G protein-coupled receptor kinase 2 - Caenorhabditis elegans

Preferred order of sections

Names and origin

Protein names

Recommended name:
G protein-coupled receptor kinase 2
EC=2.7.11.16

Gene names

Name:	grk-2
ORF Names:	W02B3.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors By similarity.

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from mutant phenotype PubMed 15157420. Source: WormBase hyperosmotic response Inferred from mutant phenotype PubMed 15157420. Source: WormBase multicellular organismal process Inferred from genetic interaction PubMed 15157420. Source: WormBase olfactory behavior Inferred from mutant phenotype PubMed 15157420. Source: WormBase positive regulation of growth rate Inferred from genetic interaction PubMed 15157420. Source: WormBase signal transduction Inferred from electronic annotation. Source: InterPro termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 707

707

G protein-coupled receptor kinase 2

PRO_0000086841

Regions

Domain

54 – 175

122

RGS

Domain

191 – 455

265

Protein kinase

Domain

456 – 523

68

AGC-kinase C-terminal

Domain

558 – 658

101

PH

Nucleotide binding

197 – 205

9

ATP By similarity

Region

1 – 190

190

N-terminal

Sites

Active site

318

1

Proton acceptor By similarity

Binding site

220

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q09639 [UniParc].

Last modified April 23, 2003. Version 2.
Checksum: D37F03260F324939
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FASTA

707

80,978

        10         20         30         40         50         60 
MADLEAVLAD VSYLMAMEKS RSQPAARASK RIVLPDPSVR SIMQKFLEKS GDMKFDKIFN 

        70         80         90        100        110        120 
QKLGFLLLKD YAENVSESPC PQIKFYEAIK EYEKMETPDE RLTKAREIYD HHIMVEMLAH 

       130        140        150        160        170        180 
AHNYSKESLQ HVQYHLLKQN VPPDLFHRYV LEICDQLRGD IFQRFLESDK FTRFCQWKNL 

       190        200        210        220        230        240 
ELNMQLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 

       250        260        270        280        290        300 
IMLSLVSTGQ DCPFIVCMTY AFQSPDKLCF ILDLMNGGDL HYHLSQHGVF TEQEMIFYAS 

       310        320        330        340        350        360 
EVILGLEHMH NRFVVYRDLK PANILLDENG HVRVSDLGLA CDYSKKKPHA SVGTHGYMAP 

       370        380        390        400        410        420 
EVLAKGVAYD SSADWFSLGC MLYKLLKGHS PFRQHKSKDK NEIDKMTLTQ DIELPNEGLS 

       430        440        450        460        470        480 
KDCRDLLEGL LKRDVPDRLG CRGKGPTEVK EHPFFKDVDW QTVYLRRMTP PLIPPRGEVN 

       490        500        510        520        530        540 
AADAFDIGNF DDDEVKGVKL QDGDSDLYKN FNIVISERWQ NEIAETIFEV VNQDADKAES 

       550        560        570        580        590        600 
KKRSKQKIKV AVEEKDSDVI VHGYIKKLGG PFTSAWQTKY GKLYPSRLEL YPESLTAKPE 

       610        620        630        640        650        660 
LVFMDQIEDV CAEMQTIKGE TAIIVKLRDG FKEPKICLTN SDEISLKEWH TSLRTAHKVS 

       670        680        690        700 
QELLQRMGRK AIKIYGVNHD PMLSESERPG SVTRAFLNRA SSVDSGV

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References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080476 Genomic DNA. Translation: CCD63981.1.
PIR	T26096.
RefSeq	NP_497235.2. NM_064834.4.
3D structure databases
ProteinModelPortal	Q09639.
SMR	Q09639. Positions 30-667.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1795241.
STRING	6239.W02B3.2.
Proteomic databases
PaxDb	Q09639.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	W02B3.2; W02B3.2; W02B3.2.
GeneID	175223.
KEGG	cel:CELE_W02B3.2.
UCSC	W02B3.2. c. elegans.
Organism-specific databases
CTD	175223.
WormBase	W02B3.2; CE32946; WBGene00001709; grk-2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00550000074318.
HOGENOM	HOG000006742.
InParanoid	Q09639.
KO	K00910.
OMA	DIFQKFM.
Family and domain databases
Gene3D	2.30.29.30. 2 hits.
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	887266.

Entry information

Entry name

GRK2_CAEEL

Accession

Primary (citable) accession number: Q09639

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 23, 2003
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents