Reviewed, UniProtKB/Swiss-Prot Q09474 (T23O_CAEEL)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

ORF Names:

C28H8.11

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-316265,EBI-316265
gale-1	O62107	1	EBI-316265,EBI-316270
kel-8	O61899	1	EBI-316265,EBI-312469

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Tryptophan 2,3-dioxygenase		PRO_0000072398
Regions
	Region	39 – 43	5	Substrate binding By similarity
	Region	69 – 73	5	Substrate binding By similarity
Sites
	Metal binding	327	1	Iron (heme axial ligand) By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	147	1	Heme By similarity
	Binding site	341	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09474-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8F1E98075CA86A65	FASTA	403	46,716
	10 20 30 40 50 60 MACPYLGSGE LTHRVTFMEG GEECQQGVNK VEMGFGQTYS EYLQLDKILT AQRLKSEADG 70 80 90 100 110 120 QRVDDEHLFI VIHQAHELWF KQIIFDLDNV RKLLNNTIVD ETKTLKIVSG LDRMTKILSL 130 140 150 160 170 180 LTEQITLLDT MSPLDFVDFR KYLTPASGFQ SLQFRVLENK LGVRQERRIK YNAQHYKNVF 190 200 210 220 230 240 NDTDLKTLNV TEEEKSLLTL IESWLERTPG LKSTSEDEGF WIKYEKSVNK YLADLAKQAA 250 260 270 280 290 300 DPSNTEEIAK QLTAEYHKTA DAFQSILDPR QHEQHIRNGN RLLSHDATKG AMMIYFYRDM 310 320 330 340 350 360 PRFSQPYQIL TFLMDIDSLF TKWRYNHVLL VQRMLGAKQG TGGSSGYMYL RSTVSDRYKV 370 380 390 400 FLDLFNLSTW LIPREYIPML SPRMVKTLSE HSNLSHSQSS ESD

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20861 Genomic DNA. Translation: AAA62299.1.
PIR	B88470.
RefSeq	NP_498284.1.
UniGene	Cel.10783
3D structure databases
SMR	Q09474. Positions 37-371.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26438N.
IntAct	Q09474. 4 interactions.
STRING	Q09474.
Proteomic databases
PRIDE	Q09474.
Genome annotation databases
Ensembl	C28H8.11a; C28H8.11a; C28H8.11; Caenorhabditis elegans. [Genome view]
GeneID	175836.
NMPDR	fig|6239.3.peg.9980.
UCSC	C28H8.11c.3. c. elegans.
Organism-specific databases
CTD	175836.
WormBase	WBGene00016201. C28H8.11.
WormPep	C28H8.11. CE01822. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04229.
HOGENOM	HBG647485.
InParanoid	Q09474.
OMA	HYRDNFR.
PhylomeDB	Q09474.
Enzyme and pathway databases
BRENDA	1.13.11.11. 672.
Gene expression databases
ArrayExpress	Q09474.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	889870.

Entry information

Entry name

T23O_CAEEL

Accession

Primary (citable) accession number: Q09474

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project