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Protein

NEDD8-activating enzyme E1 catalytic subunit

Gene

uba3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the dimeric uba3-ula1 E1 enzyme. E1 activates NEDD8/ubl1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uba3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc12 (By similarity).By similarity

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei205 – 2051Glycyl thioester intermediatePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi54 – 7825ATPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-activating enzyme E1 catalytic subunit (EC:6.3.2.-)
Alternative name(s):
Ubiquitin-activating enzyme E1 3
Gene namesi
Name:uba3
ORF Names:SPAC24H6.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.12c.
PomBaseiSPAC24H6.12c. uba3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444NEDD8-activating enzyme E1 catalytic subunitPRO_0000194949Add
BLAST

Proteomic databases

MaxQBiQ09765.

Interactioni

Subunit structurei

Heterodimer of uba3 and ula1. Interacts with NEDD8 and ubc12 (By similarity). Interacts with but1 and but2.By similarity1 Publication

Protein-protein interaction databases

BioGridi278575. 5 interactions.
MINTiMINT-4694838.

Structurei

3D structure databases

ProteinModelPortaliQ09765.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000166793.
InParanoidiQ09765.
KOiK10686.
OMAiDESYYMQ.
OrthoDBiEOG092C3FGE.
PhylomeDBiQ09765.

Family and domain databases

Gene3Di1.10.10.520. 1 hit.
3.10.20.260. 1 hit.
3.40.50.720. 2 hits.
InterProiIPR014929. E2_binding.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR023318. Ub_act_enz_dom_a.
IPR030468. Uba3.
IPR033127. UBQ-activ_enz_E1_Cys_AS.
[Graphical view]
PANTHERiPTHR10953:SF6. PTHR10953:SF6. 2 hits.
PfamiPF08825. E2_bind. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM01181. E2_bind. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSDVCKAG SHRHSGWIQS LKKPGPFNLD APENPEETLK SAFSSKILII
60 70 80 90 100
GAGGLGCEIL KDLALSGFRD LSVIDMDTID ITNLNRQFLF NESNIDEPKA
110 120 130 140 150
NVAASMIMKR IPSTVVTPFY GKIQDKTIEF YKEFKLIICG LDSVEARRWI
160 170 180 190 200
NSTLVAIAKT GDLIPLVDGG SEGLKGQARV IIPTITSCYE CSLDMLTPKI
210 220 230 240 250
SYPICTLANT PRLPEHCVEW AYLLEWPRVF LNASVDSFSK QEVFEPLDGK
260 270 280 290 300
NSNFEPDNIR HIDWLVKRSI ERANKFQIPS SSINRFFVQG IVKRIIPAVA
310 320 330 340 350
STNAIIAASC CNEALKILTE SNPFLDNYMM YVGEDGAYTY TFNLEKRSDC
360 370 380 390 400
PVCGVLSEVY DISASSTVTL KDILNHYSKS YNLQNPSVST AAGTPLYLAS
410 420 430 440
PPALQVATSK NLSQPILSIT SVDVNLVITD KNLSTSLSVQ LREC
Length:444
Mass (Da):48,942
Last modified:November 1, 1995 - v1
Checksum:iD1734FE0DA61117D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90856.1.
PIRiT38368. S62414.
RefSeqiNP_592940.1. NM_001018341.2.

Genome annotation databases

EnsemblFungiiSPAC24H6.12c.1; SPAC24H6.12c.1:pep; SPAC24H6.12c.
GeneIDi2542099.
KEGGispo:SPAC24H6.12c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90856.1.
PIRiT38368. S62414.
RefSeqiNP_592940.1. NM_001018341.2.

3D structure databases

ProteinModelPortaliQ09765.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278575. 5 interactions.
MINTiMINT-4694838.

Proteomic databases

MaxQBiQ09765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC24H6.12c.1; SPAC24H6.12c.1:pep; SPAC24H6.12c.
GeneIDi2542099.
KEGGispo:SPAC24H6.12c.

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.12c.
PomBaseiSPAC24H6.12c. uba3.

Phylogenomic databases

HOGENOMiHOG000166793.
InParanoidiQ09765.
KOiK10686.
OMAiDESYYMQ.
OrthoDBiEOG092C3FGE.
PhylomeDBiQ09765.

Enzyme and pathway databases

UniPathwayiUPA00885.

Miscellaneous databases

PROiQ09765.

Family and domain databases

Gene3Di1.10.10.520. 1 hit.
3.10.20.260. 1 hit.
3.40.50.720. 2 hits.
InterProiIPR014929. E2_binding.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR023318. Ub_act_enz_dom_a.
IPR030468. Uba3.
IPR033127. UBQ-activ_enz_E1_Cys_AS.
[Graphical view]
PANTHERiPTHR10953:SF6. PTHR10953:SF6. 2 hits.
PfamiPF08825. E2_bind. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM01181. E2_bind. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBA3_SCHPO
AccessioniPrimary (citable) accession number: Q09765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.