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Protein

Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

SPBC12C2.11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathway: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing] (SPBC12C2.11)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_306868. Synthesis of UDP-N-acetyl-glucosamine.
UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
ORF Names:SPBC12C2.11, SPBC21D10.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC12C2.11.
PomBaseiSPBC12C2.11.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 696695Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135288Add
BLAST

Proteomic databases

MaxQBiQ09740.
PaxDbiQ09740.

Interactioni

Protein-protein interaction databases

MINTiMINT-4694590.
STRINGi4896.SPBC12C2.11.1.

Structurei

3D structure databases

ProteinModelPortaliQ09740.
SMRiQ09740. Positions 337-684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 303302Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini375 – 514140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini547 – 686140SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
InParanoidiQ09740.
KOiK00820.
OMAiMMVVLQI.
OrthoDBiEOG7034RG.
PhylomeDBiQ09740.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYINY LVERDRGYIL KTLVKGLKRL EYRGYDSSGC AVDGDEGEDF
60 70 80 90 100
IMFKEVGNVS KLEASIKGSN VNKSTKFINH CAISHTRWAT HGIPSPINCH
110 120 130 140 150
PQRSDPHSEF IVVHNGILTN YRELRTVLES RGMVFESETD TECVAKLAKF
160 170 180 190 200
IYDTTPGVDF TSLAKLVFRE LEGAYALLIK SSHYPGEVVA TRRGSPLIVG
210 220 230 240 250
VKSEQKLKVD FVDVEFPEPA EGLPGTPKPT SLHPVFSNPA TNGMLRGDKP
260 270 280 290 300
DLLHRAQSRA FVSGEGVPGP IEYFFASDAT PIIEYTKRVM FLEDDDIAHV
310 320 330 340 350
RDGELHVHRL RREGGGSTTR TIETLEMEIA SVMKGNYDHY MIKEICEQPD
360 370 380 390 400
SLLNTMRGRV NFVNRLVTLG GLESYYDIIR KSRRLIFVAC GTSYHSCVAV
410 420 430 440 450
RPLFEELTNI PVVVELASDF VDRCPSVFRD DTFIFVSQSG ETADSLLALQ
460 470 480 490 500
YTLENGALAI GVVNCVGSSI SRKTHCGVHI NAGPEICVAS TKAYTSQYVA
510 520 530 540 550
LVLMALYLSR DSVSRLERRN EIIDGLAEIG EKVQETLHLN AAIKQTAIEQ
560 570 580 590 600
LINKDKMLII GRGYHYATAL EGALKVKEIS YTHAEGVLAG ELKHGVLALV
610 620 630 640 650
DNDMPIVMLL PDDYNFPKAW NAFEQVRARG GKPIIITDKK LDNLEGFTII
660 670 680 690
KVPKTVDCLQ GILNVIPFQL LSYWLAVKRG HNVDQPRNLA KSVTVE
Length:696
Mass (Da):77,322
Last modified:January 23, 2007 - v2
Checksum:i9C4867168FB3EBFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA20758.1.
PIRiT11674.
RefSeqiNP_596011.1. NM_001021919.2.

Genome annotation databases

EnsemblFungiiSPBC12C2.11.1; SPBC12C2.11.1:pep; SPBC12C2.11.
GeneIDi2539622.
KEGGispo:SPBC12C2.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA20758.1.
PIRiT11674.
RefSeqiNP_596011.1. NM_001021919.2.

3D structure databases

ProteinModelPortaliQ09740.
SMRiQ09740. Positions 337-684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4694590.
STRINGi4896.SPBC12C2.11.1.

Proteomic databases

MaxQBiQ09740.
PaxDbiQ09740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC12C2.11.1; SPBC12C2.11.1:pep; SPBC12C2.11.
GeneIDi2539622.
KEGGispo:SPBC12C2.11.

Organism-specific databases

EuPathDBiFungiDB:SPBC12C2.11.
PomBaseiSPBC12C2.11.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
InParanoidiQ09740.
KOiK00820.
OMAiMMVVLQI.
OrthoDBiEOG7034RG.
PhylomeDBiQ09740.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.
ReactomeiREACT_306868. Synthesis of UDP-N-acetyl-glucosamine.

Miscellaneous databases

NextBioi20800778.
PROiQ09740.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiGFA1_SCHPO
AccessioniPrimary (citable) accession number: Q09740
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.