Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione-independent glyoxalase hsp3102

Gene

hsp3102

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step (PubMed:24758716). May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.1 Publication

Kineticsi

kcat is 58.0 min(-1) with methylglyoxal as substrate.1 Publication
  1. KM=2.7 mM for methylglyoxal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei141By similarity1
    Active sitei142By similarity1
    Active sitei175By similarity1

    GO - Molecular functioni

    • glyoxalase III activity Source: PomBase

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    Biological processStress response

    Protein family/group databases

    MEROPSiC56.A05.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase hsp31021 Publication (EC:4.2.1.1301 Publication)
    Alternative name(s):
    Glyoxalase 3 homolog 21 Publication
    Heat shock protein 31 homolog 21 Publication
    Gene namesi
    Name:hsp31021 Publication
    ORF Names:SPAC5H10.02cImported
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC5H10.02c.
    PomBaseiSPAC5H10.02c. hsp3102.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: PomBase
    • cytosol Source: PomBase
    • nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001578541 – 240Glutathione-independent glyoxalase hsp3102Add BLAST240

    Proteomic databases

    MaxQBiQ09675.
    PRIDEiQ09675.

    Interactioni

    Protein-protein interaction databases

    BioGridi278290. 2 interactors.
    MINTiMINT-4693971.
    STRINGi4896.SPAC5H10.02c.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09675.
    SMRiQ09675.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000181653.
    InParanoidiQ09675.
    OMAiNEIFYED.
    OrthoDBiEOG092C4O4J.
    PhylomeDBiQ09675.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    PfamiView protein in Pfam
    PF01965. DJ-1_PfpI. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q09675-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSIAKGKNAL LVASSYYGPF YPDGKNTGVH FSELLIPYNV FKKAGFNVQF
    60 70 80 90 100
    VSENGSYKFD DHSIEESKLG DFERKVFNDK NDDFWTNLNN MKKASDIVGK
    110 120 130 140 150
    DYQLLFVAGG HAAMFDLPKA TNLQAVAREV FTNGGVLSAV CHGPVLLANV
    160 170 180 190 200
    KNPQSVEGKT VVYHKHVTAF NKAGEEKMGV MDELKKRGMK SLNEIFAEAG
    210 220 230 240
    ATFIDPPNPN VNFTQIDGKI VTGVNPQSAK STAEAAVSAL
    Length:240
    Mass (Da):26,117
    Last modified:November 1, 1995 - v1
    Checksum:i22C5614F06D56296
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA89952.1.
    PIRiS55480.
    RefSeqiNP_592815.1. NM_001018215.2.

    Genome annotation databases

    EnsemblFungiiSPAC5H10.02c.1; SPAC5H10.02c.1:pep; SPAC5H10.02c.
    GeneIDi2541799.
    KEGGispo:SPAC5H10.02c.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiHSP32_SCHPO
    AccessioniPrimary (citable) accession number: Q09675
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: July 5, 2017
    This is version 107 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families