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Protein

Nucleoporin NUP53

Gene

npp-19

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC) (By similarity). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors (By similarity). Required for the proper organization of chromosomes on the mitotic spindle during anaphase (PubMed:28122936).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: UniProtKB
  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: GO_Central
  • nuclear pore organization Source: GO_Central
  • nucleus organization Source: UniProtKB
  • regulation of exit from mitosis Source: UniProtKB
  • regulation of transcription, DNA-templated Source: GO_Central

Keywordsi

Biological processCell cycle, Cell division, Mitosis, mRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP53
Alternative name(s):
35 kDa nucleoporin
Nuclear pore complex protein Nup53
Nucleoporin npp-19
Gene namesi
Name:npp-19
ORF Names:R06F6.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiR06F6.5a; CE01621; WBGene00003805; npp-19.
R06F6.5b; CE18121; WBGene00003805; npp-19.

Subcellular locationi

  • Nucleusnuclear pore complex By similarity
  • Nucleus membrane 1 Publication

GO - Cellular componenti

  • nuclear envelope Source: WormBase
  • nuclear pore Source: UniProtKB
  • nuclear pore central transport channel Source: GO_Central
  • nuclear pore nuclear basket Source: GO_Central

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in embryonic lethality (PubMed:12937276, PubMed:28122936). Also causes severe defects in mitosis due to abnormal chromosome organization on the mitotic spindle during anaphase (PubMed:28122936). Two-cell embryos appear to lack nuclei and pronuclei.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000654181 – 381Nucleoporin NUP53Add BLAST381

Proteomic databases

EPDiQ09601.
PaxDbiQ09601.
PeptideAtlasiQ09601.

PTM databases

iPTMnetiQ09601.

Expressioni

Developmental stagei

Expressed in embryos (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00003805.

Interactioni

Protein-protein interaction databases

BioGridi39976. 19 interactors.
IntActiQ09601. 19 interactors.
STRINGi6239.R06F6.5b.1.

Structurei

3D structure databases

ProteinModelPortaliQ09601.
SMRiQ09601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini195 – 275RRM Nup35-typePROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Belongs to the Nup53 family.Curated

Phylogenomic databases

eggNOGiKOG4285. Eukaryota.
ENOG4111ARE. LUCA.
GeneTreeiENSGT00390000005923.
HOGENOMiHOG000016785.
InParanoidiQ09601.
KOiK14313.
OMAiSVHWSPA.
OrthoDBiEOG091G0DAQ.
PhylomeDBiQ09601.

Family and domain databases

InterProiView protein in InterPro
IPR000504. RRM_dom.
IPR007846. RRM_NUP35_dom.
PfamiView protein in Pfam
PF05172. Nup35_RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS51472. RRM_NUP35. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: Q09601-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSHLNQNTS GRHNSMDLNN SSISNFGTPV EQSTPALLFG KRKATVPSSY
60 70 80 90 100
TASPLNTASA PCSDIFAVSA PAVPQHLKDT PGSKSVHWSP SLVQSGEKSA
110 120 130 140 150
AQTQNTPANL SFGGNSSFSA PTKPAPQSIQ TSSFGGQAMH APPLRSLRDK
160 170 180 190 200
VEPAKKISRR NTFTARSTPL STPITQRVTS RLAEAEEQPM EEEADAADTW
210 220 230 240 250
VTVFGFQPSQ VSILLNLFSR HGEVVSHQTP SKGNFIHMRY SCVTHAQQAI
260 270 280 290 300
SRNGTLLDQD TFIGVVQCTN KDVINGSASG IVARSSNIAA AANRSASMYN
310 320 330 340 350
SFVENDMADQ SVNHNENSVL NSSNVFDANN SLNSSRISVR SGVGMRPLAA
360 370 380
DQRTNILQGT PSVRKAPDGL LNKFWNTIGL N
Note: No experimental confirmation available.
Length:381
Mass (Da):40,786
Last modified:October 3, 2006 - v2
Checksum:i209A625812D6A823
GO
Isoform a (identifier: Q09601-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     356-358: Missing.

Note: No experimental confirmation available.
Show »
Length:378
Mass (Da):40,432
Checksum:i02FB08F44B8BDDEC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020780356 – 358Missing in isoform a. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46794 Genomic DNA. Translation: CAA86775.1.
Z46794 Genomic DNA. Translation: CAA86784.1.
PIRiT23976.
T23985.
RefSeqiNP_496324.1. NM_063923.5. [Q09601-1]
NP_496325.1. NM_063924.5. [Q09601-2]
UniGeneiCel.6495.

Genome annotation databases

EnsemblMetazoaiR06F6.5b; R06F6.5b; WBGene00003805. [Q09601-1]
GeneIDi174663.
KEGGicel:CELE_R06F6.5.
UCSCiR06F6.5b. c. elegans. [Q09601-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNUP53_CAEEL
AccessioniPrimary (citable) accession number: Q09601
Secondary accession number(s): O62340
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 3, 2006
Last modified: July 5, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families