Q09444 (UBH4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase ubh-4
EC=3.4.19.12

Alternative name(s):
Ubiquitin C-terminal hydrolase family 1 member 4
Ubiquitin thioesterase 4

Gene names

Name:	ubh-4
ORF Names:	C08B11.7

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW omega peptidase activity Inferred from electronic annotation. Source: InterPro ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Probable ubiquitin carboxyl-terminal hydrolase ubh-4		PRO_0000211071
Sites
	Active site	83	1	Nucleophile By similarity
	Active site	158	1	Proton donor By similarity
	Site	173	1	Important for enzyme activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09444 [UniParc]. Last modified December 21, 2004. Version 2. Checksum: F6A658161FC13AD5	FASTA	321	37,120
	10 20 30 40 50 60 MTDAGSWCLI ESDPGVFTEM LRGFGVDGLQ VEELYSLDDD KAMTRPTYGL IFLFKWRQGD 70 80 90 100 110 120 ETTGIPSDKQ NIFFAHQTIQ NACATQALIN LLMNVEDTDV KLGNILNQYK EFAIDLDPNT 130 140 150 160 170 180 RGHCLSNSEE IRTVHNSFSR QTLFELDIKG GESEDNYHFV TYVPIGNKVY ELDGLRELPL 190 200 210 220 230 240 EVAEFQKEQD WIEAIKPVIQ QRMQKYSEGE ITFNLMALVP NRKQKLQEMM ENLIQANENN 250 260 270 280 290 300 ELEEQIADLN KAIADEDYKM EMYRKENNRR RHNYTPFVIE LMKILAKEGK LVGLVDNAYQ 310 320 AAKEKSKLNT DITKLELKRK Q

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46676 Genomic DNA. Translation: CAA86665.2.
PIR	T19070.
RefSeq	NP_495684.2. NM_063283.4.
3D structure databases
ProteinModelPortal	Q09444.
SMR	Q09444. Positions 7-294.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26660N.
IntAct	Q09444. 1 interaction.
MINT	MINT-1058911.
STRING	6239.C08B11.7.
Protein family/group databases
MEROPS	C12.005.
Proteomic databases
PaxDb	Q09444.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C08B11.7; C08B11.7; C08B11.7.
GeneID	174289.
KEGG	cel:CELE_C08B11.7.
UCSC	C08B11.7. c. elegans.
Organism-specific databases
CTD	174289.
WormBase	C08B11.7; CE36913; WBGene00006724; ubh-4.
Phylogenomic databases
eggNOG	NOG321645.
GeneTree	ENSGT00510000046560.
HOGENOM	HOG000203918.
InParanoid	Q09444.
KO	K05610.
OMA	QMFEFDT.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. IPR017390. Ubiquitinyl_hydrolase_UCH37. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PIRSF	PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	883384.

Entry information

Entry name

UBH4_CAEEL

Accession

Primary (citable) accession number: Q09444

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project