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Protein

DNA repair protein rad32

Gene

rad32

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the repair of double strand breaks (DSB) caused by gamma and UV radiation. May work in conjunction with rhp51.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei134Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA damage response, detection of DNA damage Source: PomBase
  • DNA double-strand break processing Source: PomBase
  • double-strand break repair Source: PomBase
  • double-strand break repair involved in meiotic recombination Source: PomBase
  • double-strand break repair via homologous recombination Source: PomBase
  • double-strand break repair via nonhomologous end joining Source: PomBase
  • intra-S DNA damage checkpoint Source: PomBase
  • meiotic DNA double-strand break clipping Source: PomBase
  • meiotic DNA double-strand break formation Source: PomBase
  • reciprocal meiotic recombination Source: PomBase
  • signal transduction involved in DNA damage checkpoint Source: PomBase
  • telomere maintenance Source: PomBase

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, Meiosis
LigandManganese

Enzyme and pathway databases

ReactomeiR-SPO-1834949 Cytosolic sensors of pathogen-associated DNA
R-SPO-2559586 DNA Damage/Telomere Stress Induced Senescence
R-SPO-5693548 Sensing of DNA Double Strand Breaks
R-SPO-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rad32
Gene namesi
Name:rad32
ORF Names:SPAC13C5.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13C5.07
PomBaseiSPAC13C5.07

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001386831 – 649DNA repair protein rad32Add BLAST649

Proteomic databases

MaxQBiQ09683
PaxDbiQ09683
PRIDEiQ09683

Interactioni

Subunit structurei

Associates with nbn. Forms a multisubunit endonuclease complex, MRN, together with nbn and rad50.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
nbs1O430706EBI-2124866,EBI-2125045

Protein-protein interaction databases

BioGridi279207, 134 interactors
DIPiDIP-52388N
IntActiQ09683, 2 interactors
STRINGi4896.SPAC13C5.07.1

Structurei

Secondary structure

1649
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 23Combined sources6
Turni29 – 33Combined sources5
Turni35 – 39Combined sources5
Helixi40 – 54Combined sources15
Beta strandi58 – 62Combined sources5
Beta strandi67 – 71Combined sources5
Helixi74 – 88Combined sources15
Beta strandi89 – 91Combined sources3
Beta strandi97 – 100Combined sources4
Helixi115 – 117Combined sources3
Beta strandi123 – 125Combined sources3
Beta strandi127 – 129Combined sources3
Helixi133 – 135Combined sources3
Helixi145 – 151Combined sources7
Beta strandi154 – 157Combined sources4
Beta strandi167 – 169Combined sources3
Beta strandi172 – 176Combined sources5
Beta strandi179 – 186Combined sources8
Helixi191 – 199Combined sources9
Beta strandi203 – 209Combined sources7
Turni210 – 214Combined sources5
Beta strandi215 – 223Combined sources9
Beta strandi228 – 233Combined sources6
Helixi236 – 238Combined sources3
Beta strandi244 – 250Combined sources7
Beta strandi255 – 261Combined sources7
Turni262 – 265Combined sources4
Beta strandi266 – 270Combined sources5
Helixi281 – 284Combined sources4
Beta strandi288 – 295Combined sources8
Beta strandi298 – 305Combined sources8
Beta strandi307 – 309Combined sources3
Beta strandi312 – 318Combined sources7
Helixi319 – 321Combined sources3
Beta strandi327 – 329Combined sources3
Helixi332 – 358Combined sources27
Beta strandi373 – 379Combined sources7
Turni381 – 383Combined sources3
Helixi389 – 394Combined sources6
Turni395 – 399Combined sources5
Beta strandi406 – 410Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FBKX-ray2.38A/B15-413[»]
4FBQX-ray2.50A/B15-413[»]
4FBWX-ray2.20A/B7-413[»]
4FCXX-ray3.00A/B15-413[»]
ProteinModelPortaliQ09683
SMRiQ09683
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

HOGENOMiHOG000216581
InParanoidiQ09683
KOiK10865
OMAiIHFFRHR
OrthoDBiEOG092C2IFF
PhylomeDBiQ09683

Family and domain databases

Gene3Di3.30.110.110, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR003701 Mre11
IPR007281 Mre11_DNA-bd
IPR038487 Mre11_DNA-bd_sf
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF04152 Mre11_DNA_bind, 1 hit
PIRSFiPIRSF000882 DSB_repair_MRE11, 1 hit
SMARTiView protein in SMART
SM01347 Mre11_DNA_bind, 1 hit
TIGRFAMsiTIGR00583 mre11, 1 hit

Sequencei

Sequence statusi: Complete.

Q09683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNDPSDMNN ELHNENTIRI LISSDPHVGY GEKDPVRGND SFVSFNEILE
60 70 80 90 100
IARERDVDMI LLGGDIFHDN KPSRKALYQA LRSLRLNCLG DKPCELELLS
110 120 130 140 150
DTSLTTGDTA VCNINYLDPN INVAIPVFSI HGNHDDPSGD GRYSALDILQ
160 170 180 190 200
VTGLVNYFGR VPENDNIVVS PILLQKGFTK LALYGISNVR DERLYHSFRE
210 220 230 240 250
NKVKFLRPDL YRDEWFNLLT VHQNHSAHTP TSYLPESFIQ DFYDFVLWGH
260 270 280 290 300
EHECLIDGSY NPTQKFTVVQ PGSTIATSLS PGETAPKHCG ILNITGKDFH
310 320 330 340 350
LEKIRLRTVR PFIMKDIILS EVSSIPPMVE NKKEVLTYLI SKVEEAITEA
360 370 380 390 400
NAQWYEAQGT VPVVENEKPP LPLIRLRVDY TGGYQTENPQ RFSNRFVGRV
410 420 430 440 450
ANATDVVQFY LKKKYTRSKR NDGLYTSAVE DIKINSLRVE SLVNEYLKTN
460 470 480 490 500
RLECLPEDSL GEAVVNFVEK DDRDAIKECV ETQLNKQINL LVKKRVTEEN
510 520 530 540 550
LEQEISSIIN DLPKISTTKR KDYEELPEEV SETSINIAEH TPVLKHTSSL
560 570 580 590 600
LDHHSPLATS SSEHEMEATP SPALLKKTNK RRELPSSLTK KNTRTPQRSK
610 620 630 640
EVKKVPARKL SQSTKKSDKN TQSTLLFYDP SSTTEAQYLD NEDDEILDD
Length:649
Mass (Da):73,689
Last modified:November 1, 1995 - v1
Checksum:i400B349EF4FA3428
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82322 Genomic DNA Translation: CAA57765.1 Different termination.
CU329670 Genomic DNA Translation: CAA90458.1
PIRiS58097
RefSeqiNP_592935.1, NM_001018336.2

Genome annotation databases

EnsemblFungiiSPAC13C5.07.1; SPAC13C5.07.1:pep; SPAC13C5.07
GeneIDi2542757
KEGGispo:SPAC13C5.07

Similar proteinsi

Entry informationi

Entry nameiRAD32_SCHPO
AccessioniPrimary (citable) accession number: Q09683
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health