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Protein

NADPH--cytochrome P450 reductase

Gene

emb-8

Organism
Caenorhabditis elegans
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei201FMNUniRule annotation1
Binding sitei290NADPUniRule annotation1
Binding sitei416FADUniRule annotation1
Binding sitei470FADUniRule annotation1
Binding sitei520NADPUniRule annotation1
Binding sitei623NADPUniRule annotation1
Binding sitei661FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi79 – 84FMNUniRule annotation6
Nucleotide bindingi131 – 134FMNUniRule annotation4
Nucleotide bindingi166 – 175FMNUniRule annotation10
Nucleotide bindingi446 – 449FADUniRule annotation4
Nucleotide bindingi464 – 466FADUniRule annotation3
Nucleotide bindingi479 – 482FADUniRule annotation4
Nucleotide bindingi581 – 582NADPUniRule annotation2
Nucleotide bindingi587 – 591NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

  • eggshell formation Source: WormBase
  • lipid biosynthetic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Ligandi

FADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

ReactomeiR-CEL-211897. Cytochrome P450 - arranged by substrate type.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:emb-8Imported
ORF Names:CELE_K10D2.6Imported, K10D2.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiK10D2.6; CE02018; WBGene00001262; emb-8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13LumenalUniRule annotationAdd BLAST13
Transmembranei14 – 34HelicalUniRule annotationAdd BLAST21
Topological domaini35 – 662CytoplasmicUniRule annotationAdd BLAST628

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulumUniRule annotation, Membrane

PTM / Processingi

Proteomic databases

EPDiQ09590.
PaxDbiQ09590.
PeptideAtlasiQ09590.

Expressioni

Gene expression databases

BgeeiWBGene00001262.

Interactioni

Protein-protein interaction databases

DIPiDIP-27434N.
IntActiQ09590. 1 interactor.
MINTiMINT-1045559.
STRINGi6239.K10D2.6.1.

Structurei

3D structure databases

ProteinModelPortaliQ09590.
SMRiQ09590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 217Flavodoxin-likeInterPro annotationAdd BLAST145
Domaini270 – 506FAD-binding FR-typeInterPro annotationAdd BLAST237

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOGENOMiHOG000282027.
InParanoidiQ09590.
KOiK00327.
OMAiHIEIDIT.
OrthoDBiEOG091G02VT.
PhylomeDBiQ09590.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAWIVSGLD TSDLVVLTLL AGGAIIFLFM KVFNQQPSSS RYSPTVASVT
60 70 80 90 100
TSAAASKSNQ SFIDRMKNEN RQVLIMYGSQ TGTAEEMSGR LAKDLTRYTK
110 120 130 140 150
KAVVVDPEDI ECEDLNRLSE VEDALLVLCI ATYGEGDPTD NAVTLVEYLN
160 170 180 190 200
AGDCDLSGVR FAVFGLGNKT YEHFNEIGIQ MDKQLEKLGA KRIFHLGLGD
210 220 230 240 250
DDANLEEDFM IWREAFLPKV AEEFGWELNT EAETMRQYQL EPVEEGKALF
260 270 280 290 300
KGEFGRLGAY ERPRPPFDVK NPYLATVAIN DELHTEHSDR SCRHIEFSVE
310 320 330 340 350
GSRIRYEAGD HLAVFPTNDP VLVDRLINML QFDPDHAFRL VNVDEDASKR
360 370 380 390 400
HPFPCPTTFR TALSHYVDIC APVKSHVLKA ISEYCTDDTE KEFLNKLSTA
410 420 430 440 450
NEEGLKEYAR YIVKERRSIV DVLTDQKTCK PPIEYLLELL PRLQARYYSI
460 470 480 490 500
ASSPRLNEEK IAICAVVTKY SIGDRDINGV CTRYLTTKDA GSKSPVFVRK
510 520 530 540 550
STMRLPHRTT TQVIMIGPGT GFAPFRGFLQ DRQFHKNAGK EIGAMHLYYG
560 570 580 590 600
CRHPDHDYIY KDELAKFQED EVLTHLVCAF SRAQEHKIYV QDRLWETRDR
610 620 630 640 650
IWDAINVGAH VYICGDARNM ARDVQATLQK IFREIGGKSE TEAVAYFKDM
660
EKTKRYQADV WS
Length:662
Mass (Da):75,208
Last modified:November 1, 1996 - v1
Checksum:i28271950576B0EE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA. Translation: CCD72861.1.
PIRiG88451.
RefSeqiNP_498103.1. NM_065702.5.
UniGeneiCel.5243.

Genome annotation databases

EnsemblMetazoaiK10D2.6.1; K10D2.6.1; WBGene00001262.
K10D2.6.2; K10D2.6.2; WBGene00001262.
GeneIDi175710.
KEGGicel:CELE_K10D2.6.
UCSCiK10D2.6.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA. Translation: CCD72861.1.
PIRiG88451.
RefSeqiNP_498103.1. NM_065702.5.
UniGeneiCel.5243.

3D structure databases

ProteinModelPortaliQ09590.
SMRiQ09590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27434N.
IntActiQ09590. 1 interactor.
MINTiMINT-1045559.
STRINGi6239.K10D2.6.1.

Proteomic databases

EPDiQ09590.
PaxDbiQ09590.
PeptideAtlasiQ09590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK10D2.6.1; K10D2.6.1; WBGene00001262.
K10D2.6.2; K10D2.6.2; WBGene00001262.
GeneIDi175710.
KEGGicel:CELE_K10D2.6.
UCSCiK10D2.6.1. c. elegans.

Organism-specific databases

CTDi175710.
WormBaseiK10D2.6; CE02018; WBGene00001262; emb-8.

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOGENOMiHOG000282027.
InParanoidiQ09590.
KOiK00327.
OMAiHIEIDIT.
OrthoDBiEOG091G02VT.
PhylomeDBiQ09590.

Enzyme and pathway databases

ReactomeiR-CEL-211897. Cytochrome P450 - arranged by substrate type.

Miscellaneous databases

PROiQ09590.

Gene expression databases

BgeeiWBGene00001262.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ09590_CAEEL
AccessioniPrimary (citable) accession number: Q09590
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.