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Protein

Probable ubiquitin carboxyl-terminal hydrolase 8

Gene

ubp8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154NucleophilePROSITE-ProRule annotation1
Active sitei395Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri36 – 96UBP-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: PomBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin remodeling Source: PomBase
  • histone acetylation Source: PomBase
  • histone H2B conserved C-terminal lysine deubiquitination Source: PomBase
  • protein deubiquitination Source: PomBase
  • regulation of transcription from RNA polymerase II promoter Source: PomBase
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.A61.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase 8 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 8
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
Gene namesi
Name:ubp8
ORF Names:SPAC13A11.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13A11.04c.
PomBaseiSPAC13A11.04c. ubp8.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: PomBase
  • nucleus Source: PomBase
  • SAGA complex Source: PomBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806091 – 449Probable ubiquitin carboxyl-terminal hydrolase 8Add BLAST449

Proteomic databases

MaxQBiQ09738.
PRIDEiQ09738.

Interactioni

Protein-protein interaction databases

BioGridi278611. 109 interactors.
IntActiQ09738. 2 interactors.
MINTiMINT-8210504.

Structurei

3D structure databases

ProteinModelPortaliQ09738.
SMRiQ09738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 435USPAdd BLAST291

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri36 – 96UBP-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiQ09738.
KOiK11366.
OMAiCEHIQRL.
OrthoDBiEOG092701A2.
PhylomeDBiQ09738.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGDVEGCQH LKLKPADVEN YQKICTQIFS CHFVPRRCST CKRINKRSIR
60 70 80 90 100
CLSCHSVGCL WGHHGEEHAM EHTHMIGVDV KNGHTYCFGC QDYVYQTELE
110 120 130 140 150
TLRFKIKNIK AWQSDHKRLP EKYNQMVCLE AYRKYPPVCA TAGLRGIQNL
160 170 180 190 200
GATCFMSVIL QSILHNPLVR NLFFSGFHTS TDCKRPTCMT CAIDDMFSSI
210 220 230 240 250
YNSKNKSTFY GPTAVLNLMW KLSKSLCGYS QQDGHEFFVY LLDQMHTESG
260 270 280 290 300
GGTSMPCTCP IHRIFSGSLK NVVTCLDCKK ERVAVDPLMD ISLDINEPTL
310 320 330 340 350
QGCLERFVSK EKVQYSCHSC GSKNAIKQLV FDKLPPTICM QLKRFEQNNF
360 370 380 390 400
AMSTKIDKQV SYPAFLRMRY NFNQDDVDYQ LYSVVCHKGT LDTGHYIAYT
410 420 430 440
YYQNQWFLLD DTTIVEVKES EVLNSQAYLL FYHERQILYS DEMTVKTEN
Length:449
Mass (Da):51,720
Last modified:November 1, 1995 - v1
Checksum:i281D265D064CB3FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90805.1.
PIRiT37611.
RefSeqiNP_592992.1. NM_001018391.2.

Genome annotation databases

EnsemblFungiiSPAC13A11.04c.1; SPAC13A11.04c.1:pep; SPAC13A11.04c.
GeneIDi2542135.
KEGGispo:SPAC13A11.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90805.1.
PIRiT37611.
RefSeqiNP_592992.1. NM_001018391.2.

3D structure databases

ProteinModelPortaliQ09738.
SMRiQ09738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278611. 109 interactors.
IntActiQ09738. 2 interactors.
MINTiMINT-8210504.

Protein family/group databases

MEROPSiC19.A61.

Proteomic databases

MaxQBiQ09738.
PRIDEiQ09738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC13A11.04c.1; SPAC13A11.04c.1:pep; SPAC13A11.04c.
GeneIDi2542135.
KEGGispo:SPAC13A11.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC13A11.04c.
PomBaseiSPAC13A11.04c. ubp8.

Phylogenomic databases

InParanoidiQ09738.
KOiK11366.
OMAiCEHIQRL.
OrthoDBiEOG092701A2.
PhylomeDBiQ09738.

Enzyme and pathway databases

ReactomeiR-SPO-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiQ09738.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBP8_SCHPO
AccessioniPrimary (citable) accession number: Q09738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 15, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.