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Q09582

- METH_CAEEL

UniProt

Q09582 - METH_CAEEL

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Protein

Probable methionine synthase

Gene

metr-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).By similarity

Catalytic activityi

5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.

Cofactori

Methylcobalamin (MeCBL).By similarity
Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi249 – 2491ZincPROSITE-ProRule annotation
Metal bindingi312 – 3121ZincPROSITE-ProRule annotation
Metal bindingi313 – 3131ZincPROSITE-ProRule annotation
Metal bindingi775 – 7751Cobalt (cobalamin axial ligand)By similarity
Binding sitei820 – 8201CobalaminBy similarity
Binding sitei963 – 9631S-adenosyl-L-methionineBy similarity
Binding sitei1157 – 11571S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei1161 – 11611Cobalamin; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. methionine synthase activity Source: UniProtKB-EC
  3. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. pteridine-containing compound metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Cobalamin, Cobalt, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiREACT_183520. Sulfur amino acid metabolism.
REACT_184618. Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE.
REACT_184623. Defective MTR causes methylmalonic aciduria and homocystinuria type cblG.
REACT_184630. Cobalamin (Cbl, vitamin B12) transport and metabolism.
UniPathwayiUPA00051; UER00081.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methionine synthase (EC:2.1.1.13)
Alternative name(s):
5-methyltetrahydrofolate--homocysteine methyltransferase
Vitamin-B12 dependent methionine synthase
Short name:
MS
Gene namesi
Name:metr-1
ORF Names:R03D7.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiR03D7.1; CE01609; WBGene00010988; metr-1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12491249Probable methionine synthasePRO_0000204531Add
BLAST

Proteomic databases

PaxDbiQ09582.
PRIDEiQ09582.

Interactioni

Protein-protein interaction databases

BioGridi39991. 1 interaction.
DIPiDIP-26122N.
IntActiQ09582. 1 interaction.
MINTiMINT-1116270.
STRINGi6239.R03D7.1.

Structurei

3D structure databases

ProteinModelPortaliQ09582.
SMRiQ09582. Positions 5-640, 655-1249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 327327Hcy-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini360 – 621262Pterin-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini652 – 74998B12-binding N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini762 – 897136B12-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini913 – 1249337AdoMet activationPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni850 – 8512Cobalamin-bindingBy similarity
Regioni1211 – 12122S-adenosyl-L-methionine bindingBy similarity

Domaini

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin (By similarity).By similarity

Sequence similaritiesi

Contains 1 AdoMet activation domain.PROSITE-ProRule annotation
Contains 1 B12-binding domain.PROSITE-ProRule annotation
Contains 1 Hcy-binding domain.PROSITE-ProRule annotation
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1410.
GeneTreeiENSGT00420000029824.
HOGENOMiHOG000251409.
InParanoidiQ09582.
KOiK00548.
OMAiXAEKDSA.
OrthoDBiEOG7TF786.
PhylomeDBiQ09582.

Family and domain databases

Gene3Di1.10.1240.10. 1 hit.
3.10.196.10. 1 hit.
3.20.20.20. 1 hit.
3.20.20.330. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR003759. Cbl-bd_cap.
IPR006158. Cobalamin-bd.
IPR011005. Dihydropteroate_synth-like.
IPR011822. MetH.
IPR000489. Pterin-binding.
IPR003726. S_MeTrfase.
IPR004223. VitB12-dep_Met_synth_activ_dom.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF02607. B12-binding_2. 1 hit.
PF02965. Met_synt_B12. 1 hit.
PF00809. Pterin_bind. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF000381. MetH. 1 hit.
SMARTiSM01018. B12-binding_2. 1 hit.
[Graphical view]
SUPFAMiSSF47644. SSF47644. 1 hit.
SSF51717. SSF51717. 1 hit.
SSF52242. SSF52242. 1 hit.
SSF56507. SSF56507. 1 hit.
SSF82282. SSF82282. 1 hit.
TIGRFAMsiTIGR02082. metH. 1 hit.
PROSITEiPS50974. ADOMET_ACTIVATION. 1 hit.
PS51332. B12_BINDING. 1 hit.
PS51337. B12_BINDING_NTER. 1 hit.
PS50970. HCY. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09582-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRSSLFEEL AEIAKERIMI IDGAMGTMIQ REYMEEEDFR GEILKDHDKP
60 70 80 90 100
LKGNNDLLSI TRPDIIYKIH KLYLEAGADF VETNTFSGTT IAQADYRCEH
110 120 130 140 150
LVHEINYQSA LVARRACDDV GAATGRRRYV CGAIGPTNRT LSISPSVEKP
160 170 180 190 200
DFRNVTFQEL VKAYGDQARS LIQGGVDVLL VETVFDSANA KAALFAIRTL
210 220 230 240 250
FEDEGVPEMP VFLSGTIVDM SGRTLSGQTG EAFLVSTKQG KPMAVGLNCA
260 270 280 290 300
LGAKDMRQFV DNMSKWSDSF IICYPNAGLP NALGGYDETP EEMADVLREF
310 320 330 340 350
ARDGLVNIIG GCCGTTPDHI NAMYKAVQGI TPRVPPQDPH AGKMLLSGLE
360 370 380 390 400
PSIVGPETNF VNIGERCNVA GSRRFCNLIK NENYDTAIDV ARVQVDSGAQ
410 420 430 440 450
ILDVNMDDGL LDGPYAMSKF LRLISSEPDV AKIPVCIDSS DFDVIIAGLE
460 470 480 490 500
STQGKCVVNS ISLKEGEEKF KERARIIKRY GAAVVVMAFD EQGQAAETDP
510 520 530 540 550
KFEICERSYR ILTEEVGFNP NDIIFDANIL TIATGMEEHS NYGMYFIEAA
560 570 580 590 600
RMIRENLPGA HVSGGVSNIS FSFRGMEAIR EAMHSVFLFY AIKAGMDMGI
610 620 630 640 650
VNAGALPVYE DIDKPLLQLL EDLLFNRDPE ATEKLLVAAQ EMKKDGKKAD
660 670 680 690 700
TKTDEWRSLT VEERLKFALV KGVDQFVVAD TEEARQNTAK YPRPLNVIER
710 720 730 740 750
PLMDGMAVVG ELFGAGKMFL PQVIKSARVM KKAVAHLLPF MEIERQANIE
760 770 780 790 800
TMGLAEDESP YQGTVVIATV KGDVHDIGKN IVSVVLGCNN FKVVDLGVMT
810 820 830 840 850
PCENIIKAAI EEKADFIGLS GLITPSLDEM VYVAKEMNRV GLNIPLLIGG
860 870 880 890 900
ATTSKTHTAV KISPRYPHPV VHCLDASKSV VVCSSLSDMS VRDAFLQDLN
910 920 930 940 950
EDYEDVRQEH YASLKDRRFT DLNKTREKKF KIDWDKFTAV KPSFVGRREY
960 970 980 990 1000
QNFDLNELIP YIDWKPFFDV WQLRGKYPNR SYPKIFDDAD VGAEAKKVFD
1010 1020 1030 1040 1050
DAQTWLKKLI DEKILVANAV VSFLPAASEG DDMHVYDPET GNKLDTFYGL
1060 1070 1080 1090 1100
RQQSGREHDQ PHFCLSDFIK PLKNGVPDDY LGLFACTAGL GAEEYCKTLE
1110 1120 1130 1140 1150
KNHDDYASIM VKALADRLAE AYAEYLHKEV RTTLWGYSTN EDLTESDLLS
1160 1170 1180 1190 1200
IKYQGIRPAC GYPSQPDHTE KRTLWKLLEA EKNGIGLTEH LAMLPAASVS
1210 1220 1230 1240
GLYFANPQSE YFAVGKIDQD QVIDYAARKN VPKEEVERWL SPILGYDTD
Length:1,249
Mass (Da):138,899
Last modified:November 1, 1996 - v1
Checksum:i4D16028AD02CD550
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46828 Genomic DNA. Translation: CAA86855.1.
PIRiT23868.
RefSeqiNP_496353.1. NM_063952.4.
UniGeneiCel.16853.

Genome annotation databases

EnsemblMetazoaiR03D7.1; R03D7.1; WBGene00010988.
GeneIDi174681.
KEGGicel:CELE_R03D7.1.
UCSCiR03D7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46828 Genomic DNA. Translation: CAA86855.1 .
PIRi T23868.
RefSeqi NP_496353.1. NM_063952.4.
UniGenei Cel.16853.

3D structure databases

ProteinModelPortali Q09582.
SMRi Q09582. Positions 5-640, 655-1249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 39991. 1 interaction.
DIPi DIP-26122N.
IntActi Q09582. 1 interaction.
MINTi MINT-1116270.
STRINGi 6239.R03D7.1.

Proteomic databases

PaxDbi Q09582.
PRIDEi Q09582.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai R03D7.1 ; R03D7.1 ; WBGene00010988 .
GeneIDi 174681.
KEGGi cel:CELE_R03D7.1.
UCSCi R03D7.1. c. elegans.

Organism-specific databases

CTDi 174681.
WormBasei R03D7.1 ; CE01609 ; WBGene00010988 ; metr-1.

Phylogenomic databases

eggNOGi COG1410.
GeneTreei ENSGT00420000029824.
HOGENOMi HOG000251409.
InParanoidi Q09582.
KOi K00548.
OMAi XAEKDSA.
OrthoDBi EOG7TF786.
PhylomeDBi Q09582.

Enzyme and pathway databases

UniPathwayi UPA00051 ; UER00081 .
Reactomei REACT_183520. Sulfur amino acid metabolism.
REACT_184618. Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE.
REACT_184623. Defective MTR causes methylmalonic aciduria and homocystinuria type cblG.
REACT_184630. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

NextBioi 885042.
PROi Q09582.

Family and domain databases

Gene3Di 1.10.1240.10. 1 hit.
3.10.196.10. 1 hit.
3.20.20.20. 1 hit.
3.20.20.330. 1 hit.
3.40.50.280. 1 hit.
InterProi IPR003759. Cbl-bd_cap.
IPR006158. Cobalamin-bd.
IPR011005. Dihydropteroate_synth-like.
IPR011822. MetH.
IPR000489. Pterin-binding.
IPR003726. S_MeTrfase.
IPR004223. VitB12-dep_Met_synth_activ_dom.
[Graphical view ]
Pfami PF02310. B12-binding. 1 hit.
PF02607. B12-binding_2. 1 hit.
PF02965. Met_synt_B12. 1 hit.
PF00809. Pterin_bind. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view ]
PIRSFi PIRSF000381. MetH. 1 hit.
SMARTi SM01018. B12-binding_2. 1 hit.
[Graphical view ]
SUPFAMi SSF47644. SSF47644. 1 hit.
SSF51717. SSF51717. 1 hit.
SSF52242. SSF52242. 1 hit.
SSF56507. SSF56507. 1 hit.
SSF82282. SSF82282. 1 hit.
TIGRFAMsi TIGR02082. metH. 1 hit.
PROSITEi PS50974. ADOMET_ACTIVATION. 1 hit.
PS51332. B12_BINDING. 1 hit.
PS51337. B12_BINDING_NTER. 1 hit.
PS50970. HCY. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiMETH_CAEEL
AccessioniPrimary (citable) accession number: Q09582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

L-homocysteine is bound via the zinc atom.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3