Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lanosterol 14-alpha demethylase erg11

Gene

erg11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By similarity).By similarity

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase erg11 (erg11)
  2. Delta(14)-sterol reductase (erg24)
  3. Methylsterol monooxygenase (erg25)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (erg26)
  5. 3-keto-steroid reductase (erg27)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • ergosterol biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism, Stress response

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-SPO-191273. Cholesterol biosynthesis.
R-SPO-211976. Endogenous sterols.
R-SPO-2426168. Activation of gene expression by SREBF (SREBP).
UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase erg11 (EC:1.14.13.70)
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-14DM
Cytochrome P450-LIA1
Sterol 14-alpha demethylase erg11
Gene namesi
Name:erg11
Synonyms:cyp51
ORF Names:SPAC13A11.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13A11.02c.
PomBaseiSPAC13A11.02c. erg11.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Lanosterol 14-alpha demethylase erg11PRO_0000052009Add
BLAST

Proteomic databases

MaxQBiQ09736.

Expressioni

Inductioni

Up-regulated by sre1 in response to absence of oxygen.1 Publication

Interactioni

Subunit structurei

Interacts with dap1.1 Publication

Protein-protein interaction databases

BioGridi279053. 3 interactions.
MINTiMINT-4694544.

Structurei

3D structure databases

ProteinModelPortaliQ09736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000042780.
InParanoidiQ09736.
KOiK05917.
OMAiSTSIWAG.
OrthoDBiEOG092C1MV8.
PhylomeDBiQ09736.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSLVSILL SIALAWYVGY IINQLTSRNS KRPPIVFHWI PFVGSAVAYG
60 70 80 90 100
MDPYVFFREC RAKYGDVFTF VCMGRKMTAF LGVQGNDFLF NGKLADLNAE
110 120 130 140 150
EAYSHLTTPV FGKDVVYDIP NHVFMEHKKF IKSGLGFSQF RSYVPLILNE
160 170 180 190 200
MDAFLSTSPD FGPGKEGVAD LLKTMPVMTI YTASRTLQGA EVRKGFDAGF
210 220 230 240 250
ADLYHDLDQG FSPVNFVFPW LPLPRNRRRD RAHKIMQKTY LKIIKDRRSS
260 270 280 290 300
TENPGTDMIW TLMSCKYRDG RPLKEHEIAG MMIALLMAGQ HTSAATIVWV
310 320 330 340 350
LALLGSKPEI IEMLWEEQKR VVGENLELKF DQYKDMPLLN YVIQETLRLH
360 370 380 390 400
PPIHSHMRKV KRDLPVPGSK IVIPANNYLL AAPGLTATEE EYFTHATDFD
410 420 430 440 450
PKRWNDRVNE DENAEQIDYG YGLVTKGAAS PYLPFGAGRH RCIGEQFAYM
460 470 480 490
HLSTIISKFV HDYTWTLIGK VPNVDYSSMV ALPLGPVKIA WKRRN
Length:495
Mass (Da):56,331
Last modified:November 1, 1995 - v1
Checksum:i193333395F6C8B31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90803.1.
PIRiT37609.
RefSeqiNP_592990.1. NM_001018389.2.

Genome annotation databases

EnsemblFungiiSPAC13A11.02c.1; SPAC13A11.02c.1:pep; SPAC13A11.02c.
GeneIDi2542599.
KEGGispo:SPAC13A11.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90803.1.
PIRiT37609.
RefSeqiNP_592990.1. NM_001018389.2.

3D structure databases

ProteinModelPortaliQ09736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279053. 3 interactions.
MINTiMINT-4694544.

Proteomic databases

MaxQBiQ09736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC13A11.02c.1; SPAC13A11.02c.1:pep; SPAC13A11.02c.
GeneIDi2542599.
KEGGispo:SPAC13A11.02c.

Organism-specific databases

EuPathDBiFungiDB:SPAC13A11.02c.
PomBaseiSPAC13A11.02c. erg11.

Phylogenomic databases

HOGENOMiHOG000042780.
InParanoidiQ09736.
KOiK05917.
OMAiSTSIWAG.
OrthoDBiEOG092C1MV8.
PhylomeDBiQ09736.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.
ReactomeiR-SPO-191273. Cholesterol biosynthesis.
R-SPO-211976. Endogenous sterols.
R-SPO-2426168. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

PROiQ09736.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP51_SCHPO
AccessioniPrimary (citable) accession number: Q09736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.