Q09474 (T23O_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

ORF Names:

C28H8.11

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-316265,EBI-316265
gale-1	O62107	1	EBI-316265,EBI-316270
kel-8	O61899	1	EBI-316265,EBI-312469

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Tryptophan 2,3-dioxygenase		PRO_0000072398
Regions
	Region	39 – 43	5	Substrate binding By similarity
	Region	69 – 73	5	Substrate binding By similarity
Sites
	Metal binding	327	1	Iron (heme axial ligand) By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	147	1	Heme By similarity
	Binding site	341	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09474 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8F1E98075CA86A65	FASTA	403	46,716
	10 20 30 40 50 60 MACPYLGSGE LTHRVTFMEG GEECQQGVNK VEMGFGQTYS EYLQLDKILT AQRLKSEADG 70 80 90 100 110 120 QRVDDEHLFI VIHQAHELWF KQIIFDLDNV RKLLNNTIVD ETKTLKIVSG LDRMTKILSL 130 140 150 160 170 180 LTEQITLLDT MSPLDFVDFR KYLTPASGFQ SLQFRVLENK LGVRQERRIK YNAQHYKNVF 190 200 210 220 230 240 NDTDLKTLNV TEEEKSLLTL IESWLERTPG LKSTSEDEGF WIKYEKSVNK YLADLAKQAA 250 260 270 280 290 300 DPSNTEEIAK QLTAEYHKTA DAFQSILDPR QHEQHIRNGN RLLSHDATKG AMMIYFYRDM 310 320 330 340 350 360 PRFSQPYQIL TFLMDIDSLF TKWRYNHVLL VQRMLGAKQG TGGSSGYMYL RSTVSDRYKV 370 380 390 400 FLDLFNLSTW LIPREYIPML SPRMVKTLSE HSNLSHSQSS ESD

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080703 Genomic DNA. Translation: CCD65972.1.
PIR	B88470.
RefSeq	NP_498284.1. NM_065883.3.
UniGene	Cel.10783.
3D structure databases
ProteinModelPortal	Q09474.
SMR	Q09474. Positions 23-371.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26438N.
IntAct	Q09474. 5 interactions.
MINT	MINT-1048668.
STRING	Q09474.
Proteomic databases
PRIDE	Q09474.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C28H8.11a; C28H8.11a; C28H8.11.
GeneID	175836.
KEGG	cel:C28H8.11.
NMPDR	fig|6239.3.peg.9980.
UCSC	C28H8.11c.3. c. elegans.
Organism-specific databases
CTD	175836.
WormBase	C28H8.11a; CE01822; WBGene00016201.
Phylogenomic databases
eggNOG	meNOG04229.
HOGENOM	HBG647485.
InParanoid	Q09474.
OMA	KLLVDQV.
PhylomeDB	Q09474.
Gene expression databases
ArrayExpress	Q09474.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	889870.

Entry information

Entry name

T23O_CAEEL

Accession

Primary (citable) accession number: Q09474

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project