Putative folylpolyglutamate synthase - Caenorhabditis elegans

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Q09509 (FOLC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 77. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative folylpolyglutamate synthase
EC=6.3.2.17

Alternative name(s):
Folylpoly-gamma-glutamate synthetase
Short name=FPGS
Tetrahydrofolylpolyglutamate synthase
Short name=Tetrahydrofolate synthase

Gene names

ORF Names:

F25B5.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis By similarity.
Catalytic activity	ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).
Cofactor	A monovalent cation By similarity.
Pathway	Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
Subcellular location	Mitochondrion inner membrane By similarity. Mitochondrion matrix By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative initiation Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform a (identifier: Q09509-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.

Isoform b (identifier: Q09509-2) The sequence of this isoform differs from the canonical sequence as follows: 3-9: LLPQTNR → IISSIFAPTRGFHSRSTW
Note: No experimental confirmation available. Produced by alternative splicing.

Isoform c (identifier: Q09509-3) The sequence of this isoform differs from the canonical sequence as follows: 1-26: Missing.
Note: No experimental confirmation available. Produced by alternative initiation at Met-27 of isoform a.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 510

510

Putative folylpolyglutamate synthase

PRO_0000168306

Regions

Nucleotide binding

95 – 101

ATP Potential

Natural variations

Alternative sequence

1 – 26

Missing in isoform c.

VSP_041963

Alternative sequence

3 – 9

LLPQTNR → IISSIFAPTRGFHSRSTW in isoform b.

VSP_041964

Sequences

Sequence		Length	Mass (Da)
Isoform a [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 12A965157DFED84A Show »	FASTA	510	56,433
10 20 30 40 50 60 MRLLPQTNRI LLPTTSSTAC GANQLRMSSE KAVPCYEESV RLLNGLQSNA ATIKKLRVQR 70 80 90 100 110 120 ENLQAINLPQ CRKYLESLNI SAEDLNALNI IHVSGTKGKG SACAFVESIL RSQGLRTGFY 130 140 150 160 170 180 SSPHLVHVRE RIQVDGQPVS EQMFAEEFFH VYDIIKREHS DNMPAYFKFL TLLAFRIFVK 190 200 210 220 230 240 LNVQVMILEV GIGGEYDCTN VVEKPKVCGV TTLDYDHMSI LGNKLSEIAW HKAGIFKESV 250 260 270 280 290 300 PAFYSPTTTE AEEVLIARAI SKHVPLFQTP PVSAYQFARD ISPGIRGAHQ FSNVSMALQL 310 320 330 340 350 360 VRAWAEKCGF PLPGVPLSTD TSGFNVPLWM CDAIESCRWP GRSQIVSTDR NVTYLLDGAH 370 380 390 400 410 420 TPKSMEACSE WAAEEIVNLK KENVKKILLF QCTADRCPST LIKYLKPLGI SQIVSCPTQL 430 440 450 460 470 480 HSSIDKSADS ANLNASRDEQ AEKANQCVQA WKESLDQPES VTEDQMKVFD CISSAYKFIE 490 500 510 SQAASQEILV LVTGSLHLVG GVLNLAGKGK « Hide
Isoform b [UniParc]. Checksum: CC15C5CF6FCA351D Show »	FASTA	521	57,655
10 20 30 40 50 60 MRIISSIFAP TRGFHSRSTW ILLPTTSSTA CGANQLRMSS EKAVPCYEES VRLLNGLQSN 70 80 90 100 110 120 AATIKKLRVQ RENLQAINLP QCRKYLESLN ISAEDLNALN IIHVSGTKGK GSACAFVESI 130 140 150 160 170 180 LRSQGLRTGF YSSPHLVHVR ERIQVDGQPV SEQMFAEEFF HVYDIIKREH SDNMPAYFKF 190 200 210 220 230 240 LTLLAFRIFV KLNVQVMILE VGIGGEYDCT NVVEKPKVCG VTTLDYDHMS ILGNKLSEIA 250 260 270 280 290 300 WHKAGIFKES VPAFYSPTTT EAEEVLIARA ISKHVPLFQT PPVSAYQFAR DISPGIRGAH 310 320 330 340 350 360 QFSNVSMALQ LVRAWAEKCG FPLPGVPLST DTSGFNVPLW MCDAIESCRW PGRSQIVSTD 370 380 390 400 410 420 RNVTYLLDGA HTPKSMEACS EWAAEEIVNL KKENVKKILL FQCTADRCPS TLIKYLKPLG 430 440 450 460 470 480 ISQIVSCPTQ LHSSIDKSAD SANLNASRDE QAEKANQCVQ AWKESLDQPE SVTEDQMKVF 490 500 510 520 DCISSAYKFI ESQAASQEIL VLVTGSLHLV GGVLNLAGKG K « Hide
Isoform c [UniParc]. Checksum: 661D9BC3CD4895FB Show »	FASTA	484	53,594
10 20 30 40 50 60 MSSEKAVPCY EESVRLLNGL QSNAATIKKL RVQRENLQAI NLPQCRKYLE SLNISAEDLN 70 80 90 100 110 120 ALNIIHVSGT KGKGSACAFV ESILRSQGLR TGFYSSPHLV HVRERIQVDG QPVSEQMFAE 130 140 150 160 170 180 EFFHVYDIIK REHSDNMPAY FKFLTLLAFR IFVKLNVQVM ILEVGIGGEY DCTNVVEKPK 190 200 210 220 230 240 VCGVTTLDYD HMSILGNKLS EIAWHKAGIF KESVPAFYSP TTTEAEEVLI ARAISKHVPL 250 260 270 280 290 300 FQTPPVSAYQ FARDISPGIR GAHQFSNVSM ALQLVRAWAE KCGFPLPGVP LSTDTSGFNV 310 320 330 340 350 360 PLWMCDAIES CRWPGRSQIV STDRNVTYLL DGAHTPKSME ACSEWAAEEI VNLKKENVKK 370 380 390 400 410 420 ILLFQCTADR CPSTLIKYLK PLGISQIVSC PTQLHSSIDK SADSANLNAS RDEQAEKANQ 430 440 450 460 470 480 CVQAWKESLD QPESVTEDQM KVFDCISSAY KFIESQAASQ EILVLVTGSL HLVGGVLNLA GKGK « Hide

