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Q09740

- GFA1_SCHPO

UniProt

Q09740 - GFA1_SCHPO

Protein

Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

SPBC12C2.11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: PomBase

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. chitin biosynthetic process Source: PomBase
    3. external encapsulating structure organization Source: PomBase
    4. glutamine metabolic process Source: UniProtKB-KW
    5. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_188301. XBP1(S) activates chaperone genes.
    REACT_188592. Synthesis of UDP-N-acetyl-glucosamine.
    UniPathwayiUPA00113; UER00528.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Short name:
    GFAT
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase
    Hexosephosphate aminotransferase
    Gene namesi
    ORF Names:SPBC12C2.11, SPBC21D10.02
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC12C2.11.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 696695Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135288Add
    BLAST

    Proteomic databases

    MaxQBiQ09740.
    PaxDbiQ09740.

    Interactioni

    Protein-protein interaction databases

    BioGridi276179. 2 interactions.
    MINTiMINT-4694590.
    STRINGi4896.SPBC12C2.11-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09740.
    SMRiQ09740. Positions 337-684.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 303302Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini375 – 514140SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini547 – 686140SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    HOGENOMiHOG000258898.
    KOiK00820.
    OMAiCRIPCEV.
    OrthoDBiEOG7034RG.
    PhylomeDBiQ09740.

    Family and domain databases

    Gene3Di3.60.20.10. 2 hits.
    InterProiIPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 2 hits.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q09740-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIFGYINY LVERDRGYIL KTLVKGLKRL EYRGYDSSGC AVDGDEGEDF    50
    IMFKEVGNVS KLEASIKGSN VNKSTKFINH CAISHTRWAT HGIPSPINCH 100
    PQRSDPHSEF IVVHNGILTN YRELRTVLES RGMVFESETD TECVAKLAKF 150
    IYDTTPGVDF TSLAKLVFRE LEGAYALLIK SSHYPGEVVA TRRGSPLIVG 200
    VKSEQKLKVD FVDVEFPEPA EGLPGTPKPT SLHPVFSNPA TNGMLRGDKP 250
    DLLHRAQSRA FVSGEGVPGP IEYFFASDAT PIIEYTKRVM FLEDDDIAHV 300
    RDGELHVHRL RREGGGSTTR TIETLEMEIA SVMKGNYDHY MIKEICEQPD 350
    SLLNTMRGRV NFVNRLVTLG GLESYYDIIR KSRRLIFVAC GTSYHSCVAV 400
    RPLFEELTNI PVVVELASDF VDRCPSVFRD DTFIFVSQSG ETADSLLALQ 450
    YTLENGALAI GVVNCVGSSI SRKTHCGVHI NAGPEICVAS TKAYTSQYVA 500
    LVLMALYLSR DSVSRLERRN EIIDGLAEIG EKVQETLHLN AAIKQTAIEQ 550
    LINKDKMLII GRGYHYATAL EGALKVKEIS YTHAEGVLAG ELKHGVLALV 600
    DNDMPIVMLL PDDYNFPKAW NAFEQVRARG GKPIIITDKK LDNLEGFTII 650
    KVPKTVDCLQ GILNVIPFQL LSYWLAVKRG HNVDQPRNLA KSVTVE 696
    Length:696
    Mass (Da):77,322
    Last modified:January 23, 2007 - v2
    Checksum:i9C4867168FB3EBFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA20758.1.
    PIRiT11674.
    RefSeqiNP_596011.1. NM_001021919.2.

    Genome annotation databases

    EnsemblFungiiSPBC12C2.11.1; SPBC12C2.11.1:pep; SPBC12C2.11.
    GeneIDi2539622.
    KEGGispo:SPBC12C2.11.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA20758.1 .
    PIRi T11674.
    RefSeqi NP_596011.1. NM_001021919.2.

    3D structure databases

    ProteinModelPortali Q09740.
    SMRi Q09740. Positions 337-684.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276179. 2 interactions.
    MINTi MINT-4694590.
    STRINGi 4896.SPBC12C2.11-1.

    Proteomic databases

    MaxQBi Q09740.
    PaxDbi Q09740.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC12C2.11.1 ; SPBC12C2.11.1:pep ; SPBC12C2.11 .
    GeneIDi 2539622.
    KEGGi spo:SPBC12C2.11.

    Organism-specific databases

    PomBasei SPBC12C2.11.

    Phylogenomic databases

    eggNOGi COG0449.
    HOGENOMi HOG000258898.
    KOi K00820.
    OMAi CRIPCEV.
    OrthoDBi EOG7034RG.
    PhylomeDBi Q09740.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00528 .
    Reactomei REACT_188301. XBP1(S) activates chaperone genes.
    REACT_188592. Synthesis of UDP-N-acetyl-glucosamine.

    Miscellaneous databases

    NextBioi 20800778.
    PROi Q09740.

    Family and domain databases

    Gene3Di 3.60.20.10. 2 hits.
    InterProi IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 2 hits.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiGFA1_SCHPO
    AccessioniPrimary (citable) accession number: Q09740
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3