Reviewed, UniProtKB/Swiss-Prot Q09671 (OYEB_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative NADPH dehydrogenase C5H10.10
    EC=1.6.99.1
Alternative name(s):
    Old yellow enzyme homolog 2

Gene names

ORF Names:

SPAC5H10.10

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor.
Cofactor	FMN.
Subunit structure	Homodimer or heterodimer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Putative NADPH dehydrogenase C5H10.10		PRO_0000194478
Sites
	Binding site	34	1	FMN By similarity
	Binding site	196	1	FMN By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	199	1	Substrate By similarity
	Binding site	249	1	FMN By similarity
	Binding site	341	1	FMN By similarity
	Binding site	368	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09671-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: EE41AD26E3514C4F	FASTA	392	44,983
	10 20 30 40 50 60 MAVQKLYESQ SKLFQPIKVG NMQLQHRMVH APATRLRCLD NGLVMTDLVK EYYKQRSSIP 70 80 90 100 110 120 GTLLITESLF SGAKSGGFSN IPCLYNDEHV EAWKPIVQAI HDNDCFVFIQ FWNLPGELKV 130 140 150 160 170 180 DYLEDQERLE KVTQGECPMD PTGLPAALGS AYSICGEELY VDKFMTKQDI QEHITTYTEA 190 200 210 220 230 240 AKRAVFGCGA DGVEVHQVNG FLLDKFVLNG YGDKCDPEYC GSLENRARFC LELLESVVNA 250 260 270 280 290 300 IGQERVGYRI SPFSDIWKDK DSFEAHVFMI KKIKERFPNL AYLHAIEPRQ YWNGHVHITQ 310 320 330 340 350 360 EKNTLIYKNL WGDPFITAGG HDRDSAIQMA EQENTLVAFG RYFLSNPDLP FRLKYNLPLN 370 380 390 KWDRATFYTK MSPKGYIDYP FSKEFLDTKK VE

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA89960.1.
PIR	S55488.
RefSeq	NP_592823.1.
3D structure databases
HSSP	HSSP built from PDB template 1ICP based on UniProtKB Q9XG54.
ModBase	Search...
Genome annotation databases
GeneID	2541553.
KEGG	spo:SPAC5H10.10.
NMPDR	fig|4896.1.peg.2793.
Organism-specific databases
GeneDB_Spombe	SPAC5H10.10.
Phylogenomic databases
OMA	Q09671. GSAYSIC.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000568-MON.
BRENDA	1.6.99.1. 653.
Gene expression databases
ArrayExpress	Q09671.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OYEB_SCHPO

Accession

Primary (citable) accession number: Q09671

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)