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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

sdha-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei256 – 2561FADBy similarity
Binding sitei277 – 2771SubstrateBy similarity
Binding sitei289 – 2891SubstrateBy similarity
Active sitei321 – 3211Proton acceptorBy similarity
Binding sitei388 – 3881SubstrateBy similarity
Binding sitei422 – 4221FADBy similarity
Binding sitei433 – 4331SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 546FADBy similarity
Nucleotide bindingi72 – 8716FADBy similarityAdd
BLAST
Nucleotide bindingi438 – 4392FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. electron transport chain Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_287541. Citric acid cycle (TCA cycle).
REACT_330425. Respiratory electron transport.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
FP
Gene namesi
Name:sdha-1
ORF Names:C03G5.1/D2021.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC03G5.1; CE03917; WBGene00015391; sdha-1.

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrion Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionSequence AnalysisAdd
BLAST
Chaini32 – 646615Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialPRO_0000010339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801Tele-8alpha-FAD histidineBy similarity

Proteomic databases

PaxDbiQ09508.

2D gel databases

World-2DPAGE0020:Q09508.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ09508.
SMRiQ09508. Positions 34-646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1053.
GeneTreeiENSGT00390000010046.
HOGENOMiHOG000160475.
InParanoidiQ09508.
KOiK00234.
OMAiCFHESAR.
OrthoDBiEOG7MH0XJ.
PhylomeDBiQ09508.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRAASNGLR NTVAARSVSL SAANHSDAKR SDIAQYKVVD HAYDAVVVGA
60 70 80 90 100
GGAGLRAAMG LAEGGLKTAV ITKLFPTRSH TVAAQGGINA ALGNMNPDNW
110 120 130 140 150
RWHFYDTVKG SDWLGDQDAI HYMTREAERA VIELENYGMP FSRTTDGKIY
160 170 180 190 200
QRAFGGQSND FGRGGQAHRT CCVADRTGHS LLHTLYGASL QYNCNYFVEY
210 220 230 240 250
FALDLIMENG VCVGVIAMDL EDGTIHRFRS KNTVLATGGY GRAFFSCTSA
260 270 280 290 300
HTCTGDGTAL TARAGINNSD MEFVQFHPTG IYGAGCLITE GSRGEGGYLV
310 320 330 340 350
NSAGERFMER YAPNAKDLAS RDVVSRSMTV EIMEGRGVGP DKDHIYLQLH
360 370 380 390 400
HLPAEQLQQR LPGISETAMI FAGVDVTKEP IPVIPTVHYN MGGVPTNYKG
410 420 430 440 450
QVLNYTPKKG DEVVPGLYAA GECGAHSVHG ANRLGANSLL DLVIFGRACA
460 470 480 490 500
IDILKNTSAG VGVPELPKNA GEASVANIDK LRHNKGDIST AELRLTMQKS
510 520 530 540 550
MQNHAAVFRR GDILKEGVKV LSKLYKDQAH LNVADKGLVW NSDLIETLEL
560 570 580 590 600
QNLLINATQT IVAAENREES RGAHARDDFP DRLDELDYSK PLEGQTKKEL
610 620 630 640
KDHWRKHSII RSNIETGEVS LDYRPVIDTT LDKSETDWVP PKVRSY
Length:646
Mass (Da):70,398
Last modified:August 1, 2002 - v3
Checksum:iCD7CF55A6D26E6D1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641G → E in BAA21637 (PubMed:7739664).Curated
Sequence conflicti267 – 2671N → S in BAA21637 (PubMed:7739664).Curated
Sequence conflicti303 – 3031A → S in BAA21637 (PubMed:7739664).Curated
Sequence conflicti315 – 3151A → S in BAA21637 (PubMed:7739664).Curated
Sequence conflicti463 – 4631V → G in BAA21637 (PubMed:7739664).Curated
Sequence conflicti483 – 4864HNKG → TTRE in BAA21637 (PubMed:7739664).Curated
Sequence conflicti640 – 6401P → A in BAA21637 (PubMed:7739664).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30651 mRNA. Translation: BAA21637.1.
FO080313 Genomic DNA. Translation: CCD62793.1.
PIRiT15398.
T41753.
RefSeqiNP_509446.1. NM_077045.5.
UniGeneiCel.5321.

Genome annotation databases

EnsemblMetazoaiC03G5.1; C03G5.1; WBGene00015391.
GeneIDi181108.
KEGGicel:CELE_C03G5.1.
UCSCiC03G5.1.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30651 mRNA. Translation: BAA21637.1.
FO080313 Genomic DNA. Translation: CCD62793.1.
PIRiT15398.
T41753.
RefSeqiNP_509446.1. NM_077045.5.
UniGeneiCel.5321.

3D structure databases

ProteinModelPortaliQ09508.
SMRiQ09508. Positions 34-646.
ModBaseiSearch...
MobiDBiSearch...

2D gel databases

World-2DPAGE0020:Q09508.

Proteomic databases

PaxDbiQ09508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC03G5.1; C03G5.1; WBGene00015391.
GeneIDi181108.
KEGGicel:CELE_C03G5.1.
UCSCiC03G5.1.2. c. elegans.

Organism-specific databases

CTDi181108.
WormBaseiC03G5.1; CE03917; WBGene00015391; sdha-1.

Phylogenomic databases

eggNOGiCOG1053.
GeneTreeiENSGT00390000010046.
HOGENOMiHOG000160475.
InParanoidiQ09508.
KOiK00234.
OMAiCFHESAR.
OrthoDBiEOG7MH0XJ.
PhylomeDBiQ09508.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
ReactomeiREACT_287541. Citric acid cycle (TCA cycle).
REACT_330425. Respiratory electron transport.

Miscellaneous databases

NextBioi912432.
PROiQ09508.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence comparison between the flavoprotein subunit of the fumarate reductase (complex II) of the anaerobic parasitic nematode, Ascaris suum and the succinate dehydrogenase of the aerobic, free-living nematode, Caenorhabditis elegans."
    Kuramochi T., Hirawake H., Kojima S., Takamiya S., Furushima R., Aoki T., Komuniecki R., Kita K.
    Mol. Biochem. Parasitol. 68:177-187(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiSDHA_CAEEL
AccessioniPrimary (citable) accession number: Q09508
Secondary accession number(s): Q17616, Q34089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: August 1, 2002
Last modified: March 31, 2015
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.