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Protein

Probable ubiquitin carboxyl-terminal hydrolase 8

Gene

ubp8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541NucleophilePROSITE-ProRule annotation
Active sitei395 – 3951Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri36 – 9661UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: PomBase
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. chromatin remodeling Source: PomBase
  2. histone acetylation Source: PomBase
  3. histone deubiquitination Source: GO_Central
  4. histone H2B conserved C-terminal lysine deubiquitination Source: PomBase
  5. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  6. protein deubiquitination Source: PomBase
  7. regulation of proteasomal protein catabolic process Source: GO_Central
  8. regulation of transcription from RNA polymerase II promoter Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.A61.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase 8 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 8
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
Gene namesi
Name:ubp8
ORF Names:SPAC13A11.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13A11.04c.
PomBaseiSPAC13A11.04c.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: PomBase
  2. nucleus Source: PomBase
  3. SAGA complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Probable ubiquitin carboxyl-terminal hydrolase 8PRO_0000080609Add
BLAST

Proteomic databases

MaxQBiQ09738.

Interactioni

Protein-protein interaction databases

BioGridi278611. 109 interactions.
IntActiQ09738. 2 interactions.
MINTiMINT-8210504.
STRINGi4896.SPAC13A11.04c-1.

Structurei

3D structure databases

ProteinModelPortaliQ09738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 435291USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri36 – 9661UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5077.
InParanoidiQ09738.
KOiK11366.
OMAiSCALDEM.
OrthoDBiEOG7R2BSX.
PhylomeDBiQ09738.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGDVEGCQH LKLKPADVEN YQKICTQIFS CHFVPRRCST CKRINKRSIR
60 70 80 90 100
CLSCHSVGCL WGHHGEEHAM EHTHMIGVDV KNGHTYCFGC QDYVYQTELE
110 120 130 140 150
TLRFKIKNIK AWQSDHKRLP EKYNQMVCLE AYRKYPPVCA TAGLRGIQNL
160 170 180 190 200
GATCFMSVIL QSILHNPLVR NLFFSGFHTS TDCKRPTCMT CAIDDMFSSI
210 220 230 240 250
YNSKNKSTFY GPTAVLNLMW KLSKSLCGYS QQDGHEFFVY LLDQMHTESG
260 270 280 290 300
GGTSMPCTCP IHRIFSGSLK NVVTCLDCKK ERVAVDPLMD ISLDINEPTL
310 320 330 340 350
QGCLERFVSK EKVQYSCHSC GSKNAIKQLV FDKLPPTICM QLKRFEQNNF
360 370 380 390 400
AMSTKIDKQV SYPAFLRMRY NFNQDDVDYQ LYSVVCHKGT LDTGHYIAYT
410 420 430 440
YYQNQWFLLD DTTIVEVKES EVLNSQAYLL FYHERQILYS DEMTVKTEN
Length:449
Mass (Da):51,720
Last modified:November 1, 1995 - v1
Checksum:i281D265D064CB3FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90805.1.
PIRiT37611.
RefSeqiNP_592992.1. NM_001018391.2.

Genome annotation databases

EnsemblFungiiSPAC13A11.04c.1; SPAC13A11.04c.1:pep; SPAC13A11.04c.
GeneIDi2542135.
KEGGispo:SPAC13A11.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90805.1.
PIRiT37611.
RefSeqiNP_592992.1. NM_001018391.2.

3D structure databases

ProteinModelPortaliQ09738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278611. 109 interactions.
IntActiQ09738. 2 interactions.
MINTiMINT-8210504.
STRINGi4896.SPAC13A11.04c-1.

Protein family/group databases

MEROPSiC19.A61.

Proteomic databases

MaxQBiQ09738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC13A11.04c.1; SPAC13A11.04c.1:pep; SPAC13A11.04c.
GeneIDi2542135.
KEGGispo:SPAC13A11.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC13A11.04c.
PomBaseiSPAC13A11.04c.

Phylogenomic databases

eggNOGiCOG5077.
InParanoidiQ09738.
KOiK11366.
OMAiSCALDEM.
OrthoDBiEOG7R2BSX.
PhylomeDBiQ09738.

Miscellaneous databases

NextBioi20803207.
PROiQ09738.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiUBP8_SCHPO
AccessioniPrimary (citable) accession number: Q09738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.