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Protein

Transcription factor aptf-1

Gene

aptf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor, which is required in the single sleep-active ring interneuron RIS for sleep-like behavioral quiescence induced by neuropeptide signaling in larvae (PubMed:24184105, PubMed:26949257). Regulates gene expression of sleep-inducing FMRFamide-like neuropeptide flp-11 in RIS (PubMed:26949257).2 Publications

GO - Molecular functioni

GO - Biological processi

  • anatomical structure development Source: GO_Central
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • regulation of cell proliferation Source: GO_Central
  • sleep Source: WormBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-CEL-3232118. SUMOylation of transcription factors.
R-CEL-8866904. Negative regulation of activity of TFAP2 (AP-2) family transcription factors.
R-CEL-8866907. Activation of the TFAP2 (AP-2) family of transcription factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor aptf-11 Publication
Alternative name(s):
AP2 transcription factor aptf-11 Publication
Gene namesi
Name:aptf-11 PublicationImported
ORF Names:K06A1.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiK06A1.1; CE35990; WBGene00019424; aptf-1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Animals develop with normal speed and go normally through the molt. They stop feeding for a normal time before each molt, but average nose speed is reduced by only about 10% and unlike wild-type, they are never immobile. The command interneurons AVA and AVE, which control backward movement, are not shut down during sleep-like behavior. Optogenetic channelrhodopsin activation of AIBs, RIBs and RIS interneurons during wake-like behavior using blue light does not trigger acute immobility and cessation of pumping in L1 larvae, instead it causes an increase in mobility (PubMed:24184105). No change in GABAergic function of neurons as unc-25 and the vesicular GABA transporter gene unc-47 are expressed normally. The expression of a neuropeptide gene flp-11 is strongly down-regulated in pretzel-stage embryos and in sleeping L4 larvae (PubMed:26949257).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004375441 – 365Transcription factor aptf-1Add BLAST365

Proteomic databases

PaxDbiQ09585.

Expressioni

Tissue specificityi

Expressed in five interneurons AIB, RIB and RIS.1 Publication

Developmental stagei

Expressed strongly during late embryogenesis and early larval development in three types of interneurons, the paired neurons AIB and RIB and the unpaired neuron RIS in the head, with the strongest expression in RIS. Expression after embryonic development decreases until the adult stage.1 Publication

Inductioni

Regulates its own expression during larval development (PubMed:24184105). Transcriptionally regulated by transcription factor lim-6 in RIS interneuron in all developmental stages (PubMed:26949257).2 Publications

Gene expression databases

BgeeiWBGene00019424.

Interactioni

Subunit structurei

Binds DNA as a dimer.By similarity

Protein-protein interaction databases

STRINGi6239.K06A1.1.

Structurei

3D structure databases

ProteinModelPortaliQ09585.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni223 – 356H-S-H (helix-span-helix), dimerizationSequence analysisAdd BLAST134

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi96 – 101Poly-GlnSequence analysis6

Sequence similaritiesi

Belongs to the AP-2 family.Curated

Phylogenomic databases

eggNOGiKOG3811. Eukaryota.
ENOG410XR9E. LUCA.
GeneTreeiENSGT00550000074577.
HOGENOMiHOG000016929.
InParanoidiQ09585.
KOiK09180.
OMAiSQCDEED.
OrthoDBiEOG091G0PR6.
PhylomeDBiQ09585.

Family and domain databases

InterProiIPR004979. TF_AP2.
IPR013854. TF_AP2_C.
[Graphical view]
PANTHERiPTHR10812. PTHR10812. 2 hits.
PfamiPF03299. TF_AP-2. 1 hit.
[Graphical view]
PRINTSiPR01748. AP2TNSCPFCT.

Sequencei

Sequence statusi: Complete.

Q09585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNRKLMECS DDEFVRGSQC DEEDEEEQQR QSSQRPPFYE NMRTFAPSHM
60 70 80 90 100
HISCHYDIPY PPHLIQPPVC GVSTFTYARE FPVSNSQQFF NVTPPQQQQQ
110 120 130 140 150
QGTGGSGEED YCIPKSEDRD HSSNYSQVAT KQDRPFVFFA SDCQSPLEIV
160 170 180 190 200
GEVYCTVPGR TSLLSSTTKY RVTVAEIQRR ISPPECLNAS LLGGILRKAK
210 220 230 240 250
SKDGGKTLRD SLKKLGLTLP AGRRKQANVT AWTALVEEEA IHMAKEFALV
260 270 280 290 300
CEKEFHSREI GIYLTKTSLA IDPDVVKRRT ALEMSRKVVG ELAELLSCDR
310 320 330 340 350
TPLTPYFPRN MLPIDPSVQQ HLSHFTLMTH GFGNVAMSAV LESVKLMIDE
360
SIKYIDRCCS QNVWR
Length:365
Mass (Da):41,328
Last modified:March 1, 2004 - v2
Checksum:i8E25A54BA9953E8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284602 Genomic DNA. Translation: CCD72609.1.
PIRiT34333.
RefSeqiNP_495300.2. NM_062899.2.
UniGeneiCel.14799.

Genome annotation databases

EnsemblMetazoaiK06A1.1; K06A1.1; WBGene00019424.
GeneIDi187043.
KEGGicel:CELE_K06A1.1.
UCSCiK06A1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284602 Genomic DNA. Translation: CCD72609.1.
PIRiT34333.
RefSeqiNP_495300.2. NM_062899.2.
UniGeneiCel.14799.

3D structure databases

ProteinModelPortaliQ09585.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K06A1.1.

Proteomic databases

PaxDbiQ09585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK06A1.1; K06A1.1; WBGene00019424.
GeneIDi187043.
KEGGicel:CELE_K06A1.1.
UCSCiK06A1.1. c. elegans.

Organism-specific databases

CTDi187043.
WormBaseiK06A1.1; CE35990; WBGene00019424; aptf-1.

Phylogenomic databases

eggNOGiKOG3811. Eukaryota.
ENOG410XR9E. LUCA.
GeneTreeiENSGT00550000074577.
HOGENOMiHOG000016929.
InParanoidiQ09585.
KOiK09180.
OMAiSQCDEED.
OrthoDBiEOG091G0PR6.
PhylomeDBiQ09585.

Enzyme and pathway databases

ReactomeiR-CEL-3232118. SUMOylation of transcription factors.
R-CEL-8866904. Negative regulation of activity of TFAP2 (AP-2) family transcription factors.
R-CEL-8866907. Activation of the TFAP2 (AP-2) family of transcription factors.

Gene expression databases

BgeeiWBGene00019424.

Family and domain databases

InterProiIPR004979. TF_AP2.
IPR013854. TF_AP2_C.
[Graphical view]
PANTHERiPTHR10812. PTHR10812. 2 hits.
PfamiPF03299. TF_AP-2. 1 hit.
[Graphical view]
PRINTSiPR01748. AP2TNSCPFCT.
ProtoNetiSearch...

Entry informationi

Entry nameiAPTF1_CAEEL
AccessioniPrimary (citable) accession number: Q09585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: March 1, 2004
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.