References

[1]

"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE INITIATION, ALTERNATIVE SPLICING.
Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081045 Genomic DNA. Translation: CCD68782.1. FO081045 Genomic DNA. Translation: CCD68783.1. FO081045 Genomic DNA. Translation: CCD68784.1.
PIR	T16146.
RefSeq	NP_001022548.1. NM_001027377.1. NP_498291.1. NM_065890.1. NP_498292.1. NM_065891.3.
UniGene	Cel.8718.
3D structure databases
ProteinModelPortal	Q09509.
SMR	Q09509. Positions 32-510.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q09509. 1 interaction.
STRING	Q09509.
Proteomic databases
PRIDE	Q09509.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F25B5.6a; F25B5.6a; F25B5.6.
GeneID	175842.
KEGG	cel:F25B5.6.
UCSC	F25B5.6a. c. elegans.
Organism-specific databases
CTD	175842.
WormBase	F25B5.6a; CE01923; WBGene00017777. F25B5.6b; CE29283; WBGene00017777. F25B5.6c; CE37764; WBGene00017777.
Phylogenomic databases
eggNOG	meNOG04584.
PhylomeDB	Q09509.
Gene expression databases
ArrayExpress	Q09509.
Family and domain databases
InterPro	IPR001645. Folylpolyglutamate_synth. IPR018109. Folylpolyglutamate_synth_CS. IPR023600. Folylpolyglutamate_synth_euk. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K01930.
PANTHER	PTHR11136. Fpolygl_synthtse. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
PIRSF	PIRSF038895. FPGS. 1 hit.
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01499. FolC. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	889900.

Entry information

Entry name

FOLC_CAEEL

Accession

Primary (citable) accession number: Q09509
Secondary accession number(s): Q5DX42, Q95QJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents