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Protein

Cullin-3

Gene

cul3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable core component of multiple cullin-RING-based BC3B (BTB-CUL3-BTB) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the BC3B complex depends on the substrate recognition component (By similarity). Involved in ubiquitin-mediated degradation of btb3.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-5632684 Hedgehog 'on' state
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-3
Short name:
Cul-3
Gene namesi
Name:cul3
Synonyms:pcu3
ORF Names:SPAC24H6.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.03
PomBaseiSPAC24H6.03 cul3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50Y → G: Reduced btb3 binding and ubiquitination. 1 Publication1
Mutagenesisi729K → R: No cross-link with NEDD8. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198091 – 785Cullin-3Add BLAST785

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki729Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated; enhancing the ubiquitin-ligase activity.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ09760
PaxDbiQ09760
PRIDEiQ09760

Interactioni

Subunit structurei

Probable component of multiple cullin-RING-based BC3B (BTB-CUL3-BTB) E3 ubiquitin-protein ligase complexes formed by cul-3, rbx-1 and a variable BTB domain-containing protein as adapter and substrate recognition component (By similarity). Interacts with btb1, btb2, btb3, nedd8 and pip1.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279091, 20 interactors
IntActiQ09760, 14 interactors
STRINGi4896.SPAC24H6.03.1

Structurei

3D structure databases

ProteinModelPortaliQ09760
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000176712
InParanoidiQ09760
KOiK03869
OMAiTSTFWPM
OrthoDBiEOG092C1L4K
PhylomeDBiQ09760

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 2 hits
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q09760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRSAKLKIR APRKFSANQV DFATHWEVLQ RAIGDIFQKS TSQLSFEELY
60 70 80 90 100
RNAYILVLHK YGEKLYNHVQ DVIRSRLKEE TVPAIYKNYD ASLLGNALLD
110 120 130 140 150
IRKNDSYSTS WSRSLEAAHR FLSSLVNSWK DHIVSMQMIS SVLKYLDKVY
160 170 180 190 200
SKSADKVPVN ENGIYIFREV VLLNSFEIGE KCVETILILV YLERKGNTIN
210 220 230 240 250
RPLINDCLDM LNSLPSENKK ETLYDVLFAP KFLSYTRNFY EIESSTVIGV
260 270 280 290 300
FGVVEYLKKA EKRFEEEKER SKNYLFTKIA SPLLSVVEDE LLSKHLDDLL
310 320 330 340 350
ENQSTGFFSM IDSSNFEGLQ LVYESFSRVE LGVKSLKKYL AKYVAHHGKL
360 370 380 390 400
INETTSQALE GKMAVGRLSS NATMATLWVQ KVLALWDRLN TIISTTMDAD
410 420 430 440 450
RSILNSLSDA FVTFVDGYTR APEYISLFID DNLKKDARKA IEGSIEATLQ
460 470 480 490 500
NSVTLFRFIS EKDVFEKYYK THLAKRLLNN RSISSDAELG MISRLKQEAG
510 520 530 540 550
NVFTQKLEGM FNDMNLSQEL LQEYKHNSAL QSAKPALDLN VSILASTFWP
560 570 580 590 600
IDLSPHKIKC NFPKVLLAQI DQFTDFYLSK HTGRKLLWYP SMGSADVRVN
610 620 630 640 650
FKDRKYDLNV STIASVILLL FQDLKENQCL IFEEILEKTN IEVGDLKRNL
660 670 680 690 700
QSLACAKYKI LLKDPKGREV NAGDKFYFNE NFVSNLARIK ISTVAQTRVE
710 720 730 740 750
DDSERKRTLE KVDESRKHQA DACIVRVMKD RKVCEHNQLM AEVTRQLNPR
760 770 780
FHPSPMMIKR RIEALIEREY LQRQADNGRI YEYLA
Length:785
Mass (Da):90,512
Last modified:December 15, 1998 - v2
Checksum:i0830F7A857DF6CE7
GO

Sequence cautioni

The sequence BAA32519 differs from that shown. Reason: Frameshift at position 513.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti476R → RYALIVFTVFNTFR in BAA32519 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA90847.1
AB017028 Genomic DNA Translation: BAA32519.1 Frameshift.
PIRiS62405
T38359
T43406
RefSeqiNP_592949.1, NM_001018350.2

Genome annotation databases

EnsemblFungiiSPAC24H6.03.1; SPAC24H6.03.1:pep; SPAC24H6.03
GeneIDi2542637
KEGGispo:SPAC24H6.03

Similar proteinsi

Entry informationi

Entry nameiCUL3_SCHPO
AccessioniPrimary (citable) accession number: Q09760
Secondary accession number(s): O74185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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