Q09682 (PSA4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable proteasome subunit alpha type-4
EC=3.4.25.1

Gene names

ORF Names:

SPAC13C5.01c, SPAC31A2.17c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe regulation of mitotic cell cycle Inferred by curator. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe proteasome core complex, alpha-subunit complex Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Probable proteasome subunit alpha type-4		PRO_0000124115

Sequences

	Sequence		Length	Mass (Da)
	Q09682 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 66C32773C5D10781	FASTA	248	27,925
	10 20 30 40 50 60 MSRSYDSRTT IFSPEGRLYQ VEYALEAINH AGVALGIVAK DGIVLAAEKK VTSKLLEQEE 70 80 90 100 110 120 SAEKLYHIGD NMLCAVAGLT ADANILINYA RRVGQQYLQT FNEEMPCEQL VRRVCDLKQG 130 140 150 160 170 180 YTQYGGLRPF GVSFLYAGWD HIRGYQLFQS NPSGNYGSWQ ANSIGGNSTS VQSLMRQEYK 190 200 210 220 230 240 DDINLDEASA MAVKFLSKTL DSNSLTHEKI EFATITKDTT KNKMVCKIWK SDEINEVLNK YQETQRQS

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA90475.1.
PIR	S58093.
RefSeq	XP_001713040.1. XM_001712988.1.
3D structure databases
ProteinModelPortal	Q09682.
SMR	Q09682. Positions 2-240.
ModBase	Search...
Protein-protein interaction databases
STRING	Q09682.
Protein family/group databases
MEROPS	T01.973.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC13C5.01c.1; SPAC13C5.01c.1:pep; SPAC13C5.01c.
GeneID	3361518.
KEGG	spo:SPAC13C5.01c.
Organism-specific databases
GeneDB_Spombe	SPAC13C5.01c.
Phylogenomic databases
eggNOG	fuNOG04658.
GeneTree	EFGT00050000002415.
HOGENOM	HBG499923.
OMA	MARRYDS.
OrthoDB	EOG47Q1G3.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001970-MONOMER.
Gene expression databases
ArrayExpress	Q09682.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR016050. Proteasome_bsu_CS. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K02728.
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA4_SCHPO

Accession

Primary (citable) accession number: Q09682

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program