Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent RNA helicase dbp10

Gene

dbp10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi113 – 1208ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase dbp10 (EC:3.6.4.13)
Gene namesi
Name:dbp10
ORF Names:SPAC31A2.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31A2.07c.
PomBaseiSPAC31A2.07c. dbp10.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848ATP-dependent RNA helicase dbp10PRO_0000055041Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei638 – 6381Phosphoserine1 Publication
Modified residuei733 – 7331Phosphoserine1 Publication
Modified residuei736 – 7361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09719.

PTM databases

iPTMnetiQ09719.

Interactioni

Protein-protein interaction databases

BioGridi279626. 3 interactions.
MINTiMINT-4694383.

Structurei

3D structure databases

ProteinModelPortaliQ09719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 272173Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini330 – 480151Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 9729Q motifAdd
BLAST
Motifi220 – 2234DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000246455.
InParanoidiQ09719.
KOiK14808.
OMAiFPPQPKV.
OrthoDBiEOG7FR7R5.
PhylomeDBiQ09719.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR012541. DBP10_C.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF08147. DBP10CT. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
ProDomiPD024971. DBP10CT. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01123. DBP10CT. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFQTSDEV DISNSLKAFP VDIATDNQKD KHENVGENVS DEDDGNYIAS
60 70 80 90 100
KLLESNRRTK GKKGNGKASN FQSMGLNQTL LRAIFKKGFK APTPIQRKTI
110 120 130 140 150
PLLLEGRDVV GMARTGSGKT AAFVIPMIEH LKSTLANSNT RALILSPNRE
160 170 180 190 200
LALQTVKVVK DFSKGTDLRS VAIVGGVSLE EQFSLLSGKP DIVVATPGRF
210 220 230 240 250
LHLKVEMKLE LSSIEYVVFD EADRLFEMGF AAQLTEILHA LPTSRQTLLF
260 270 280 290 300
SATLPRTLVD FAKAGLQDPV LVRLDVESKV SADLQSAFFS VKTAEREAAL
310 320 330 340 350
LCILQDIIKL PLKDNVRPRE IGNVNNPKKR KRALELALKG SESGSPDSTL
360 370 380 390 400
VFVPTKHHVE YVSELLVQAG YSVSKIYGSL DQEARLNEIN NFRLGKTNLL
410 420 430 440 450
VVTDVASRGI DIPLLANVIN YDFPPQPKVF VHRVGRTARA GRTGWAYSLV
460 470 480 490 500
RAEDAGYLLD LQLFLNRPLV TSSKQVKTDS DCDFTKQIVL GSLPQELVAE
510 520 530 540 550
LLEWVQRIVS RDVELQQLSN VAARGEKLYF RTRATCSAES AKRAKELVDS
560 570 580 590 600
KGWSSNNPLF GDVSVIEAEE KYAELLSKVS SYRPSETVFE IGQRGHLKTE
610 620 630 640 650
AAEIMRKRRN KVKPKGIKSE VASDKITDSS PGNMSEASES ELEEVFKNPK
660 670 680 690 700
ELSKKKTTDF KDKEYYMSHY APKESIQETG YAINSGENFT TAARHAILDL
710 720 730 740 750
TNDEGIEQSR KGGQRWDPKK KKFVNIINDE DGSKGSPKII RGESGVKLPA
760 770 780 790 800
TYRSGRFDEW KASKAFGAND SPIRENKRYK HNKLQTPKPA DKFRDNYHKQ
810 820 830 840
NKRNREAKER GIGIKVNSEL KSAVEIRKAR ELKEKRLAKN NRPSKKHR
Length:848
Mass (Da):94,661
Last modified:November 1, 1995 - v1
Checksum:i63E9870572BCA074
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90465.1.
AB027882 Genomic DNA. Translation: BAA87186.1.
PIRiS59645.
RefSeqiNP_592919.1. NM_001018320.2.

Genome annotation databases

EnsemblFungiiSPAC31A2.07c.1; SPAC31A2.07c.1:pep; SPAC31A2.07c.
GeneIDi2543197.
KEGGispo:SPAC31A2.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90465.1.
AB027882 Genomic DNA. Translation: BAA87186.1.
PIRiS59645.
RefSeqiNP_592919.1. NM_001018320.2.

3D structure databases

ProteinModelPortaliQ09719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279626. 3 interactions.
MINTiMINT-4694383.

PTM databases

iPTMnetiQ09719.

Proteomic databases

MaxQBiQ09719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC31A2.07c.1; SPAC31A2.07c.1:pep; SPAC31A2.07c.
GeneIDi2543197.
KEGGispo:SPAC31A2.07c.

Organism-specific databases

EuPathDBiFungiDB:SPAC31A2.07c.
PomBaseiSPAC31A2.07c. dbp10.

Phylogenomic databases

HOGENOMiHOG000246455.
InParanoidiQ09719.
KOiK14808.
OMAiFPPQPKV.
OrthoDBiEOG7FR7R5.
PhylomeDBiQ09719.

Miscellaneous databases

PROiQ09719.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR012541. DBP10_C.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF08147. DBP10CT. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
ProDomiPD024971. DBP10CT. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01123. DBP10CT. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 75-167, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638; SER-733 AND SER-736, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiDBP10_SCHPO
AccessioniPrimary (citable) accession number: Q09719
Secondary accession number(s): Q9USB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.