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Protein

Glutathione-independent glyoxalase hsp3102

Gene

hsp3102

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step (PubMed:24758716). May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.1 Publication

Kineticsi

kcat is 58.0 min(-1) with methylglyoxal as substrate.1 Publication

  1. KM=2.7 mM for methylglyoxal1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei141 – 1411By similarity
    Active sitei142 – 1421By similarity
    Active sitei175 – 1751By similarity

    GO - Molecular functioni

    • glyoxalase III activity Source: PomBase

    GO - Biological processi

    • cellular detoxification Source: PomBase
    • lactate biosynthetic process Source: GOC
    • methylglyoxal catabolic process to lactate Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Stress response

    Protein family/group databases

    MEROPSiC56.A05.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase hsp31021 Publication (EC:4.2.1.1301 Publication)
    Alternative name(s):
    Glyoxalase 3 homolog 21 Publication
    Heat shock protein 31 homolog 21 Publication
    Gene namesi
    Name:hsp31021 Publication
    ORF Names:SPAC5H10.02cImported
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC5H10.02c.
    PomBaseiSPAC5H10.02c. hsp3102.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: PomBase
    • cytosol Source: PomBase
    • nucleus Source: PomBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240Glutathione-independent glyoxalase hsp3102PRO_0000157854Add
    BLAST

    Proteomic databases

    MaxQBiQ09675.

    Interactioni

    Protein-protein interaction databases

    BioGridi278290. 2 interactions.
    MINTiMINT-4693971.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09675.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000181653.
    InParanoidiQ09675.
    OMAiASEMGEN.
    OrthoDBiEOG73547H.
    PhylomeDBiQ09675.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q09675-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSIAKGKNAL LVASSYYGPF YPDGKNTGVH FSELLIPYNV FKKAGFNVQF
    60 70 80 90 100
    VSENGSYKFD DHSIEESKLG DFERKVFNDK NDDFWTNLNN MKKASDIVGK
    110 120 130 140 150
    DYQLLFVAGG HAAMFDLPKA TNLQAVAREV FTNGGVLSAV CHGPVLLANV
    160 170 180 190 200
    KNPQSVEGKT VVYHKHVTAF NKAGEEKMGV MDELKKRGMK SLNEIFAEAG
    210 220 230 240
    ATFIDPPNPN VNFTQIDGKI VTGVNPQSAK STAEAAVSAL
    Length:240
    Mass (Da):26,117
    Last modified:November 1, 1995 - v1
    Checksum:i22C5614F06D56296
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA89952.1.
    PIRiS55480.
    RefSeqiNP_592815.1. NM_001018215.2.

    Genome annotation databases

    EnsemblFungiiSPAC5H10.02c.1; SPAC5H10.02c.1:pep; SPAC5H10.02c.
    GeneIDi2541799.
    KEGGispo:SPAC5H10.02c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA89952.1.
    PIRiS55480.
    RefSeqiNP_592815.1. NM_001018215.2.

    3D structure databases

    ProteinModelPortaliQ09675.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi278290. 2 interactions.
    MINTiMINT-4693971.

    Protein family/group databases

    MEROPSiC56.A05.

    Proteomic databases

    MaxQBiQ09675.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPAC5H10.02c.1; SPAC5H10.02c.1:pep; SPAC5H10.02c.
    GeneIDi2541799.
    KEGGispo:SPAC5H10.02c.

    Organism-specific databases

    EuPathDBiFungiDB:SPAC5H10.02c.
    PomBaseiSPAC5H10.02c. hsp3102.

    Phylogenomic databases

    HOGENOMiHOG000181653.
    InParanoidiQ09675.
    OMAiASEMGEN.
    OrthoDBiEOG73547H.
    PhylomeDBiQ09675.

    Miscellaneous databases

    PROiQ09675.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    3. "Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe."
      Zhao Q., Su Y., Wang Z., Chen C., Wu T., Huang Y.
      BMC Evol. Biol. 14:86-86(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiHSP32_SCHPO
    AccessioniPrimary (citable) accession number: Q09675
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: June 8, 2016
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.