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Q09751

- LGUL_SCHPO

UniProt

Q09751 - LGUL_SCHPO

Protein

Lactoylglutathione lyase

Gene

glo1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.1 Publication

    Catalytic activityi

    (R)-S-lactoylglutathione = glutathione + methylglyoxal.

    Cofactori

    Binds 1 zinc ion per subunit. Cobalt, nickel and manganese ions can also function as cofactors.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi14 – 141Zinc; via tele nitrogen; shared with dimeric partnerBy similarity
    Binding sitei18 – 181SubstrateBy similarity
    Metal bindingi75 – 751Zinc; shared with dimeric partnerBy similarity
    Binding sitei79 – 791SubstrateBy similarity
    Binding sitei99 – 991SubstrateBy similarity
    Metal bindingi103 – 1031Zinc; via tele nitrogenBy similarity
    Binding sitei103 – 1031SubstrateBy similarity
    Active sitei149 – 1491Proton donor/acceptorBy similarity
    Metal bindingi149 – 1491ZincBy similarity

    GO - Molecular functioni

    1. lactoylglutathione lyase activity Source: PomBase
    2. zinc ion binding Source: PomBase

    GO - Biological processi

    1. cellular response to osmotic stress Source: PomBase
    2. glutathione metabolic process Source: PomBase
    3. methylglyoxal catabolic process to D-lactate Source: PomBase

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Cobalt, Manganese, Metal-binding, Nickel, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00619; UER00675.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactoylglutathione lyase (EC:4.4.1.5)
    Alternative name(s):
    Aldoketomutase
    Glyoxalase I
    Short name:
    Glx I
    Ketone-aldehyde mutase
    Methylglyoxalase
    S-D-lactoylglutathione methylglyoxal lyase
    Gene namesi
    Name:glo1
    ORF Names:SPBC12C2.12c, SPBC21D10.03c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC12C2.12c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302Lactoylglutathione lyasePRO_0000168086Add
    BLAST

    Proteomic databases

    MaxQBiQ09751.
    PaxDbiQ09751.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi276288. 18 interactions.
    MINTiMINT-4694686.
    STRINGi4896.SPBC12C2.12c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09751.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glyoxalase I family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0346.
    HOGENOMiHOG000215632.
    KOiK01759.
    OMAiSTYRMNH.
    OrthoDBiEOG7HXD33.
    PhylomeDBiQ09751.

    Family and domain databases

    Gene3Di3.10.180.10. 2 hits.
    InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR004360. Glyas_Fos-R_dOase_dom.
    IPR004361. Glyoxalase_1.
    IPR018146. Glyoxalase_1_CS.
    [Graphical view]
    PfamiPF00903. Glyoxalase. 2 hits.
    [Graphical view]
    SUPFAMiSSF54593. SSF54593. 2 hits.
    TIGRFAMsiTIGR00068. glyox_I. 2 hits.
    PROSITEiPS00934. GLYOXALASE_I_1. 2 hits.
    PS00935. GLYOXALASE_I_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q09751-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTTDMSTY KLNHTMIRVK DLDKSLKFYT EVFGMKLIDQ WVFEENEFSL    50
    SFLAFDGPGA LNHGVERSKR EGILELTYNF GTEKKEGPVY INGNTEPKRG 100
    FGHICFTVDN IESACAYLES KGVSFKKKLS DGKMKHIAFA LDPDNYWIEL 150
    VSQSETKPKA NISNFRFNHT MVRVKDPEPS IAFYEKLGMK VIDKADHPNG 200
    KFTNYFLAYP SDLPRHDREG LLELTHNWGT EKESGPVYHN GNDGDEKGYG 250
    HVCISVDNIN AACSKFEAEG LPFKKKLTDG RMKDIAFLLD PDNYWVEVIE 300
    QK 302
    Length:302
    Mass (Da):34,410
    Last modified:November 1, 1995 - v1
    Checksum:iD5F916F51BA2A0F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA20759.1.
    PIRiT11675.
    RefSeqiNP_596010.1. NM_001021918.2.

    Genome annotation databases

    EnsemblFungiiSPBC12C2.12c.1; SPBC12C2.12c.1:pep; SPBC12C2.12c.
    GeneIDi2539736.
    KEGGispo:SPBC12C2.12c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA20759.1 .
    PIRi T11675.
    RefSeqi NP_596010.1. NM_001021918.2.

    3D structure databases

    ProteinModelPortali Q09751.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276288. 18 interactions.
    MINTi MINT-4694686.
    STRINGi 4896.SPBC12C2.12c-1.

    Proteomic databases

    MaxQBi Q09751.
    PaxDbi Q09751.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC12C2.12c.1 ; SPBC12C2.12c.1:pep ; SPBC12C2.12c .
    GeneIDi 2539736.
    KEGGi spo:SPBC12C2.12c.

    Organism-specific databases

    PomBasei SPBC12C2.12c.

    Phylogenomic databases

    eggNOGi COG0346.
    HOGENOMi HOG000215632.
    KOi K01759.
    OMAi STYRMNH.
    OrthoDBi EOG7HXD33.
    PhylomeDBi Q09751.

    Enzyme and pathway databases

    UniPathwayi UPA00619 ; UER00675 .

    Miscellaneous databases

    NextBioi 20800887.
    PROi Q09751.

    Family and domain databases

    Gene3Di 3.10.180.10. 2 hits.
    InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR004360. Glyas_Fos-R_dOase_dom.
    IPR004361. Glyoxalase_1.
    IPR018146. Glyoxalase_1_CS.
    [Graphical view ]
    Pfami PF00903. Glyoxalase. 2 hits.
    [Graphical view ]
    SUPFAMi SSF54593. SSF54593. 2 hits.
    TIGRFAMsi TIGR00068. glyox_I. 2 hits.
    PROSITEi PS00934. GLYOXALASE_I_1. 2 hits.
    PS00935. GLYOXALASE_I_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Identification of thermostable glyoxalase I in the fission yeast Schizosaccharomyces pombe."
      Takatsume Y., Izawa S., Inoue Y.
      Arch. Microbiol. 181:371-377(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiLGUL_SCHPO
    AccessioniPrimary (citable) accession number: Q09751
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3