SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q09582

- METH_CAEEL

UniProt

Q09582 - METH_CAEEL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Probable methionine synthase
Gene
metr-1, R03D7.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate By similarity.

Catalytic activityi

5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.

Cofactori

Methylcobalamin (MeCBL) By similarity.
Binds 1 zinc ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi249 – 2491Zinc By similarity
Metal bindingi312 – 3121Zinc By similarity
Metal bindingi313 – 3131Zinc By similarity
Metal bindingi775 – 7751Cobalt (cobalamin axial ligand) By similarity
Binding sitei820 – 8201Cobalamin By similarity
Binding sitei963 – 9631S-adenosyl-L-methionine By similarity
Binding sitei1157 – 11571S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei1161 – 11611Cobalamin; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro
  2. cobalamin binding Source: UniProtKB-KW
  3. methionine synthase activity Source: UniProtKB-EC
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. pteridine-containing compound metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Cobalamin, Cobalt, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiREACT_183520. Sulfur amino acid metabolism.
REACT_184618. Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE.
REACT_184623. Defective MTR causes methylmalonic aciduria and homocystinuria type cblG.
REACT_184630. Cobalamin (Cbl, vitamin B12) transport and metabolism.
UniPathwayiUPA00051; UER00081.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methionine synthase (EC:2.1.1.13)
Alternative name(s):
5-methyltetrahydrofolate--homocysteine methyltransferase
Vitamin-B12 dependent methionine synthase
Short name:
MS
Gene namesi
Name:metr-1
ORF Names:R03D7.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiR03D7.1; CE01609; WBGene00010988; metr-1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12491249Probable methionine synthase
PRO_0000204531Add
BLAST

Proteomic databases

PaxDbiQ09582.
PRIDEiQ09582.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Y76A2B.5Q9XWC91EBI-331777,EBI-331773

Protein-protein interaction databases

BioGridi39991. 1 interaction.
DIPiDIP-26122N.
IntActiQ09582. 1 interaction.
MINTiMINT-1116270.
STRINGi6239.R03D7.1.

Structurei

3D structure databases

ProteinModelPortaliQ09582.
SMRiQ09582. Positions 5-640, 655-1249.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 327327Hcy-binding
Add
BLAST
Domaini360 – 621262Pterin-binding
Add
BLAST
Domaini652 – 74998B12-binding N-terminal
Add
BLAST
Domaini762 – 897136B12-binding
Add
BLAST
Domaini913 – 1249337AdoMet activation
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni850 – 8512Cobalamin-binding By similarity
Regioni1211 – 12122S-adenosyl-L-methionine binding By similarity

Domaini

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin By similarity.

Sequence similaritiesi

Contains 1 B12-binding domain.
Contains 1 Hcy-binding domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1410.
GeneTreeiENSGT00420000029824.
HOGENOMiHOG000251409.
InParanoidiQ09582.
KOiK00548.
OMAiXAEKDSA.
OrthoDBiEOG7TF786.
PhylomeDBiQ09582.

Family and domain databases

Gene3Di1.10.1240.10. 1 hit.
3.10.196.10. 1 hit.
3.20.20.20. 1 hit.
3.20.20.330. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR003759. Cbl-bd_cap.
IPR006158. Cobalamin-bd.
IPR011005. Dihydropteroate_synth-like.
IPR011822. MetH.
IPR000489. Pterin-binding.
IPR003726. S_MeTrfase.
IPR004223. VitB12-dep_Met_synth_activ_dom.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF02607. B12-binding_2. 1 hit.
PF02965. Met_synt_B12. 1 hit.
PF00809. Pterin_bind. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF000381. MetH. 1 hit.
SMARTiSM01018. B12-binding_2. 1 hit.
[Graphical view]
SUPFAMiSSF47644. SSF47644. 1 hit.
SSF51717. SSF51717. 1 hit.
SSF52242. SSF52242. 1 hit.
SSF56507. SSF56507. 1 hit.
SSF82282. SSF82282. 1 hit.
TIGRFAMsiTIGR02082. metH. 1 hit.
PROSITEiPS50974. ADOMET_ACTIVATION. 1 hit.
PS51332. B12_BINDING. 1 hit.
PS51337. B12_BINDING_NTER. 1 hit.
PS50970. HCY. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09582-1 [UniParc]FASTAAdd to Basket

