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Protein

Glutamate--cysteine ligase

Gene

gcs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative glutamate--cysteine ligase regulatory subunit (SPCC737.06c), Glutamate--cysteine ligase (gcs1)
  2. Glutathione synthetase large chain (gsa1)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-cysteine ligase activity Source: PomBase

GO - Biological processi

  • cellular detoxification of cadmium ion Source: PomBase
  • cellular response to cadmium ion Source: PomBase
  • glutathione biosynthetic process Source: PomBase
  • glutathione transport Source: PomBase
  • phytochelatin biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.2. 5613.
ReactomeiR-SPO-1614635. Sulfur amino acid metabolism.
R-SPO-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Gene namesi
Name:gcs1
ORF Names:SPAC22F3.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22F3.10c.
PomBaseiSPAC22F3.10c. gcs1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • glutamate-cysteine ligase complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 669669Glutamate--cysteine ligasePRO_0000192571Add
BLAST

Proteomic databases

MaxQBiQ09768.

Interactioni

Subunit structurei

Heterodimer of a catalytic heavy chain and a regulatory light chain.By similarity

Protein-protein interaction databases

MINTiMINT-4694878.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000199354.
InParanoidiQ09768.
KOiK11204.
OMAiPIYRGYL.
OrthoDBiEOG773XQR.
PhylomeDBiQ09768.

Family and domain databases

InterProiIPR004308. GCS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 2 hits.
PfamiPF03074. GCS. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLVLGTPL DWPESKKYCD YVRENGIMQF LHMYDTYISK KQDVLLWGDE
60 70 80 90 100
IECIVVSMDD KSKKARVSLR QEDILNALGK YEETFRHVDF GPVYAALRNE
110 120 130 140 150
TCPKKIDAIL SEVAKNPADY VERIGGNSNK DTIEITSSTK PHAQNAVPTF
160 170 180 190 200
HPEYGRYMLE STPGAPYGST LKDFTFVEYN MRLRRKIIEN HLLPNELPLT
210 220 230 240 250
ITNFFRLGTP GFTDPEVEAN GAISRSFFLP DDVINTHVRF PTLTANIRQR
260 270 280 290 300
RGRKVAMNVP IFFDKNTIKP FHDPTVPWDR NLFPEDANAR DGAALDNHIY
310 320 330 340 350
MDSMGFGMGC CCLQITFQAK SCDEARLLYD QLTPITPLML ALSAGTPAFR
360 370 380 390 400
GYLADQDCRW NVIAGAVDDR TEEEMKTVPK SRYDSVDLYI SNDKRNLPEY
410 420 430 440 450
NDVPVVINQD CYDKLIKDCI DERLAKHMAH IFSRDPLVIF SDSILQDNSV
460 470 480 490 500
SNAHFENLNS TNWQSMRFKP PPPGSDIGWR VEFRSMEIQI TDFENAAYSI
510 520 530 540 550
FVVMLSRAIL SFNLNLYMPI SLVDENMKAA HARDAIHRKK FWFRCNPFPD
560 570 580 590 600
ASTDDESGQF RQLTIDELFN GEHRENGFPG LITIVRSYLY SCNPDAKTIC
610 620 630 640 650
LIERYIRLIS QRANGQCLTA ASWIRNFITT HPSYKQDSVV NDEINYDLIR
660
RIAKIVDGDY DDTLLGKCS
Length:669
Mass (Da):76,523
Last modified:November 1, 1995 - v1
Checksum:iCE676112B1FB768C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281M → I (PubMed:8619315).Curated
Sequence conflicti79 – 12345Missing in CAA59379 (PubMed:8619315).CuratedAdd
BLAST
Sequence conflicti105 – 1051K → N in BAA09527 (PubMed:7608113).Curated
Sequence conflicti399 – 3991E → A in BAA09527 (PubMed:7608113).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85017 Genomic DNA. Translation: CAA59379.1.
D55676 Genomic DNA. Translation: BAA09527.1.
CU329670 Genomic DNA. Translation: CAA91075.1.
PIRiT38181.
RefSeqiNP_593031.1. NM_001018430.2.

Genome annotation databases

EnsemblFungiiSPAC22F3.10c.1; SPAC22F3.10c.1:pep; SPAC22F3.10c.
GeneIDi2541881.
KEGGispo:SPAC22F3.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85017 Genomic DNA. Translation: CAA59379.1.
D55676 Genomic DNA. Translation: BAA09527.1.
CU329670 Genomic DNA. Translation: CAA91075.1.
PIRiT38181.
RefSeqiNP_593031.1. NM_001018430.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4694878.

Proteomic databases

MaxQBiQ09768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC22F3.10c.1; SPAC22F3.10c.1:pep; SPAC22F3.10c.
GeneIDi2541881.
KEGGispo:SPAC22F3.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC22F3.10c.
PomBaseiSPAC22F3.10c. gcs1.

Phylogenomic databases

HOGENOMiHOG000199354.
InParanoidiQ09768.
KOiK11204.
OMAiPIYRGYL.
OrthoDBiEOG773XQR.
PhylomeDBiQ09768.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
BRENDAi6.3.2.2. 5613.
ReactomeiR-SPO-1614635. Sulfur amino acid metabolism.
R-SPO-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiQ09768.

Family and domain databases

InterProiIPR004308. GCS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 2 hits.
PfamiPF03074. GCS. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gcs1, a gene encoding gamma-glutamylcysteine synthetase in the fission yeast Schizosaccharomyces pombe."
    Coblenz A., Wolf K.
    Yeast 11:1171-1177(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D18.
  2. "Molecular cloning and nucleotide sequencing of the gamma-glutamylcysteine synthetase gene of the fission yeast Schizosaccharomyces pombe."
    Mutoh N.
    J. Biochem. 117:283-288(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiGSH1_SCHPO
AccessioniPrimary (citable) accession number: Q09768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.