Q09768 (GSH1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gcs1
ORF Names:	SPAC22F3.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Subunit structure	Heterodimer of a catalytic heavy chain and a regulatory light chain By similarity.
Sequence similarities	Belongs to the glutamate--cysteine ligase type 3 family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to cadmium ion Inferred from mutant phenotype. Source: GeneDB_Spombe glutathione biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe glutamate-cysteine ligase complex Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from mutant phenotype. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Glutamate--cysteine ligase		PRO_0000192571
Experimental info
	Sequence conflict	28	1	M → I Ref.1
	Sequence conflict	79 – 123	45	Missing in CAA59379. Ref.1
	Sequence conflict	105	1	K → N in BAA09527. Ref.2
	Sequence conflict	399	1	E → A in BAA09527. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q09768 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: CE676112B1FB768C	FASTA	669	76,523
	10 20 30 40 50 60 MGLLVLGTPL DWPESKKYCD YVRENGIMQF LHMYDTYISK KQDVLLWGDE IECIVVSMDD 70 80 90 100 110 120 KSKKARVSLR QEDILNALGK YEETFRHVDF GPVYAALRNE TCPKKIDAIL SEVAKNPADY 130 140 150 160 170 180 VERIGGNSNK DTIEITSSTK PHAQNAVPTF HPEYGRYMLE STPGAPYGST LKDFTFVEYN 190 200 210 220 230 240 MRLRRKIIEN HLLPNELPLT ITNFFRLGTP GFTDPEVEAN GAISRSFFLP DDVINTHVRF 250 260 270 280 290 300 PTLTANIRQR RGRKVAMNVP IFFDKNTIKP FHDPTVPWDR NLFPEDANAR DGAALDNHIY 310 320 330 340 350 360 MDSMGFGMGC CCLQITFQAK SCDEARLLYD QLTPITPLML ALSAGTPAFR GYLADQDCRW 370 380 390 400 410 420 NVIAGAVDDR TEEEMKTVPK SRYDSVDLYI SNDKRNLPEY NDVPVVINQD CYDKLIKDCI 430 440 450 460 470 480 DERLAKHMAH IFSRDPLVIF SDSILQDNSV SNAHFENLNS TNWQSMRFKP PPPGSDIGWR 490 500 510 520 530 540 VEFRSMEIQI TDFENAAYSI FVVMLSRAIL SFNLNLYMPI SLVDENMKAA HARDAIHRKK 550 560 570 580 590 600 FWFRCNPFPD ASTDDESGQF RQLTIDELFN GEHRENGFPG LITIVRSYLY SCNPDAKTIC 610 620 630 640 650 660 LIERYIRLIS QRANGQCLTA ASWIRNFITT HPSYKQDSVV NDEINYDLIR RIAKIVDGDY DDTLLGKCS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gcs1, a gene encoding gamma-glutamylcysteine synthetase in the fission yeast Schizosaccharomyces pombe." Coblenz A., Wolf K. Yeast 11:1171-1177(1995) [PubMed: 8619315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D18.
[2]	"Molecular cloning and nucleotide sequencing of the gamma-glutamylcysteine synthetase gene of the fission yeast Schizosaccharomyces pombe." Mutoh N. J. Biochem. 117:283-288(1995) [PubMed: 7608113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85017 Genomic DNA. Translation: CAA59379.1. D55676 Genomic DNA. Translation: BAA09527.1. CU329670 Genomic DNA. Translation: CAA91075.1.
PIR	T38181.
RefSeq	NP_593031.1. NM_001018430.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q09768.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC22F3.10c.1; SPAC22F3.10c.1:pep; SPAC22F3.10c.
GeneID	2541881.
GenomeReviews	Gene locus gcs1 in contig CU329670_GR.
KEGG	spo:SPAC22F3.10c.
NMPDR	fig|4896.1.peg.3001.
Organism-specific databases
GeneDB_Spombe	SPAC22F3.10c.
Phylogenomic databases
eggNOG	fuNOG05294.
GeneTree	EFGT00050000004278.
HOGENOM	HBG331200.
OMA	QVTFQAC.
OrthoDB	EOG4S1XGD.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000751-MONOMER.
BRENDA	6.3.2.2. 5615.
Gene expression databases
ArrayExpress	Q09768.
Family and domain databases
InterPro	IPR004308. GCS. IPR016027. NA-bd_OB-fold-like. [Graphical view]
KO	K11204.
PANTHER	PTHR11164. GCS. 1 hit.
Pfam	PF03074. GCS. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_SCHPO

Accession

Primary (citable) accession number: Q09768

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program