« Hide

MTRSSLFEEL AEIAKERIMI IDGAMGTMIQ REYMEEEDFR GEILKDHDKP     50
LKGNNDLLSI TRPDIIYKIH KLYLEAGADF VETNTFSGTT IAQADYRCEH 100
LVHEINYQSA LVARRACDDV GAATGRRRYV CGAIGPTNRT LSISPSVEKP 150
DFRNVTFQEL VKAYGDQARS LIQGGVDVLL VETVFDSANA KAALFAIRTL 200
FEDEGVPEMP VFLSGTIVDM SGRTLSGQTG EAFLVSTKQG KPMAVGLNCA 250
LGAKDMRQFV DNMSKWSDSF IICYPNAGLP NALGGYDETP EEMADVLREF 300
ARDGLVNIIG GCCGTTPDHI NAMYKAVQGI TPRVPPQDPH AGKMLLSGLE 350
PSIVGPETNF VNIGERCNVA GSRRFCNLIK NENYDTAIDV ARVQVDSGAQ 400
ILDVNMDDGL LDGPYAMSKF LRLISSEPDV AKIPVCIDSS DFDVIIAGLE 450
STQGKCVVNS ISLKEGEEKF KERARIIKRY GAAVVVMAFD EQGQAAETDP 500
KFEICERSYR ILTEEVGFNP NDIIFDANIL TIATGMEEHS NYGMYFIEAA 550
RMIRENLPGA HVSGGVSNIS FSFRGMEAIR EAMHSVFLFY AIKAGMDMGI 600
VNAGALPVYE DIDKPLLQLL EDLLFNRDPE ATEKLLVAAQ EMKKDGKKAD 650
TKTDEWRSLT VEERLKFALV KGVDQFVVAD TEEARQNTAK YPRPLNVIER 700
PLMDGMAVVG ELFGAGKMFL PQVIKSARVM KKAVAHLLPF MEIERQANIE 750
TMGLAEDESP YQGTVVIATV KGDVHDIGKN IVSVVLGCNN FKVVDLGVMT 800
PCENIIKAAI EEKADFIGLS GLITPSLDEM VYVAKEMNRV GLNIPLLIGG 850
ATTSKTHTAV KISPRYPHPV VHCLDASKSV VVCSSLSDMS VRDAFLQDLN 900
EDYEDVRQEH YASLKDRRFT DLNKTREKKF KIDWDKFTAV KPSFVGRREY 950
QNFDLNELIP YIDWKPFFDV WQLRGKYPNR SYPKIFDDAD VGAEAKKVFD 1000
DAQTWLKKLI DEKILVANAV VSFLPAASEG DDMHVYDPET GNKLDTFYGL 1050
RQQSGREHDQ PHFCLSDFIK PLKNGVPDDY LGLFACTAGL GAEEYCKTLE 1100
KNHDDYASIM VKALADRLAE AYAEYLHKEV RTTLWGYSTN EDLTESDLLS 1150
IKYQGIRPAC GYPSQPDHTE KRTLWKLLEA EKNGIGLTEH LAMLPAASVS 1200
GLYFANPQSE YFAVGKIDQD QVIDYAARKN VPKEEVERWL SPILGYDTD 1249
Length:1,249
Mass (Da):138,899
Last modified:November 1, 1996 - v1
Checksum:i4D16028AD02CD550
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46828 Genomic DNA. Translation: CAA86855.1.
PIRiT23868.
RefSeqiNP_496353.1. NM_063952.4.
UniGeneiCel.16853.

Genome annotation databases

EnsemblMetazoaiR03D7.1; R03D7.1; WBGene00010988.
GeneIDi174681.
KEGGicel:CELE_R03D7.1.
UCSCiR03D7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46828 Genomic DNA. Translation: CAA86855.1 .
PIRi T23868.
RefSeqi NP_496353.1. NM_063952.4.
UniGenei Cel.16853.

3D structure databases

ProteinModelPortali Q09582.
SMRi Q09582. Positions 5-640, 655-1249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 39991. 1 interaction.
DIPi DIP-26122N.
IntActi Q09582. 1 interaction.
MINTi MINT-1116270.
STRINGi 6239.R03D7.1.

Proteomic databases

PaxDbi Q09582.
PRIDEi Q09582.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai R03D7.1 ; R03D7.1 ; WBGene00010988 .
GeneIDi 174681.
KEGGi cel:CELE_R03D7.1.
UCSCi R03D7.1. c. elegans.

Organism-specific databases

CTDi 174681.
WormBasei R03D7.1 ; CE01609 ; WBGene00010988 ; metr-1.

Phylogenomic databases

eggNOGi COG1410.
GeneTreei ENSGT00420000029824.
HOGENOMi HOG000251409.
InParanoidi Q09582.
KOi K00548.
OMAi XAEKDSA.
OrthoDBi EOG7TF786.
PhylomeDBi Q09582.

Enzyme and pathway databases

UniPathwayi UPA00051 ; UER00081 .
Reactomei REACT_183520. Sulfur amino acid metabolism.
REACT_184618. Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE.
REACT_184623. Defective MTR causes methylmalonic aciduria and homocystinuria type cblG.
REACT_184630. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

NextBioi 885042.
PROi Q09582.

Family and domain databases

Gene3Di 1.10.1240.10. 1 hit.
3.10.196.10. 1 hit.
3.20.20.20. 1 hit.
3.20.20.330. 1 hit.
3.40.50.280. 1 hit.
InterProi IPR003759. Cbl-bd_cap.
IPR006158. Cobalamin-bd.
IPR011005. Dihydropteroate_synth-like.
IPR011822. MetH.
IPR000489. Pterin-binding.
IPR003726. S_MeTrfase.
IPR004223. VitB12-dep_Met_synth_activ_dom.
[Graphical view ]
Pfami PF02310. B12-binding. 1 hit.
PF02607. B12-binding_2. 1 hit.
PF02965. Met_synt_B12. 1 hit.
PF00809. Pterin_bind. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view ]
PIRSFi PIRSF000381. MetH. 1 hit.
SMARTi SM01018. B12-binding_2. 1 hit.
[Graphical view ]
SUPFAMi SSF47644. SSF47644. 1 hit.
SSF51717. SSF51717. 1 hit.
SSF52242. SSF52242. 1 hit.
SSF56507. SSF56507. 1 hit.
SSF82282. SSF82282. 1 hit.
TIGRFAMsi TIGR02082. metH. 1 hit.
PROSITEi PS50974. ADOMET_ACTIVATION. 1 hit.
PS51332. B12_BINDING. 1 hit.
PS51337. B12_BINDING_NTER. 1 hit.
PS50970. HCY. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiMETH_CAEEL
AccessioniPrimary (citable) accession number: Q09582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

L-homocysteine is bound via the zinc atom By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi