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Protein

Metal-binding regulatory protein cuf1

Gene

cuf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Copper-sensing transcription factor that regulates iron uptake genes. Under copper starvation conditions activates the transcription of the copper transport genes, ctr4, ctr5 and ctr6.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11ZincPROSITE-ProRule annotation1
Metal bindingi14ZincPROSITE-ProRule annotation1
Metal bindingi23ZincPROSITE-ProRule annotation1
Metal bindingi25ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 40Copper-fistPROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • copper ion binding Source: PomBase
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: PomBase
  • RNA polymerase II transcription factor activity, copper ion regulated core promoter proximal region sequence-specific binding Source: PomBase

GO - Biological processi

  • cellular copper ion homeostasis Source: PomBase
  • cellular iron ion homeostasis Source: PomBase
  • cellular response to acidic pH Source: GO_Central
  • negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  • positive regulation of transcription from RNA polymerase II promoter Source: PomBase
  • regulation of transcription from RNA polymerase II promoter in response to copper ion starvation Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandCopper, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metal-binding regulatory protein cuf1
Gene namesi
Name:cuf1
ORF Names:SPAC31A2.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31A2.11c.
PomBaseiSPAC31A2.11c. cuf1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Cytoplasmic in presence of excess copper ions. The Cys-His motif (328-342) interacts with the N-terminal nuclear localization signal region leading to sequestration in the cytoplasm. Nuclear under copper starvation conditions.

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • nucleus Source: PomBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi13K → A: No nuclear localization. 1 Publication1
Mutagenesisi16R → A: No nuclear localization. 1 Publication1
Mutagenesisi19R → A: No nuclear localization. 1 Publication1
Mutagenesisi24K → A: No nuclear localization. 1 Publication1
Mutagenesisi28R → A: No nuclear localization. 1 Publication1
Mutagenesisi45K → A: No nuclear localization. 1 Publication1
Mutagenesisi47R → A: No nuclear localization. 1 Publication1
Mutagenesisi50R → A: No nuclear localization. 1 Publication1
Mutagenesisi53R → A: No nuclear localization. 1 Publication1
Mutagenesisi328C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi330C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi334C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi336C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi339C → A: Constitutive nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949301 – 411Metal-binding regulatory protein cuf1Add BLAST411

Proteomic databases

MaxQBiQ09728.
PRIDEiQ09728.

Interactioni

Protein-protein interaction databases

BioGridi279627. 8 interactors.
MINTiMINT-4694478.

Structurei

3D structure databases

ProteinModelPortaliQ09728.
SMRiQ09728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiQ09728.
OMAiKCRIARI.
OrthoDBiEOG092C4HSL.

Family and domain databases

Gene3Di3.90.430.10. 1 hit.
InterProiView protein in InterPro
IPR001083. Cu_fist_DNA-bd_dom.
PfamiView protein in Pfam
PF00649. Copper-fist. 1 hit.
PRINTSiPR00617. COPPERFIST.
SMARTiView protein in SMART
SM01090. Copper-fist. 1 hit.
SM00412. Cu_FIST. 1 hit.
SUPFAMiSSF57879. SSF57879. 1 hit.
PROSITEiView protein in PROSITE
PS01119. COPPER_FIST_1. 1 hit.
PS50073. COPPER_FIST_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVINNVKMA CMKCIRGHRS STCKHNDREL FPIRPKGRPI SQCEKCRIAR
60 70 80 90 100
ITRHLHVKCT CNSRKKGSKC STSSTTDLDS SSASNSSCSI PSSISEKLLP
110 120 130 140 150
RDNVKTHCPK RSASCCGKKP DVMPLKINLE SQTDFMGMPL QSQRPHSESY
160 170 180 190 200
RMLPEPEKFK SEYGYPSQFL PIEKLTSNVA YPPNYNNYLK SPYQQPTNFP
210 220 230 240 250
PEIQYNYSHS PQHSIQEAEE AAVYGPPVYR SGYQILYNNN TDSIAAAAAT
260 270 280 290 300
HDLYPQPDVP LTFAMLADGN YVPLPSSTNT YGPSNSYGYE ININESTNHV
310 320 330 340 350
DSSYLPHPIQ LSNYFTLPSS CAQADAACQC GDNCECLGCL THPNNATTLA
360 370 380 390 400
ALNHISALEK ETISHTDLHH TFKHEVNSSN NYELTNDELA ASSPLYTSSS
410
VPPSHITTGS T
Length:411
Mass (Da):45,473
Last modified:November 1, 1995 - v1
Checksum:iDB18E877B65D5699
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175404 Genomic DNA. Translation: AAD51063.1.
AJ243832 Genomic DNA. Translation: CAB52304.1.
CU329670 Genomic DNA. Translation: CAA90469.1.
PIRiT38609. S58105.
RefSeqiNP_592923.1. NM_001018324.2.

Genome annotation databases

EnsemblFungiiSPAC31A2.11c.1; SPAC31A2.11c.1:pep; SPAC31A2.11c.
GeneIDi2543198.
KEGGispo:SPAC31A2.11c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175404 Genomic DNA. Translation: AAD51063.1.
AJ243832 Genomic DNA. Translation: CAB52304.1.
CU329670 Genomic DNA. Translation: CAA90469.1.
PIRiT38609. S58105.
RefSeqiNP_592923.1. NM_001018324.2.

3D structure databases

ProteinModelPortaliQ09728.
SMRiQ09728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279627. 8 interactors.
MINTiMINT-4694478.

Proteomic databases

MaxQBiQ09728.
PRIDEiQ09728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC31A2.11c.1; SPAC31A2.11c.1:pep; SPAC31A2.11c.
GeneIDi2543198.
KEGGispo:SPAC31A2.11c.

Organism-specific databases

EuPathDBiFungiDB:SPAC31A2.11c.
PomBaseiSPAC31A2.11c. cuf1.

Phylogenomic databases

InParanoidiQ09728.
OMAiKCRIARI.
OrthoDBiEOG092C4HSL.

Miscellaneous databases

PROiPR:Q09728.

Family and domain databases

Gene3Di3.90.430.10. 1 hit.
InterProiView protein in InterPro
IPR001083. Cu_fist_DNA-bd_dom.
PfamiView protein in Pfam
PF00649. Copper-fist. 1 hit.
PRINTSiPR00617. COPPERFIST.
SMARTiView protein in SMART
SM01090. Copper-fist. 1 hit.
SM00412. Cu_FIST. 1 hit.
SUPFAMiSSF57879. SSF57879. 1 hit.
PROSITEiView protein in PROSITE
PS01119. COPPER_FIST_1. 1 hit.
PS50073. COPPER_FIST_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCUF1_SCHPO
AccessioniPrimary (citable) accession number: Q09728
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 15, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.