Q09639 (GRK2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G protein-coupled receptor kinase 2
EC=2.7.11.16

Gene names

Name:	grk-2
ORF Names:	W02B3.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically phosphorylates the activated forms of G protein-coupled receptors By similarity.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from mutant phenotype. Source: WormBase hyperosmotic response Inferred from mutant phenotype. Source: WormBase multicellular organismal process Inferred from genetic interaction. Source: WormBase olfactory behavior Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from genetic interaction. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	G protein-coupled receptor kinase 2		PRO_0000086841
Regions
	Domain	54 – 175	122	RGS
	Domain	191 – 455	265	Protein kinase
	Domain	456 – 523	68	AGC-kinase C-terminal
	Domain	558 – 658	101	PH
	Nucleotide binding	197 – 205	9	ATP By similarity
	Region	1 – 190	190	N-terminal
Sites
	Active site	318	1	Proton acceptor By similarity
	Binding site	220	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09639 [UniParc]. Last modified April 23, 2003. Version 2. Checksum: D37F03260F324939	FASTA	707	80,978
	10 20 30 40 50 60 MADLEAVLAD VSYLMAMEKS RSQPAARASK RIVLPDPSVR SIMQKFLEKS GDMKFDKIFN 70 80 90 100 110 120 QKLGFLLLKD YAENVSESPC PQIKFYEAIK EYEKMETPDE RLTKAREIYD HHIMVEMLAH 130 140 150 160 170 180 AHNYSKESLQ HVQYHLLKQN VPPDLFHRYV LEICDQLRGD IFQRFLESDK FTRFCQWKNL 190 200 210 220 230 240 ELNMQLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 250 260 270 280 290 300 IMLSLVSTGQ DCPFIVCMTY AFQSPDKLCF ILDLMNGGDL HYHLSQHGVF TEQEMIFYAS 310 320 330 340 350 360 EVILGLEHMH NRFVVYRDLK PANILLDENG HVRVSDLGLA CDYSKKKPHA SVGTHGYMAP 370 380 390 400 410 420 EVLAKGVAYD SSADWFSLGC MLYKLLKGHS PFRQHKSKDK NEIDKMTLTQ DIELPNEGLS 430 440 450 460 470 480 KDCRDLLEGL LKRDVPDRLG CRGKGPTEVK EHPFFKDVDW QTVYLRRMTP PLIPPRGEVN 490 500 510 520 530 540 AADAFDIGNF DDDEVKGVKL QDGDSDLYKN FNIVISERWQ NEIAETIFEV VNQDADKAES 550 560 570 580 590 600 KKRSKQKIKV AVEEKDSDVI VHGYIKKLGG PFTSAWQTKY GKLYPSRLEL YPESLTAKPE 610 620 630 640 650 660 LVFMDQIEDV CAEMQTIKGE TAIIVKLRDG FKEPKICLTN SDEISLKEWH TSLRTAHKVS 670 680 690 700 QELLQRMGRK AIKIYGVNHD PMLSESERPG SVTRAFLNRA SSVDSGV

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080476 Genomic DNA. Translation: CCD63981.1.
PIR	T26096.
RefSeq	NP_497235.2. NM_064834.3.
UniGene	Cel.9949.
3D structure databases
ProteinModelPortal	Q09639.
SMR	Q09639. Positions 28-667.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1795241.
STRING	Q09639.
Proteomic databases
PRIDE	Q09639.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	W02B3.2; W02B3.2; W02B3.2.
GeneID	175223.
KEGG	cel:W02B3.2.
NMPDR	fig|6239.3.peg.8512.
UCSC	W02B3.2. c. elegans.
Organism-specific databases
CTD	175223.
WormBase	W02B3.2; CE32946; WBGene00001709; grk-2.
Phylogenomic databases
eggNOG	meNOG05348.
GeneTree	EMGT00050000001096.
HOGENOM	HBG443643.
InParanoid	Q09639.
OMA	DIFQKFM.
PhylomeDB	Q09639.
Gene expression databases
ArrayExpress	Q09639.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K00910.
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	887266.

Entry information

Entry name

GRK2_CAEEL

Accession

Primary (citable) accession number: Q09639

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 23, 2003
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project