation

Enzyme regulationi

Positively regulated by the regulatory subunit ppk26/pan3.UniRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi863Divalent metal cation; catalyticUniRule annotation1
Metal bindingi865Divalent metal cation; catalyticUniRule annotation1
Metal bindingi972Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1025Divalent metal cation; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processmRNA processing
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PAN2-PAN3 deadenylation complex catalytic subunit pan2UniRule annotation (EC:3.1.13.4UniRule annotation)
Alternative name(s):
PAB1P-dependent poly(A)-specific ribonucleaseUniRule annotation
Poly(A)-nuclease deadenylation complex subunit 2UniRule annotation
Short name:
PAN deadenylation complex subunit 2UniRule annotation
Gene namesi
Name:pan2UniRule annotation
Synonyms:ubp13
ORF Names:SPAC22G7.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22G7.04
PomBaseiSPAC22G7.04 pan2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001164061 – 1088PAN2-PAN3 deadenylation complex catalytic subunit pan2Add BLAST1088

Proteomic databases

MaxQBiQ09798
PaxDbiQ09798
PRIDEiQ09798

PTM databases

iPTMnetiQ09798

Interactioni

Subunit structurei

Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit ppk26/pan3 to form the poly(A)-nuclease (PAN) deadenylation complex.UniRule annotation

Protein-protein interaction databases

BioGridi278180, 1 interactor
STRINGi4896.SPAC22G7.04.1

Structurei

3D structure databases

SMRiQ09798
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati16 – 56WD 1UniRule annotationAdd BLAST41
Repeati136 – 175WD 2UniRule annotationAdd BLAST40
Repeati178 – 224WD 3UniRule annotationAdd BLAST47
Repeati270 – 309WD 4UniRule annotationAdd BLAST40
Domaini443 – 814USPUniRule annotationAdd BLAST372
Domaini860 – 1033ExonucleaseUniRule annotationAdd BLAST174

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 443LinkerUniRule annotationAdd BLAST135

Domaini

Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.UniRule annotation
The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with ppk26/pan3.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. PAN2 subfamily.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000230585
InParanoidiQ09798
KOiK12571
OMAiIHLWGSP
OrthoDBiEOG092C0KHR

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.420.10, 1 hit
HAMAPiMF_03182 PAN2, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR030843 PAN2
IPR028881 PAN2_dom
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR028889 USP_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
PF13423 UCH_1, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q09798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAWKEITKT TENDGVSTCS LSSIAFDPYS ELVWTGHKNG QIKSSFGPSL
60 70 80 90 100
TSYTQFIGHE GPVHQVLPQE RGVFSLSSKS LRLSNRKGTI RWRYQDSDCI
110 120 130 140 150
DYRAMFYQSR NNPEVVIGGY HQKLTVVNAE RGISIHKDKN VSDIFIMRRN
160 170 180 190 200
RLLCCGSTNG EIILRDPNSF QPVNKVVAHT GTISDIDTSG NLLLSCGYSL
210 220 230 240 250
RHGTYMLDPF VKVWDLRNLS SLVPIPFPAG PTIIRMHPKL STTAVVCSCS
260 270 280 290 300
GQFHIVDTGN PLDAKLMQIP LTSYLTGMDI ASTGDAMVFT DVEDNIHLWS
310 320 330 340 350
PLENPSFSDL KLPIQLPNTS TETVQLENND PLNSIGLPYY KDELLSSWSK
360 370 380 390 400
YLIFDVGKPI LDSNLLIAKQ ISENSHPVPQ EIKSFHRNQI IEVPWLNRKL
410 420 430 440 450
ISEGATPKFH SERQKDIMSG NDIEGSASYF EEIEDTISGP DSIPKFYQRP
460 470 480 490 500
VIKYSKFGIE DFDFGFYNKT KYAGLETDIT NSYCNSVLQL LSYVPSFSKA
510 520 530 540 550
AISHSLGPCD LMECLLCELG FLFAMLKEST GRNCQATNFL RAFSNSSFAQ
560 570 580 590 600
SLGIVFDDYS DGTFPDSFVI QKFTKFMLTE ISRIADYEDK KDGTSFPVSF
610 620 630 640 650
LLKSFCIPEM QTYRCGICGI TSQKIKSSLY IIDLHYPSQQ LESILSFEWL
660 670 680 690 700
FKMSLDRRVD LPPGWCEYCL AHQPFLLRSF IRSLPDCLFI NTQVKHHEHW
710 720 730 740 750
KLWARKNWLP KKLHLRRVND TMQCVSQKIS NLDKDQQSLS VYVLRGIIYE
760 770 780 790 800
IRQNGEEPHF VSTIRVSDNT SSDNPDDNRW YIFNDFLVKE VTEEEALTVH
810 820 830 840 850
GPWKIPIIVY YEKLDTKIPQ WDEVSDYTLL YQPYSLNKNP PINKIQPLTT
860 870 880 890 900
DEMLYPKMLV GIDSEFVALQ QEETEVRSDG TKSTIKPSKL SLARVSVLRG
910 920 930 940 950
EGPNKGLPFI DDYVATDDKV TDYLTEYSGI HPGDLDPDRS PYNVVPLKVA
960 970 980 990 1000
YKKLRLLVNA GCIFVGHGLQ KDFRIINLLV PPEQVVDTVD LFFLSSRQRK
1010 1020 1030 1040 1050
LSLKFLAWYL LDEEIQLTEH DSIEDALTAL KLYDCYDKLK SQGKLEETLD
1060 1070 1080
NIYEVGRRFK FRPPSVASMS LEDRNSYGDE SVISNQTN
Length:1,088
Mass (Da):123,874
Last modified:March 21, 2012 - v2
Checksum:i7412DA56B49D6EDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA91128.2
PIRiT11614
RefSeqiNP_593053.2, NM_001018451.2

Genome annotation databases

EnsemblFungiiSPAC22G7.04.1; SPAC22G7.04.1:pep; SPAC22G7.04
GeneIDi2541684
KEGGispo:SPAC22G7.04

Similar proteinsi

Entry informationi

Entry nameiPAN2_SCHPO
AccessioniPrimary (citable) accession number: Q09798
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: March 21, 2012
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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