Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cullin-3

Gene

cul3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable core component of multiple cullin-RING-based BC3B (BTB-CUL3-BTB) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the BC3B complex depends on the substrate recognition component (By similarity). Involved in ubiquitin-mediated degradation of btb3.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-5632684. Hedgehog 'on' state.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-3
Short name:
Cul-3
Gene namesi
Name:cul3
Synonyms:pcu3
ORF Names:SPAC24H6.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.03.
PomBaseiSPAC24H6.03. cul3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • SCF ubiquitin ligase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi50 – 501Y → G: Reduced btb3 binding and ubiquitination. 1 Publication
Mutagenesisi729 – 7291K → R: No cross-link with NEDD8. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785Cullin-3PRO_0000119809Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki729 – 729Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ09760.

Interactioni

Subunit structurei

Probable component of multiple cullin-RING-based BC3B (BTB-CUL3-BTB) E3 ubiquitin-protein ligase complexes formed by cul-3, rbx-1 and a variable BTB domain-containing protein as adapter and substrate recognition component (By similarity). Interacts with btb1, btb2, btb3, nedd8 and pip1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
btb1O748813EBI-3647930,EBI-3647943
btb2O747782EBI-3647930,EBI-3648173
btb3Q102254EBI-3647930,EBI-3648208

Protein-protein interaction databases

BioGridi279091. 20 interactions.
IntActiQ09760. 14 interactions.
MINTiMINT-4694776.

Structurei

3D structure databases

ProteinModelPortaliQ09760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000176712.
InParanoidiQ09760.
KOiK03869.
OMAiHKIRAPR.
OrthoDBiEOG7FJH80.
PhylomeDBiQ09760.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 2 hits.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRSAKLKIR APRKFSANQV DFATHWEVLQ RAIGDIFQKS TSQLSFEELY
60 70 80 90 100
RNAYILVLHK YGEKLYNHVQ DVIRSRLKEE TVPAIYKNYD ASLLGNALLD
110 120 130 140 150
IRKNDSYSTS WSRSLEAAHR FLSSLVNSWK DHIVSMQMIS SVLKYLDKVY
160 170 180 190 200
SKSADKVPVN ENGIYIFREV VLLNSFEIGE KCVETILILV YLERKGNTIN
210 220 230 240 250
RPLINDCLDM LNSLPSENKK ETLYDVLFAP KFLSYTRNFY EIESSTVIGV
260 270 280 290 300
FGVVEYLKKA EKRFEEEKER SKNYLFTKIA SPLLSVVEDE LLSKHLDDLL
310 320 330 340 350
ENQSTGFFSM IDSSNFEGLQ LVYESFSRVE LGVKSLKKYL AKYVAHHGKL
360 370 380 390 400
INETTSQALE GKMAVGRLSS NATMATLWVQ KVLALWDRLN TIISTTMDAD
410 420 430 440 450
RSILNSLSDA FVTFVDGYTR APEYISLFID DNLKKDARKA IEGSIEATLQ
460 470 480 490 500
NSVTLFRFIS EKDVFEKYYK THLAKRLLNN RSISSDAELG MISRLKQEAG
510 520 530 540 550
NVFTQKLEGM FNDMNLSQEL LQEYKHNSAL QSAKPALDLN VSILASTFWP
560 570 580 590 600
IDLSPHKIKC NFPKVLLAQI DQFTDFYLSK HTGRKLLWYP SMGSADVRVN
610 620 630 640 650
FKDRKYDLNV STIASVILLL FQDLKENQCL IFEEILEKTN IEVGDLKRNL
660 670 680 690 700
QSLACAKYKI LLKDPKGREV NAGDKFYFNE NFVSNLARIK ISTVAQTRVE
710 720 730 740 750
DDSERKRTLE KVDESRKHQA DACIVRVMKD RKVCEHNQLM AEVTRQLNPR
760 770 780
FHPSPMMIKR RIEALIEREY LQRQADNGRI YEYLA
Length:785
Mass (Da):90,512
Last modified:December 15, 1998 - v2
Checksum:i0830F7A857DF6CE7
GO

Sequence cautioni

The sequence BAA32519.1 differs from that shown. Reason: Frameshift at position 513. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti476 – 4761R → RYALIVFTVFNTFR in BAA32519 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90847.1.
AB017028 Genomic DNA. Translation: BAA32519.1. Frameshift.
PIRiS62405.
T38359.
T43406.
RefSeqiNP_592949.1. NM_001018350.2.

Genome annotation databases

EnsemblFungiiSPAC24H6.03.1; SPAC24H6.03.1:pep; SPAC24H6.03.
GeneIDi2542637.
KEGGispo:SPAC24H6.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90847.1.
AB017028 Genomic DNA. Translation: BAA32519.1. Frameshift.
PIRiS62405.
T38359.
T43406.
RefSeqiNP_592949.1. NM_001018350.2.

3D structure databases

ProteinModelPortaliQ09760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279091. 20 interactions.
IntActiQ09760. 14 interactions.
MINTiMINT-4694776.

Proteomic databases

MaxQBiQ09760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC24H6.03.1; SPAC24H6.03.1:pep; SPAC24H6.03.
GeneIDi2542637.
KEGGispo:SPAC24H6.03.

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.03.
PomBaseiSPAC24H6.03. cul3.

Phylogenomic databases

HOGENOMiHOG000176712.
InParanoidiQ09760.
KOiK03869.
OMAiHKIRAPR.
OrthoDBiEOG7FJH80.
PhylomeDBiQ09760.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-SPO-5632684. Hedgehog 'on' state.

Miscellaneous databases

NextBioi20803686.
PROiQ09760.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 2 hits.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Kominami K., Toda T.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 136-543.
  3. "BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases."
    Geyer R., Wee S., Anderson S., Yates J. III, Wolf D.A.
    Mol. Cell 12:783-790(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BTB1; BTB2; BTB3; NEDD8 AND PIP1, MUTAGENESIS OF TYR-50 AND LYS-729.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCUL3_SCHPO
AccessioniPrimary (citable) accession number: Q09760
Secondary accession number(s): O74185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: May 11, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.