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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

wrs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:wrs1
ORF Names:SPAC2F7.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC2F7.13c.
PomBaseiSPAC2F7.13c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Tryptophan--tRNA ligase, cytoplasmicPRO_0000136743Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei288 – 2881Phosphothreonine1 Publication
Modified residuei290 – 2901Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09692.
PaxDbiQ09692.

Interactioni

Protein-protein interaction databases

MINTiMINT-4694127.
STRINGi4896.SPAC2F7.13c-1.

Structurei

3D structure databases

ProteinModelPortaliQ09692.
SMRiQ09692. Positions 26-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi91 – 10010"HIGH" region
Motifi275 – 2795"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224742.
InParanoidiQ09692.
KOiK01867.
OMAiARHKARS.
OrthoDBiEOG74N5SR.
PhylomeDBiQ09692.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEEQIVTP WDVKGSIVDG EEKGIDYERL IVQFGTRKIT PEQLERFEKL
60 70 80 90 100
TGKKPHLLLR RGAFFSHRDF DMILDRYEQK KPFYLYTGRG PSSDSMHLGH
110 120 130 140 150
MIPFMFCKWL QDVFQVPLVI QLTDDEKFLF KQGVSLEDCQ RFARENAKDI
160 170 180 190 200
IAVGFDPKKT FIFMNSTYVG GAFYQNVVRI AKCITANQSK ACFGFTDSDS
210 220 230 240 250
IGKIHFASIQ AAPSFSSSFP HIFNGAKDIP CLIPCAIDQD PYFRLTRDVS
260 270 280 290 300
GRLKFKKPAL LHSRFFPALQ GPQSKMSASK DSSAIFMTDT PNKIKNKINR
310 320 330 340 350
HAFSGGGATI EIHREKGGNP DVDVAYQYLS FFLDDDEKLK QLYNTYKAGT
360 370 380 390
LSTGEMKGEC IKLLQQFVSD FQAARSKVDE ATLDMFMDGS RKLEW
Length:395
Mass (Da):44,910
Last modified:November 1, 1995 - v1
Checksum:iE656AE8B76C5FDF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90500.1.
PIRiS58157.
RefSeqiNP_592983.1. NM_001018383.2.

Genome annotation databases

EnsemblFungiiSPAC2F7.13c.1; SPAC2F7.13c.1:pep; SPAC2F7.13c.
GeneIDi2541841.
KEGGispo:SPAC2F7.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA90500.1.
PIRiS58157.
RefSeqiNP_592983.1. NM_001018383.2.

3D structure databases

ProteinModelPortaliQ09692.
SMRiQ09692. Positions 26-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4694127.
STRINGi4896.SPAC2F7.13c-1.

Proteomic databases

MaxQBiQ09692.
PaxDbiQ09692.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC2F7.13c.1; SPAC2F7.13c.1:pep; SPAC2F7.13c.
GeneIDi2541841.
KEGGispo:SPAC2F7.13c.

Organism-specific databases

EuPathDBiFungiDB:SPAC2F7.13c.
PomBaseiSPAC2F7.13c.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224742.
InParanoidiQ09692.
KOiK01867.
OMAiARHKARS.
OrthoDBiEOG74N5SR.
PhylomeDBiQ09692.

Miscellaneous databases

NextBioi20802929.
PROiQ09692.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-288 AND THR-290, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSYWC_SCHPO
AccessioniPrimary (citable) accession number: Q09692
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.