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Q09683 (RAD32_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair protein rad32
Gene names
Name:rad32
ORF Names:SPAC13C5.07
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length649 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the repair of double strand breaks (DSB) caused by gamma and UV radiation. May work in conjunction with rhp51.

Subunit structure

Associates with nbn. Forms a multisubunit endonuclease complex, MRN, together with nbn and rad50. Ref.3

Subcellular location

Nucleus Ref.3.

Sequence similarities

Belongs to the MRE11/RAD32 family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Meiosis
   Cellular componentNucleus
   LigandManganese
   Molecular functionEndonuclease
Exonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA double-strand break processing

Inferred from genetic interaction PubMed 17936710. Source: PomBase

cellular protein localization

Inferred from mutant phenotype PubMed 17936710. Source: PomBase

double-strand break repair via homologous recombination

Inferred from genetic interaction PubMed 12944481. Source: PomBase

double-strand break repair via nonhomologous end joining

Inferred from mutant phenotype PubMed 10373582PubMed 19211838. Source: PomBase

intra-S DNA damage checkpoint

Inferred from mutant phenotype Ref.3. Source: PomBase

meiotic DNA double-strand break formation

Traceable author statement PubMed 15654094. Source: PomBase

meiotic gene conversion

Inferred from direct assay PubMed 12628934. Source: PomBase

reciprocal meiotic recombination

Inferred from mutant phenotype Ref.1. Source: PomBase

telomere maintenance

Inferred from mutant phenotype PubMed 10373582. Source: PomBase

   Cellular_componentMre11 complex

Traceable author statement Ref.3. Source: PomBase

   Molecular_functiondouble-stranded DNA specific 3'-5' exodeoxyribonuclease activity

Traceable author statement PubMed 15485922. Source: PomBase

manganese ion binding

Inferred from electronic annotation. Source: InterPro

single-stranded DNA specific 3'-5' exodeoxyribonuclease activity

Traceable author statement PubMed 15485922. Source: PomBase

single-stranded DNA specific endodeoxyribonuclease activity

Traceable author statement PubMed 15485922. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 649649DNA repair protein rad32
PRO_0000138683

Sites

Active site1341Proton donor By similarity

Secondary structure

1 ........................................................................... 649
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q09683 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 400B349EF4FA3428

FASTA64973,689
        10         20         30         40         50         60 
MPNDPSDMNN ELHNENTIRI LISSDPHVGY GEKDPVRGND SFVSFNEILE IARERDVDMI 

        70         80         90        100        110        120 
LLGGDIFHDN KPSRKALYQA LRSLRLNCLG DKPCELELLS DTSLTTGDTA VCNINYLDPN 

       130        140        150        160        170        180 
INVAIPVFSI HGNHDDPSGD GRYSALDILQ VTGLVNYFGR VPENDNIVVS PILLQKGFTK 

       190        200        210        220        230        240 
LALYGISNVR DERLYHSFRE NKVKFLRPDL YRDEWFNLLT VHQNHSAHTP TSYLPESFIQ 

       250        260        270        280        290        300 
DFYDFVLWGH EHECLIDGSY NPTQKFTVVQ PGSTIATSLS PGETAPKHCG ILNITGKDFH 

       310        320        330        340        350        360 
LEKIRLRTVR PFIMKDIILS EVSSIPPMVE NKKEVLTYLI SKVEEAITEA NAQWYEAQGT 

       370        380        390        400        410        420 
VPVVENEKPP LPLIRLRVDY TGGYQTENPQ RFSNRFVGRV ANATDVVQFY LKKKYTRSKR 

       430        440        450        460        470        480 
NDGLYTSAVE DIKINSLRVE SLVNEYLKTN RLECLPEDSL GEAVVNFVEK DDRDAIKECV 

       490        500        510        520        530        540 
ETQLNKQINL LVKKRVTEEN LEQEISSIIN DLPKISTTKR KDYEELPEEV SETSINIAEH 

       550        560        570        580        590        600 
TPVLKHTSSL LDHHSPLATS SSEHEMEATP SPALLKKTNK RRELPSSLTK KNTRTPQRSK 

       610        620        630        640 
EVKKVPARKL SQSTKKSDKN TQSTLLFYDP SSTTEAQYLD NEDDEILDD

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterisation of the Schizosaccharomyces pombe rad32 gene: a gene required for repair of double strand breaks and recombination."
Tavassoli M., Shayeghi M., Nasim A., Watts F.Z.
Nucleic Acids Res. 23:383-388(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"The fission yeast Rad32 (Mre11)-Rad50-Nbs1 complex is required for the S-phase DNA damage checkpoint."
Chahwan C., Nakamura T.M., Sivakumar S., Russell P., Rhind N.
Mol. Cell. Biol. 23:6564-6573(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X82322 Genomic DNA. Translation: CAA57765.1. Different termination.
CU329670 Genomic DNA. Translation: CAA90458.1.
PIRS58097.
RefSeqNP_592935.1. NM_001018336.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4FBKX-ray2.38A/B15-413[»]
4FBQX-ray2.50A/B15-413[»]
4FBWX-ray2.20A/B7-413[»]
4FCXX-ray3.00A/B15-413[»]
ProteinModelPortalQ09683.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-52388N.
IntActQ09683. 2 interactions.
STRING4896.SPAC13C5.07-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC13C5.07.1; SPAC13C5.07.1:pep; SPAC13C5.07.
GeneID2542757.
KEGGspo:SPAC13C5.07.

Organism-specific databases

PomBaseSPAC13C5.07.

Phylogenomic databases

eggNOGCOG0420.
HOGENOMHOG000216581.
KOK10865.
OMAFVNKQVT.
OrthoDBEOG4P2T9Q.

Family and domain databases

InterProIPR003701. DNA_repair_Mre11.
IPR004843. Metallo_PEstase_dom.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERPTHR10139. PTHR10139. 1 hit.
PfamPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFPIRSF000882. DSB_repair_MRE11. 1 hit.
TIGRFAMsTIGR00583. mre11. 1 hit.
ProtoNetSearch...

Other

NextBio20803802.

Entry information

Entry nameRAD32_SCHPO
AccessionPrimary (citable) accession number: Q09683
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents