Q09728 (CUF1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metal-binding regulatory protein cuf1

Gene names

Name:	cuf1
ORF Names:	SPAC31A2.11c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Copper-sensing transcription factor that regulates iron uptake genes. Under copper starvation conditions activates the transcription of the copper transport genes, ctr4, ctr5 and ctr6. Ref.1 Ref.3
Subcellular location	Cytoplasm. Nucleus. Note: Cytoplasmic in presence of excess copper ions. The Cys-His motif (328-342) interacts with the N-terminal nuclear localization signal region leading to sequestration in the cytoplasm. Nuclear under copper starvation conditions. Ref.3
Sequence similarities	Contains 1 copper-fist DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	Copper DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular copper ion homeostasis Inferred from experiment PubMed 17384198. Source: PomBase cellular iron ion homeostasis Inferred from mutant phenotype Ref.1. Source: PomBase cellular response to cadmium ion Inferred from mutant phenotype PubMed 18684775. Source: PomBase detoxification of cadmium ion Inferred from mutant phenotype PubMed 18684775. Source: PomBase negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: PomBase positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: PomBase
Cellular_component	cytoplasm Inferred from direct assay Ref.3PubMed 17384198. Source: PomBase nucleus Inferred from direct assay Ref.3PubMed 16823372PubMed 17384198. Source: PomBase
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay Ref.1PubMed 12578838. Source: PomBase copper ion binding Inferred from mutant phenotype Ref.3. Source: PomBase copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay PubMed 12578838. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Metal-binding regulatory protein cuf1		PRO_0000194930
Regions
	DNA binding	1 – 40	40	Copper-fist
Sites
	Metal binding	11	1	Zinc By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	23	1	Zinc By similarity
	Metal binding	25	1	Zinc By similarity
Experimental info
	Mutagenesis	13	1	K → A: No nuclear localization. Ref.3
	Mutagenesis	16	1	R → A: No nuclear localization. Ref.3
	Mutagenesis	19	1	R → A: No nuclear localization. Ref.3
	Mutagenesis	24	1	K → A: No nuclear localization. Ref.3
	Mutagenesis	28	1	R → A: No nuclear localization. Ref.3
	Mutagenesis	45	1	K → A: No nuclear localization. Ref.3
	Mutagenesis	47	1	R → A: No nuclear localization. Ref.3
	Mutagenesis	50	1	R → A: No nuclear localization. Ref.3
	Mutagenesis	53	1	R → A: No nuclear localization. Ref.3
	Mutagenesis	328	1	C → A: Constitutive nuclear localization. Ref.3
	Mutagenesis	330	1	C → A: Constitutive nuclear localization. Ref.3
	Mutagenesis	334	1	C → A: Constitutive nuclear localization. Ref.3
	Mutagenesis	336	1	C → A: Constitutive nuclear localization. Ref.3
	Mutagenesis	339	1	C → A: Constitutive nuclear localization. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q09728 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: DB18E877B65D5699	FASTA	411	45,473
	10 20 30 40 50 60 MVVINNVKMA CMKCIRGHRS STCKHNDREL FPIRPKGRPI SQCEKCRIAR ITRHLHVKCT 70 80 90 100 110 120 CNSRKKGSKC STSSTTDLDS SSASNSSCSI PSSISEKLLP RDNVKTHCPK RSASCCGKKP 130 140 150 160 170 180 DVMPLKINLE SQTDFMGMPL QSQRPHSESY RMLPEPEKFK SEYGYPSQFL PIEKLTSNVA 190 200 210 220 230 240 YPPNYNNYLK SPYQQPTNFP PEIQYNYSHS PQHSIQEAEE AAVYGPPVYR SGYQILYNNN 250 260 270 280 290 300 TDSIAAAAAT HDLYPQPDVP LTFAMLADGN YVPLPSSTNT YGPSNSYGYE ININESTNHV 310 320 330 340 350 360 DSSYLPHPIQ LSNYFTLPSS CAQADAACQC GDNCECLGCL THPNNATTLA ALNHISALEK 370 380 390 400 410 ETISHTDLHH TFKHEVNSSN NYELTNDELA ASSPLYTSSS VPPSHITTGS T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A copper-sensing transcription factor regulates iron uptake genes in Schizosaccharomyces pombe." Labbe S., Pena M.M.O., Fernandes A.R., Thiele D.J. J. Biol. Chem. 274:36252-36260(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: FY254.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Copper induces cytoplasmic retention of fission yeast transcription factor cuf1." Beaudoin J., Labbe S. Eukaryot. Cell 5:277-292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-13; ARG-16; ARG-19; LYS-24; ARG-28; LYS-45; ARG-47; ARG-50; ARG-53; CYS-328; CYS-330; CYS-334; CYS-336 AND CYS-339.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175404 Genomic DNA. Translation: AAD51063.1. AJ243832 Genomic DNA. Translation: CAB52304.1. CU329670 Genomic DNA. Translation: CAA90469.1.
PIR	S58105. T38609.
RefSeq	NP_592923.1. NM_001018324.2.
3D structure databases
ProteinModelPortal	Q09728.
SMR	Q09728. Positions 1-41.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC31A2.11c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC31A2.11c.1; SPAC31A2.11c.1:pep; SPAC31A2.11c.
GeneID	2543198.
KEGG	spo:SPAC31A2.11c.
Organism-specific databases
PomBase	SPAC31A2.11c.
Phylogenomic databases
eggNOG	NOG71500.
OrthoDB	EOG4X0R35.
Family and domain databases
Gene3D	3.90.430.10. 1 hit.
InterPro	IPR001083. Cu_fist_DNA-bd. [Graphical view]
Pfam	PF00649. Copper-fist. 1 hit. [Graphical view]
PRINTS	PR00617. COPPERFIST.
SMART	SM00412. Cu_FIST. 1 hit. [Graphical view]
SUPFAM	SSF57879. Cu_fist_DNA_bd. 1 hit.
PROSITE	PS01119. COPPER_FIST_1. 1 hit. PS50073. COPPER_FIST_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804221.

Entry information

Entry name

CUF1_SCHPO

Accession

Primary (citable) accession number: Q09728

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program