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Protein

Metal-binding regulatory protein cuf1

Gene

cuf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Copper-sensing transcription factor that regulates iron uptake genes. Under copper starvation conditions activates the transcription of the copper transport genes, ctr4, ctr5 and ctr6.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11ZincPROSITE-ProRule annotation1
Metal bindingi14ZincPROSITE-ProRule annotation1
Metal bindingi23ZincPROSITE-ProRule annotation1
Metal bindingi25ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 40Copper-fistPROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • copper ion binding Source: PomBase
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: PomBase
  • RNA polymerase II transcription factor activity, copper ion regulated proximal promoter sequence-specific DNA binding Source: PomBase

GO - Biological processi

  • cellular copper ion homeostasis Source: PomBase
  • cellular iron ion homeostasis Source: PomBase
  • cellular response to acidic pH Source: GO_Central
  • negative regulation of transcription by RNA polymerase II Source: PomBase
  • positive regulation of transcription by RNA polymerase II Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandCopper, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metal-binding regulatory protein cuf1
Gene namesi
Name:cuf1
ORF Names:SPAC31A2.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31A2.11c
PomBaseiSPAC31A2.11c cuf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi13K → A: No nuclear localization. 1 Publication1
Mutagenesisi16R → A: No nuclear localization. 1 Publication1
Mutagenesisi19R → A: No nuclear localization. 1 Publication1
Mutagenesisi24K → A: No nuclear localization. 1 Publication1
Mutagenesisi28R → A: No nuclear localization. 1 Publication1
Mutagenesisi45K → A: No nuclear localization. 1 Publication1
Mutagenesisi47R → A: No nuclear localization. 1 Publication1
Mutagenesisi50R → A: No nuclear localization. 1 Publication1
Mutagenesisi53R → A: No nuclear localization. 1 Publication1
Mutagenesisi328C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi330C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi334C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi336C → A: Constitutive nuclear localization. 1 Publication1
Mutagenesisi339C → A: Constitutive nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949301 – 411Metal-binding regulatory protein cuf1Add BLAST411

Proteomic databases

MaxQBiQ09728
PaxDbiQ09728
PRIDEiQ09728

Interactioni

Protein-protein interaction databases

BioGridi279627, 8 interactors
STRINGi4896.SPAC31A2.11c.1

Structurei

3D structure databases

ProteinModelPortaliQ09728
SMRiQ09728
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiQ09728
OMAiKCRIARI
OrthoDBiEOG092C4HSL

Family and domain databases

Gene3Di3.90.430.10, 1 hit
InterProiView protein in InterPro
IPR001083 Cu_fist_DNA-bd_dom
IPR036395 Cu_fist_DNA-bd_dom_sf
PfamiView protein in Pfam
PF00649 Copper-fist, 1 hit
PRINTSiPR00617 COPPERFIST
SMARTiView protein in SMART
SM01090 Copper-fist, 1 hit
SM00412 Cu_FIST, 1 hit
SUPFAMiSSF57879 SSF57879, 1 hit
PROSITEiView protein in PROSITE
PS01119 COPPER_FIST_1, 1 hit
PS50073 COPPER_FIST_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q09728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVINNVKMA CMKCIRGHRS STCKHNDREL FPIRPKGRPI SQCEKCRIAR
60 70 80 90 100
ITRHLHVKCT CNSRKKGSKC STSSTTDLDS SSASNSSCSI PSSISEKLLP
110 120 130 140 150
RDNVKTHCPK RSASCCGKKP DVMPLKINLE SQTDFMGMPL QSQRPHSESY
160 170 180 190 200
RMLPEPEKFK SEYGYPSQFL PIEKLTSNVA YPPNYNNYLK SPYQQPTNFP
210 220 230 240 250
PEIQYNYSHS PQHSIQEAEE AAVYGPPVYR SGYQILYNNN TDSIAAAAAT
260 270 280 290 300
HDLYPQPDVP LTFAMLADGN YVPLPSSTNT YGPSNSYGYE ININESTNHV
310 320 330 340 350
DSSYLPHPIQ LSNYFTLPSS CAQADAACQC GDNCECLGCL THPNNATTLA
360 370 380 390 400
ALNHISALEK ETISHTDLHH TFKHEVNSSN NYELTNDELA ASSPLYTSSS
410
VPPSHITTGS T
Length:411
Mass (Da):45,473
Last modified:November 1, 1995 - v1
Checksum:iDB18E877B65D5699
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175404 Genomic DNA Translation: AAD51063.1
AJ243832 Genomic DNA Translation: CAB52304.1
CU329670 Genomic DNA Translation: CAA90469.1
PIRiT38609 S58105
RefSeqiNP_592923.1, NM_001018324.2

Genome annotation databases

EnsemblFungiiSPAC31A2.11c.1; SPAC31A2.11c.1:pep; SPAC31A2.11c
GeneIDi2543198
KEGGispo:SPAC31A2.11c

Entry informationi

Entry nameiCUF1_SCHPO
AccessioniPrimary (citable) accession number: Q09728
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health